Spatiotemporal regulation of keratin 5 and 17 in the axolotl limb

Moriyasu, Miyuki; Makanae, Aki; Satoh, Akira

doi:10.1002/dvdy.23839

Cited by 22 publications

(16 citation statements)

References 57 publications

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“…In addition to a distinct morphology and paucity of proliferating cells, several genetic markers of the wound epidermis have been identified in salamanders and newts 21 22 23 . The best characterized of these markers are keratins 5 and 17 whose expression is upregulated in the wound epidermis following amputation and persists through re-differentiation 21 . In response to injury, we found presence of keratin 17 throughout the neo-epidermis of A. cahirinus and M. musculus ( Fig.…”

Section: Resultsmentioning

confidence: 99%

Comparative analysis of ear-hole closure identifies epimorphic regeneration as a discrete trait in mammals

et al. 2016

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Why mammals have poor regenerative ability has remained a long-standing question in biology. In regenerating vertebrates, injury can induce a process known as epimorphic regeneration to replace damaged structures. Using a 4-mm ear punch assay across multiple mammalian species, here we show that several Acomys spp. (spiny mice) and Oryctolagus cuniculus completely regenerate tissue, whereas other rodents including MRL/MpJ ‘healer' mice heal similar injuries by scarring. We demonstrate ear-hole closure is independent of ear size, and closure rate can be modelled with a cubic function. Cellular and genetic analyses reveal that injury induces blastema formation in Acomys cahirinus. Despite cell cycle re-entry in Mus musculus and A. cahirinus, efficient cell cycle progression and proliferation only occurs in spiny mice. Together, our data unite blastema-mediated regeneration in spiny mice with regeneration in other vertebrates such as salamanders, newts and zebrafish, where all healthy adults regenerate in response to injury.

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Section: Resultsmentioning

confidence: 99%

Comparative analysis of ear-hole closure identifies epimorphic regeneration as a discrete trait in mammals

et al. 2016

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“…Two groups of genes were selected. The first one consists of 5 randomly-selected genes previously well known as DE in axolotl limb regeneration ( MAPK6, Keratin17, TGFB1, MMP13 ), and Coagulation factorXIII which is more evidently expressed in Cynops orientalis limb and Pleurodelinae (newt) lens regeneration 57,82–86 . The second group consists of four randomly-selected genes from the ones uniquely found as DE by our methodology ( CTPS , IFNAR1 , PCDHGC3 , METTL2A ).…”

Section: Resultsmentioning

confidence: 99%

Integrative Analysis of Axolotl Gene Expression Data from Regenerative and Wound Healing Limb Tissues

Sibai

Parlayan

Tuğlu

et al. 2019

Sci Rep

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Axolotl (Ambystoma mexicanum) is a urodele amphibian endowed with remarkable regenerative capacities manifested in scarless wound healing and restoration of amputated limbs, which makes it a powerful experimental model for regenerative biology and medicine. Previous studies have utilized microarrays and RNA-Seq technologies for detecting differentially expressed (DE) genes in different phases of the axolotl limb regeneration. However, sufficient consistency may be lacking due to statistical limitations arising from intra-laboratory analyses. This study aims to bridge such gaps by performing an integrative analysis of publicly available microarray and RNA-Seq data from axolotl limb samples having comparable study designs using the “merging” method. A total of 351 genes were found DE in regenerative samples compared to the control in data of both technologies, showing an adjusted p-value < 0.01 and log fold change magnitudes >1. Downstream analyses illustrated consistent correlations of the directionality of DE genes within and between data of both technologies, as well as concordance with the literature on regeneration related biological processes. qRT-PCR analysis validated the observed expression level differences of five of the top DE genes. Future studies may benefit from the utilized concept and approach for enhanced statistical power and robust discovery of biomarkers of regeneration.

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“…When comparing metamorphic to neotenic samples at 7 dpa, Keratin 5 (Krt5) was found relatively up-regulated in neotenic samples. Up-regulation of Krt5 was shown to be an important regulator in regenerating blastema (Moriyasu et al 2012) , while its downregulation is observed in differentiating limbs. Expression levels of aggrecan (Acan) is also increased during cell differentiation (Takagi et al 2008) , which was found down-regulated in neotenic at 7 dpa compared to metamorphic samples.…”

Section: Discussionmentioning

confidence: 99%

Comparison of Gene Expression During Early Phases of Limb Regeneration Between Regeneration-permissive Neotenic and Regeneration-deficient Metamorphic Axolotl

Sibai

Altuntaş

et al. 2019

Preprint

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The axolotl (Ambystoma Mexicanum) salamander, an urodele amphibian, has an exceptional regenerative capacity to fully restore an amputated limb throughout the life-long lasting neoteny. By contrast, when axolotls are experimentally induced to metamorphosis, attenuation of the limb's regenerative competence is noticeable. Here, we sought to discern the proteomic profiles of the early stages of blastema formation of neotenic and metamorphic axolotls after limb amputation by means of LC-MS/MS technology. We quantified a total of 714 proteins having an adjusted p < 0.01 with FC greater or equal to 2 between two conditions. Principal component analysis revealed a conspicuous clustering between neotenic and metamorphic samples at 7 days post-amputation. Different set of proteins was identified as differentially expressed at all of the time points (1, 4, and 7 days post-amputations against day0) for neotenic and metamorphic stages. Although functional enrichment analyses underline the presence of common pathways between regenerative and nonregenerative stages, cell proliferation and its regulation associated pathways, immune system related pathways and muscle tissue and ECM remodeling and degradation pathways were represented at different rate between both stages. To validate the proteomics results and provide evidence for the putative link between immune system activity and regenerative potential, qRT-PCR for selected genes was performed.

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Spatiotemporal regulation of keratin 5 and 17 in the axolotl limb

Cited by 22 publications

References 57 publications

Comparative analysis of ear-hole closure identifies epimorphic regeneration as a discrete trait in mammals

Comparative analysis of ear-hole closure identifies epimorphic regeneration as a discrete trait in mammals

Integrative Analysis of Axolotl Gene Expression Data from Regenerative and Wound Healing Limb Tissues

Comparison of Gene Expression During Early Phases of Limb Regeneration Between Regeneration-permissive Neotenic and Regeneration-deficient Metamorphic Axolotl

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