Spatially Resolved Immobilization of Peptides by Electrochemical Polymerization after Photolytic Cleavage of a Protecting Group

Abstract

Microstructuring of surfaces: Electrochemical polymerization after removal of a photolabile protecting group (nitrobenzyl group) represents a new method for spatially resolved immobilization of ligands or receptors. Thus, the electropolymerization of 3-hydroxyphenylacetyl peptides such as 1 on electrodes can be controlled by light.

A Biotinylated Conducting Polypyrrole for the Spatially Controlled Construction of an Amperometric Biosensor

“…To preserve their complete biological properties, the immobilization of proteins on electrogenerated polymer films has recently been attempted by the use of an affinity system. − Because of the high affinity of the avidin−biotin interaction (association constant K a = 10 15 M -1 ), this coupling system has been used for protein immobilization on biotinylated polymer films. The attachment of proteins was achieved via the simple formation of an avidin−biotin bridge with biotinylated proteins or avidin-conjugated proteins.…”

Biosensors based on electropolymerized films: new trends

“…This innovative electrochemical method based on avidin-biotin affinity for biomolecule immobilization consisted in the electrogeneration of polymeric films functionalized by biotin moieties that enabled subsequent natural attachment of avidin. The formation of biotinylated surfaces has been attempted by electropolymerization of a biotin derivative functionalized by a phenol group [104,105]. As expected the electropolymerization provided non-conductive films which passivated the electrode surface and prevented further film growth.…”

Fabrication of biosensors by attachment of biological macromolecules to electropolymerized conducting films

“…The efficient coupling of an avidin-conjugated β-galactosidase to the biotinylated film has shown that this kind of polymer films provides biotin sites able to develop specific interactions with avidin. Jung and coworkers have also prepared electropolymerized polyphenol films containing biotin residues [34]. The availability of the polymerized biotin was confirmed with fluorescein-labeled streptavidin.…”