Spatiotemporal distributions and environmental drivers of diversity and community structure of nosZ-type denitrifiers and anammox bacteria in sediments of the Bohai Sea and North Yellow Sea, China

Abstract

Denitrifi cation and anammox processes are major nitrogen removal processes in coastal ecosystems. However, the spatiotemporal dynamics and driving factors of the diversity and community structure of involved functional bacteria have not been well illustrated in coastal environments, especially in human-dominated ecosystems. In this study, we investigated the distributions of denitrifi ers and anammox bacteria in the eutrophic Bohai Sea and the northern Yellow Sea of China in May and November of 2012 by constructing clone libraries employing nosZ and 16S rRNA gene biomarkers. The diversity of nosZ-denitrifi er was much higher at the coastal sites compared with the central sites, but not signifi cant among basins or seasons. Alphaproteobacteria were predominant and prevalent in the sediments, whereas Betaproteobacteria primarily occurred at the site near the Huanghe (Yellow) River estuary. Anammox bacteria Candidatus Scalindua was predominant in the sediments, and besides, Candidatus Brocadia and Candidatus Kuenenia were also detected at the site near the Huanghe River estuary that received strong riverine and anthropogenic impacts. Salinity was the most important in structuring communities of nosZdenitrifi er and anammox bacteria. Additionally, anthropogenic perturbations (e.g. nitrogen overloading and consequent high primary productivity, and heavy metal discharges) contributed signifi cantly to shaping community structures of denitrifi er and anammox bacteria, suggesting that anthropogenic activities would infl uence and even change the ecological function of coastal ecosystems.

Salinity-Linked Denitrification Potential in Endorheic Lake Bosten (China) and Its Sensitivity to Climate Change

“…Furthermore, the influence of salinity on denitrification depends on the long-term salinity adaptation of denitrifying microorganisms (Franklin et al, 2017 ; Wang et al, 2018a ; Murphy et al, 2020 ). Many studies have shown that salinity is the most important factor in structuring communities of denitrifying microorganisms, such as nosZ -denitrifiers (Cai et al, 2019 ; Wang et al, 2019 ; Han et al, 2021 ), and that salinity can affect denitrification rates by altering the abundance and diversity of denitrifying microorganism. In Lake Bosten, there was a significant correlation between the abundance of the nirS and nosZI genes ( r = 0.79, p < 0.01).…”

On anammox activity at low temperature: Effect of ladderane composition and process conditions

“…In the literature, "Ca. Scalindua" is an organism that has mainly been recovered from marine environments [51,52]. We detected "Ca.…”