Species and lineage identification for yellowfin Thunnus albacares and bigeye T. obesus tunas using two independent multiplex PCR assays

Higashi, Ryouichi; Sakuma, Kay; Chiba, Satoru; Suzuki, Nobuaki; Chow, Seinen; Semba, Yasuko; Okamoto, Hiroaki; Nohara, Kenji

doi:10.1007/s12562-016-1027-3

Cited by 7 publications

(5 citation statements)

References 27 publications

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“…Conversely, in the canned BET, even if NS occurred, only one sample was misidentified as SKJ. For BET samples, misidentification was rarer than in other species because NS did not occur in diagnostic positions [ 44 ]. This contrasts with LOT samples, in which a large number of NS at most of the diagnostic positions impacted species identification of defrosted and cooked individuals.…”

Section: Discussionmentioning

confidence: 99%

Canning Processes Reduce the DNA-Based Traceability of Commercial Tropical Tunas

Pecoraro

Crobe

Ferrari

et al. 2020

Foods

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Canned tuna is one of the most widely traded seafood products internationally and is of growing demand. There is an increasing concern over the vulnerability of canned tuna supply chains to species mislabelling and fraud. Extensive processing conditions in canning operations can lead to the degradation and fragmentation of DNA, complicating product traceability. We here employed a forensically validated DNA barcoding tool (cytochrome b partial sequences) to assess the effects of canning processes on DNA degradation and the identification of four tropical tuna species (yellowfin, bigeye, skipjack and longtail tuna) collected on a global scale, along their commercial chains. Each species was studied under five different canning processes i.e., freezing, defrosting, cooking, and canning in oil and brine, in order to investigate how these affect DNA-based species identification and traceability. The highest percentage of nucleotide substitutions were observed after brine-canning operations and were greatest for yellowfin and skipjack tuna. Overall, we found that DNA degradation significantly increased along the tuna canning process for most specimens. Consequently, most of the specimens canned in oil or brine were misidentified due to the high rate of nucleotide substitution in diagnostic sequences.

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Section: Discussionmentioning

confidence: 99%

Canning Processes Reduce the DNA-Based Traceability of Commercial Tropical Tunas

Pecoraro

Crobe

Ferrari

et al. 2020

Foods

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“…() yielded low COI genetic distance among three Thunnus species, T. albacares , T. atlanticus and T. tonggol and argued that COI was not useful in delimiting these closely related species. However, genetic markers had been applied to discriminate Thunnus species in several studies (Higashi et al., ; Kumar, Kocour, & Kunal, ; Pedrosa‐Gerasmio, Babaran, & Santos, ), and three pairs of species ( T. thynnus and T. alalunga , T. thynnus and T. orientalis , T. alalunga and T. albacares ) by D‐loop region and the first internal transcribed spacer region (Mitchell & Hellberg, ). In the present study, although low interspecific divergence was found among the three species, network analyses showed that T. obesus (eight sequences) shared a haplotype with T. albacares , while T. tonggol did not share haplotypes with the other two species.…”

Section: Discussionmentioning

confidence: 99%

Developing aDNAbarcode library for perciform fishes in the South China Sea: Species identification, accuracy and cryptic diversity

Hou

Chen

et al. 2017

Molecular Ecology Resources

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“…Fish length for both size categories was measured to the nearest 0.1 cm using a caliper. Species identification was performed using a molecular biological technique, namely, multiplex PCR (Higashi et al 2016), using small portions of each specimen. In previous studies, the length of large juveniles is usually expressed as FL, whereas SL is usually used for small juveniles.…”

Section: Field Sampling and Laboratory Treatmentmentioning

confidence: 99%

Connectivity in juvenile yellowfin tuna Thunnus albacares between temperate and tropical regions of the western Pacific Ocean

Satoh¹,

Katayama²,

Tanabe³

et al. 2023

Mar. Ecol. Prog. Ser.

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Yellowfin tuna Thunnus albacares is a commercially important fish species widely distributed between 40° S and 40° N. Mixing rates between tropical and temperate areas of the western Pacific Ocean were investigated using stable carbon (δ13Cotolith) and oxygen isotopes (δ18Ootolith) in whole otoliths of small juveniles (mean: 5.8 cm standard length, SL) and in otolith cores of large juveniles (32.4 cm SL). The density distribution of δ18Ootolith values for small juveniles showed a significant difference between the areas, whereas patterns for large juveniles were almost identical between areas. In addition, modal values for large juveniles spanned several small juvenile modes. The density distribution of δ13Cotolith values for small juveniles had a single significantly different mode for each area, whereas the distribution of δ13Cotolith values for large juveniles in these areas was bimodal, particularly in the temperate area; these modes corresponded to those for small juveniles in each area. These modal patterns indicate that the majority of large juveniles captured in the temperate area have tropical origins, and mixing from tropical to temperate areas occurs between the areas before recruitment to fisheries in each area. These connectivity patterns during early life history can contribute to refinement of stock assessments of this commercially valuable species.

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Species and lineage identification for yellowfin Thunnus albacares and bigeye T. obesus tunas using two independent multiplex PCR assays

Cited by 7 publications

References 27 publications

Canning Processes Reduce the DNA-Based Traceability of Commercial Tropical Tunas

Canning Processes Reduce the DNA-Based Traceability of Commercial Tropical Tunas

Developing aDNAbarcode library for perciform fishes in the South China Sea: Species identification, accuracy and cryptic diversity

Connectivity in juvenile yellowfin tuna Thunnus albacares between temperate and tropical regions of the western Pacific Ocean

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