Spatiotemporal regulation of fibroblast growth factor signal blocking for endoderm formation in Xenopus laevis

Cha, Sang‐Wook; Lee, Jongwoo; Hwang, Yoo-Seok; Chae, Jeong-Pil; Park, Kwon Moo; Cho, Hee Jung; Kim, Dong Sun; Bae, Yong Chul; Park, Mae Ja

doi:10.3858/emm.2008.40.5.550

Cited by 3 publications

(2 citation statements)

References 24 publications

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“…Otx1 collaborates with Vegt to activate endodermal gene expression and to repress mesoderm formation. Repression of mesodermal gene expression appears to occur at least in part through Otx1 downregulation of mesodermal Fgf signaling, which inhibits endodermal formation in both Xenopus and zebrafish (Cha et al, 2004, 2008; Poulain et al, 2006). The mechanism for toggling the dual functions of Otx1 on different genes remains unclear, but 2 mechanisms have been proposed.…”

Section: Discussionmentioning

confidence: 99%

Endodermal Maternal Transcription Factors Establish Super-Enhancers during Zygotic Genome Activation

et al. 2019

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SUMMARY Elucidation of the sequence of events underlying the dynamic interaction between transcription factors and chromatin states is essential. Maternal transcription factors function at the top of the regulatory hierarchy to specify the primary germ layers at the onset of zygotic genome activation (ZGA). We focus on the formation of endoderm progenitor cells and examine the interactions between maternal transcription factors and chromatin state changes underlying the cell specification process. Endoderm-specific factors Otx1 and Vegt together with Foxh1 orchestrate endoderm formation by coordinated binding to select regulatory regions. These interactions occur before the deposition of enhancer histone marks around the regulatory regions, and these TFs recruit RNA polymerase II, regulate enhancer activity, and establish super-enhancers associated with important endodermal genes. Therefore, maternal transcription factors Otx1, Vegt, and Foxh1 combinatorially regulate the activity of super-enhancers, which in turn activate key lineage-specifying genes during ZGA.

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Section: Discussionmentioning

confidence: 99%

Endodermal Maternal Transcription Factors Establish Super-Enhancers during Zygotic Genome Activation

et al. 2019

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“…In fact, FGF renders the endoderm with the competence to take a liver fate by activating MAPK [27]. However, it is found that, in Xenopus embryos, blocking of FGF signaling by injecting DNFR before stage 10.5 expands the endoderm areas and results in vegetal cells fated into ectoderm to adopt an endoderm fate [28]. Consistent with that observed in Xenopus laevis, the Fgf signaling has a repressive effect on endoderm formation in early gastrulation stage of zebrafish, and blocking or activation of Fgf signaling can lead to an increase of the number of sox17-expressing cells or interference of endoderm formation [29].…”

Section: Fgf Signaling and Liver Initiationmentioning

confidence: 94%

The role of mesodermal signals during liver organogenesis in zebrafish

Niu

Shi

Peng

2010

Sci. China Life Sci.

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Three germ cell layers, the ectoderm, mesoderm and endoderm, are established during the gastrulation stage. All cell types in different organs and tissues are derived from these 3 germ cell layers at later stages. For example, skin epithelial cells and neuronal cells are derived from the ectoderm, while endothelial cells and muscle cells from the mesoderm and lung, and intestine epithelial cells from the endoderm. While in a normal situation different germ cells are destined to specific cell fates in different organs and tissues, each type of germ cells or its derivatives also produce extracellular signaling molecules to direct and facilitate the specification and differentiation of other germ cells during organogenesis. Liver is derived from the endoderm, but completion of liver organogenesis is regulated at different levels. While the pan-endoderm factors (e.g. FoxA and Gata families) and liver specific factors (e.g. Prox1 and Hhex) are essential intrinsic factors for endoderm cells to be differentiated into hepatoblasts, the role of signals produced by neighboring mesoderm cells for liver organogenesis is equally important. This review summarizes recent progress in studying the role of Bone morphogenetic proteins (Bmp), Fibroblast growth factors (Fgf), retinoic acid (RA) and Wingless and Int (Wnt), the 4 types of signaling molecules produced by the mesoderm cells, in liver organogenesis in zebrafish.

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Endoderm Formation in Vertebrates

Wardle¹

2020

Encyclopedia of Life Sciences

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During vertebrate embryogenesis, endoderm, the inner‐most germ layer, will form the gut and associated organs, including liver and pancreas. Studies in the zebrafish, Xenopus and mouse have shown that the signalling pathways and transcription factors that regulate endodermal cell fate in the early embryo are largely conserved in vertebrates. Evidence from these model systems indicates Nodal signalling induces endoderm, in combination with the activity of transcription factors from the Mix, Gata, Sox and T‐box families. Other signalling pathways, such as Fgf, Bmp and Wnt, ensure the correct balance of mesoderm and endoderm is formed during gastrulation. Key Concepts Endoderm formation is initiated during mid‐blastula stages in zebrafish and Xenopus . High levels of Nodal signalling induce endoderm formation. A conserved set of transcription factors, of the Sox, Mix, Gata and T‐box families, act in a network with Nodal to establish endodermal fate during gastrulation. The role of these factors and pathways downstream of Nodal signalling in early endoderm formation is mostly conserved; however, there are some species‐specific differences. Other signalling pathways, notably the Fgf, Bmp and Wnt pathways, regulate the amount of endoderm that forms versus other germ layers.

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Spatiotemporal regulation of fibroblast growth factor signal blocking for endoderm formation in Xenopus laevis

Cited by 3 publications

References 24 publications

Endodermal Maternal Transcription Factors Establish Super-Enhancers during Zygotic Genome Activation

Endodermal Maternal Transcription Factors Establish Super-Enhancers during Zygotic Genome Activation

The role of mesodermal signals during liver organogenesis in zebrafish

Endoderm Formation in Vertebrates

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