2015
DOI: 10.1016/j.biomaterials.2014.10.022
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Spatiotemporal dynamics of microRNA during epithelial collective cell migration

Abstract: MicroRNAs (miRNAs) are small, noncoding RNAs variably involved in a wide variety of developmental and regenerative programs. Techniques for monitoring the spatiotemporal expression of miRNA in living cells are essential to elucidate the roles of miRNA during these complex regulatory processes. The small size, low abundance, sequence similarity, and degradation susceptibility of miRNAs, however, make their detection challenging. In this study, we detail a double-stranded locked nucleic acid (dsLNA) probe for de… Show more

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Cited by 25 publications
(29 citation statements)
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“…To investigate leader cells, we applied a double-stranded locked nucleic acid (dsLNA) assay for detecting gene expression at the single cell level 17 , 27 , 28 and compared the acquired gene expression with other leader cell characteristics. Leader cells are typically characterized by their phenotypes, including enlarged morphology and active lamellipodium on the migration tip.…”
Section: Resultsmentioning
confidence: 99%
“…To investigate leader cells, we applied a double-stranded locked nucleic acid (dsLNA) assay for detecting gene expression at the single cell level 17 , 27 , 28 and compared the acquired gene expression with other leader cell characteristics. Leader cells are typically characterized by their phenotypes, including enlarged morphology and active lamellipodium on the migration tip.…”
Section: Resultsmentioning
confidence: 99%
“…The LNA probe was labeled with a fluorophore (6-FAM) at the 5’ end for fluorescence detection. The design process of LNA probes was reported previously 25, 26 . Briefly, the LNA probe was designed to be complementary to the loop region of the target mRNA structure.…”
Section: Methodsmentioning
confidence: 99%
“…A probe-modified technique can improve the hybridization efficiency and detection sensitivity. Kin Wong et al [42] have reported an intracellular miRNAs detection strategy using a doublestranded locked nucleic acid (dsLNA) probe, which showed high performance in detecting the dynamic regulation and dose-dependent modulation of miRNAs.…”
Section: Mirna Imaging Analysismentioning
confidence: 99%