Speciation of selenium in cells by HPLC-ICP-MS after (on-chip) magnetic solid phase extraction

Chip-based array magnetic solid phase microextraction on-line coupled with inductively coupled plasma mass spectrometry for the determination of trace heavy metals in cells

“…Metals and metal-containing compounds interfere with functions of the central nervous system (CNS), the 25 haematopoietic system, liver and kidneys 1 .They can also influence cell signal components such as growth factor receptors, G-proteins, mitogen activated protein (MAP) kinases and nuclear transcription [2][3][4][5][6] . Metals and metal-containing compounds interfere with functions of the central nervous system (CNS), the 25 haematopoietic system, liver and kidneys 1 .They can also influence cell signal components such as growth factor receptors, G-proteins, mitogen activated protein (MAP) kinases and nuclear transcription [2][3][4][5][6] .…”

“…In our previous works, chip-based magnetic solid phase microextraction (chip-based MSPME) systems were fabricated and combined with electrothermal vaporization (ETV)-ICP-MS and high performance liquid chromatography (HPLC)-ICP-MS for trace 5 elements and selenium species analysis in cells 24,25 . These chip based systems provides low limits of detection (LODs) and high enrichment factors, and is able to detect endogenous levels of Cd, Hg and Pb in ca 5000 HepG2 cells 24 or selenium species in ca 800 10 selenium-enriched yeast cells 25 .…”

“…However, the ETV sample introduction system is not available for each lab and the drawbacks associated with these off-line systems, such as elaborate manual operations, possible analyte loss and contamination from 15 exogenous reagents or employed vials, still harassed us. Yin et al 26,27 proposed a micro-fluidic sub-microliter/nanoliter sample introduction system for ICP-MS determination of Cd and Pb in Chinese rice wine 26 25 and Pt in human plasma 27 , but no sample pretreatment was involved in this combination system. In recent years, 20 great efforts have been made in the design and fabrication of on-line chip-ICP-MS system.…”

Magnetic solid-phase extraction using carbon nanotubes as sorbents: A review

“…strategy is the on-chip M-SPE method [69][70][71]. In these cases, integrated microfluidic chips, which consist of reaction, mixing and extraction units along with two magnets, were designed and fabricated for M-SPE.…”

Recent trends in nanomaterial-based microanalytical systems for the speciation of trace elements: A critical review

“…When using a highly sensitive detector, high EFs and PFs are relatively unimportant. Analytical instruments that have been used to determine trace elements include inductively coupled plasma atomic emission spectrometry (ICP-AES) and inductively coupled plasma optical emission spectrometry (ICP-OES) [45,67,68]; inductively coupled plasma mass spectrometry (ICP-MS) [3,28,29,31,[69][70][71][72][73][74][75][76][77][78][79]; electrothermal atomic absorption spectrometry (ET-AAS) and graphite furnace atomic absorption spectrometry (GF-AAS) [4,12,33,36,38,48,[80][81][82][83][84][85][86][87]; hydride-generation atomic absorption spectrometry (HG-AAS) [88]; flame atomic absorption spectrometry (FAAS) [35, 89,90]; and atomic fluorescence spectrometry (AFS) [27,74,[91][92][93][94][95].…”