Spatiogenetic characterization of &lt;i&gt;S&lt;/i&gt; receptor kinase (&lt;i&gt;SRK&lt;/i&gt;) alleles in naturalized populations of &lt;i&gt;Raphanus sativus&lt;/i&gt; L. var. &lt;i&gt;raphanistroides&lt;/i&gt; on Yakushima island

Fukushima, Kunio; Kanomata, Toko; Kon, Aoi; Masuko-Suzuki, Hiromi; Ito, Kana; Ogata, Sadayoshi; Takada, Yukyo; Komatsubara, Yukihiro; Nakamura, Tsuyoshi; Watanabe, Toshio; Koizumi, Sanae; Sanuki, Hitoshi; Park, Jong-In; Niikura, Satoshi; Suwabe, Keita; Fujii, Sota; Murase, Kohji; Takayama, Seiji; Suzuki, Go; Watanabe, Masao

doi:10.1266/ggs.20-00066

Cited by 2 publications

(1 citation statement)

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“…Because the SRK and SP11 genes are inherited as a single segregational unit, S alleles are termed S haplotypes (Nasrallah and Nasrallah 1993 ). S -haplotype diversity is determined by sequence polymorphism of SRK and SP11 genes in the S locus and over 100 have been identified ( S 1 , S 2 , S 3 , …, S n ) in the genus Brassica (Nou et al 1993 ; Sakamoto and Nishio 2001 ; Watanabe et al 2000 ; Ockendon 1982 ; Charlesworth et al 2003 ; Paetsch et al 2006 ), and over 30 in Raphanus (Kim and Kim 2019 ; Fukushima et al 2021 ). In Brassica , S haplotypes are classified into two classes (class-I and -II) based on the sequence similarity of the extracellular domain encoded by SRK (and its homologous gene termed SLG ).…”

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confidence: 99%

MLPK function is not required for self-incompatibility in the S29 haplotype of Brassica rapa L.

et al. 2023

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Key message S29 haplotype does not require the MLPK function for self-incompatibility in Brassica rapa. Abstract Self-incompatibility (SI) in Brassicaceae is regulated by the self-recognition mechanism, which is based on the S-haplotype-specific direct interaction of the pollen-derived ligand, SP11/SCR, and the stigma-side receptor, SRK. M locus protein kinase (MLPK) is known to be one of the positive effectors of the SI response. MLPK directly interacts with SRK, and is phosphorylated by SRK in Brassica rapa. In Brassicaceae, MLPK was demonstrated to be essential for SI in B. rapa and Brassica napus, whereas it is not essential for SI in Arabidopsis thaliana (with introduced SRK and SP11/SCR from related SI species). Little is known about what determines the need for MLPK in SI of Brassicaceae. In this study, we investigated the relationship between S-haplotype diversity and MLPK function by analyzing the SI phenotypes of different S haplotypes in a mlpk/mlpk mutant background. The results have clarified that in B. rapa, all the S haplotypes except the S29 we tested need the MLPK function, but the S29 haplotype does not require MLPK for the SI. Comparative analysis of MLPK-dependent and MLPK-independent S haplotype might provide new insight into the evolution of S-haplotype diversity and the molecular mechanism of SI in Brassicaceae.

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Section: Introductionmentioning

confidence: 99%

MLPK function is not required for self-incompatibility in the S29 haplotype of Brassica rapa L.

et al. 2023

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SSR-Sequencing Reveals the Inter- and Intraspecific Genetic Variation and Phylogenetic Relationships among an Extensive Collection of Radish (Raphanus) Germplasm Resources

Wang

Yang

et al. 2021

Biology

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Raphanus has undergone a lengthy evolutionary process and has rich diversity. However, the inter- and intraspecific phylogenetic relationships and genetic diversity of this genus are not well understood. Through SSR-sequencing and multi-analysis of 939 wild, semi-wild and cultivated accessions, we discovered that the European wild radish (EWR) population is separated from cultivated radishes and has a higher genetic diversity. Frequent intraspecific genetic exchanges occurred in the whole cultivated radish (WCR) population; there was considerable genetic differentiation within the European cultivated radish (ECR) population, which could drive radish diversity formation. Among the ECR subpopulations, European primitive cultivated radishes (EPCRs) with higher genetic diversity are most closely related to the EWR population and exhibit a gene flow with rat-tail radishes (RTRs) and black radishes (BRs)/oil radishes (ORs). Among Asian cultivated radishes (ACRs), Chinese big radishes (CBRs) with a relatively high diversity are furthest from the EWR population, and most Japanese/Korean big radishes (JKBRs) are close to CBR accessions, except for a few old Japanese landraces that are closer to the EPCR. The CBR and JKBR accessions are independent of RTR accessions; however, phylogenetic analysis indicates that the RTR is sister to the clade of CBR (including JWR), which suggests that the RTR may share the most recent common ancestry with CBRs and JWRs. In addition, Japanese wild radishes (JWRs), (namely, R. sativus forma raphanistroides) are mainly scattered between CBRs and EPCRs in PCoA analysis. Moreover, JWRs have a strong gene exchange with the JKBR, OR and RTR subpopulations. American wild radishes (AWRs) are closely related to European wild and cultivated radishes, and have a gene flow with European small radishes (ESRs), suggesting that the AWR developed from natural hybridization between the EWR and the ESR. Overall, this demonstrates that Europe was the origin center of the radish, and that Europe, South Asia and East Asia appear to have been three independent domestication centers. The EPCR, AWR and JWR, as semi-wild populations, might have played indispensable transitional roles in radish evolution. Our study provides new perspectives into the origin, evolution and genetic diversity of Raphanus and facilitates the conservation and exploitation of radish germplasm resources.

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Spatiogenetic characterization of <i>S</i> receptor kinase (<i>SRK</i>) alleles in naturalized populations of <i>Raphanus sativus</i> L. var. <i>raphanistroides</i> on Yakushima island

Cited by 2 publications

References 65 publications

MLPK function is not required for self-incompatibility in the S29 haplotype of Brassica rapa L.

MLPK function is not required for self-incompatibility in the S29 haplotype of Brassica rapa L.

SSR-Sequencing Reveals the Inter- and Intraspecific Genetic Variation and Phylogenetic Relationships among an Extensive Collection of Radish (Raphanus) Germplasm Resources

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