2015
DOI: 10.1007/s10658-015-0600-4
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Spatio-temporal distribution of Grapevine fanleaf virus (GFLV) in grapevine

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Cited by 16 publications
(8 citation statements)
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References 23 publications
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“…In samples Ref9 2/71, LR 5/32, RefDUC 3/8, Ste 4/37 and Zun 5/18, satRNAs could barely be detected at the beginning of the seasons in 2008 and 2011, and could not be detected at all in autumn 2010, even though the same extraction procedure was used for samples collected in spring 2011 and autumn 2010. These results correlate with the seasonal trend of GFLV titres that was reported previously (Jež Krebelj et al ., ). qPCR analyses of phloem samples within the same study also confirmed that reference genes COX and 18S rRNA resulted in constant C q values during the season (data not shown), which means that efficiency of extraction was not dependent on the sampling time period.…”
Section: Discussionmentioning
confidence: 97%
“…In samples Ref9 2/71, LR 5/32, RefDUC 3/8, Ste 4/37 and Zun 5/18, satRNAs could barely be detected at the beginning of the seasons in 2008 and 2011, and could not be detected at all in autumn 2010, even though the same extraction procedure was used for samples collected in spring 2011 and autumn 2010. These results correlate with the seasonal trend of GFLV titres that was reported previously (Jež Krebelj et al ., ). qPCR analyses of phloem samples within the same study also confirmed that reference genes COX and 18S rRNA resulted in constant C q values during the season (data not shown), which means that efficiency of extraction was not dependent on the sampling time period.…”
Section: Discussionmentioning
confidence: 97%
“…S3 ), and at Tf the titre decreased in both cultivars. Krebelj et al 39 reported that in vineyards, the highest GFLV titre was observed at the beginning of the vegetative period in May, and that the virus concentration changed in different grapevine cultivar. Our data agree with their study: the lower GFLV concentration at Tf may be considered a seasonal variation of virus titre, although decrease in viral titre may be caused by PM and DM infection.…”
Section: Discussionmentioning
confidence: 99%
“…This hypothesis was tested by a ‘proof‐of‐concept’ approach using cleft‐grafting, part of the resistance screening, and testing root tissue which mites were unable to reach and feed on. Even though the virus was only present in 50% of the samples tested, this is not unusual as many viruses have uneven distribution in the plant (Harper et al ., ; Krebelj et al ., ; Wells‐Hansen & McManus, ). Pruning of areas with symptoms does not eliminate the virus and should be avoided so as to minimize the persistence of the virus after overwintering in the root system.…”
Section: Discussionmentioning
confidence: 99%