Species C is Predominant in Chinese Children with Acute Respiratory Adenovirus Infection

Abstract

72 hours after the onset of symptoms, and then transported to the Network Laboratory of Chinese National Influenza Surveillance in Wuxi CDC. Seven viruses were detected with real time PCR methods. 4 All Hadvpositive nasopharyngeal aspirate specimens were inoculated into Hep-2 cells and were observed daily until an evident cytopathic effect was observed. Partial hexon genes were amplified and sequenced. 4 Phylogenetic trees were inferred by the neighborjoining methods with bootstrap test of 1000 replicates using software MEGA 6.0.Among 1035 specimens, 343 were positive for at least 1 of the 7 common respiratory viruses. Influenza virus predominated, accounting for 36.7% (126/343), followed by parainfluenza virus (23.6%, 81/343), respiratory syncytial viruses (14.9%, 51/343), Hadv (10.8%, 37/343), human metapneumovirus (9.6%, 33/343) and human bocavirus (2.0%, 7/343). One subject was co-infected with Hadv and H.The prevalence of viral pathogenic ARTI caused by Hadv in Wuxi City is higher than in other regions of China. 2,3,5 More children were involved in this study than in other studies.Of 37 Hadv specimens, 30 sequences were submitted into GenBank with accession number KF954030-KF954059. Phylogenetic analysis showed that 20 (67%) belonged to Species Hsdv-C. Ten sequences were Species Hsdv-B. Previous studies showed that Hadv-B was predominant, accounting for 77.9% of cases. 5 In this study, Hsdv-C was predominant. Moreover, as shown in Figure, Supplemental Digital Content 1, http://links.lww.com/INF/C181, the sequences isolated from the same year did not cluster together, implying that these cases were sporadic.In conclusion, our study highlight that the species of Hadv circulating in China differ among various regions.

Development and Application of a Fast Method to Acquire the Accurate Whole-Genome Sequences of Human Adenoviruses

“…However, it could lead to a severe outcome in immunocompromised hosts (Lion, 2014). Recently, more and more type 2 adenoviruses were identified in pediatric infectious disease (Ma et al, 2015;Dhingra et al, 2019), some of which were recombinants with other members in HAdV-C (Wang et al, 2016;Dhingra et al, 2019;Zhang and Huang, 2019;Zhao et al, 2019). In this study, the complete genomic sequence of HAdV-C2 strain HK91 was obtained by our method.…”

Human adenovirus species C recombinant virus continuously circulated in China

“…HAdV is responsible for about 5% to 10% of acute respiratory infections in pediatric patients less than five years old worldwide 23 . In particular, HAdV-C viruses are the most prevalent 24,25 . Homologous recombination plays an important role in the molecular evolution of HAdVs and has been previously confirmed for HAdV-A, HAdV-B, and HAdV-D 26 .…”

Genome Analysis of A Novel Recombinant Human Adenovirus Type 1 in China

“…In conclusion, we propose that the SH2016 strain is a novel intratypic HAdV-C strain and may be an etiological agent of SARI. On the basis of their complete genome sequences, it arose through the recombination of two HAdV genotypes, HAdV-1 and HAdV-2, which frequently cause respiratory infection 9 , 21 – 23 . Whether the emergence of recombination strain might increase virulence, thereby posing a new global challenge with regard to acute respiratory diseases in the near future, warrants further investigation.…”

“…Among of these, members of the HAdV-B group (types 3, 7, 11, 14, 16, 21, 34, 35, 50 and 55) and HAdV-C group (types 1, 2, 5, 6 and 57) cause a variety of typically acute respiratory diseases. Especially, HAdV-C group could cause severe bronchiolitis or pneumonia in the early childhood 8 , 9 .…”