Species Distribution and Antifungal Susceptibility Pattern of Candida Recovered from Intensive Care Unit Patients, Vietnam National Hospital of Burn (2017–2019)

Sinh, Cao Truong; Loi, Cao Ba; Minh, Nguyen Thai; Lam, N N; Quang, Dinh Xuan; Đỗ, Quyết; Anh, Do Ngoc; Hien, Truong Thi Thu; Su, Hoang Xuan; Le, Tran-Anh

doi:10.1007/s11046-021-00569-7

Cited by 5 publications

(4 citation statements)

References 38 publications

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“…C. albicans remains the most common Candida species reported worldwide [6,7,16,17,30,34], while the distribution relative to other species varies in different geographical areas with C. glabrata the most frequent species in Northern Europe and the USA [6], C. parapsilosis in Italy, Spain and Brazil [6,29], and C. tropicalis in Asian countries [17,30,35], especially in tropical southeast Asian countries [13,[36][37][38]. In this study, C. albicans was dominant in mucosal diseases and it was the most isolated species in invasive candidiasis, even if no a statistically significant difference was observed compared to NAC.…”

Section: Discussionmentioning

confidence: 99%

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Azole non-susceptible C. tropicalis and polyclonal spread of C. albicans in Central Vietnam hospitals

Ngo

Santona

Fiamma

et al. 2023

J Infect Dev Ctries

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Introduction: Candida spp. are responsible for infections ranging from local to systemic, and resistance to antifungal first-line therapy is increasing in non-albicans Candida species. We aimed to determine the etiology of candidiasis and the antifungal resistance of Candida spp. isolated in Hue hospitals, Central-Vietnam. Methods: Species identification was performed by matrix-assisted laser desorption/ionization-time of flight mass spectrometry supported by fungal internal-transcribed-spacer amplification and sequencing. Antifungal susceptibility testing was performed by disk diffusion method and minimum inhibitory concentrations of azoles, caspofungin, and amphotericin B against C. tropicalis were determined by broth microdilution. Polymorphism of erg11 gene associated with fluconazole resistance was carried out by polymerase chain reaction and sequencing. Multilocus sequence typing (MLST) was used for typing selected C. albicans isolates. Results: Overall, 196 Candida isolates were detected, mostly C. albicans (48%), followed by C. tropicalis (16%), C. parapsilosis (11%), C. glabrata (9%), C. orthopsilosis (6%) and to a lesser extent another eight species. High rates of resistance to fluconazole and voriconazole (18.8%) were observed in C. tropicalis with five isolates co-resistant to both agents. Y132F and S154F missense mutations in the ERG11 protein were associated with fluconazole-resistance in C. tropicalis (67.7%). Resistance to caspofungin was found in one isolate of C. albicans. MLST identified a polyclonal population of C. albicans with multiple diploid sequence types, and with few lineages showing potential nosocomial spread. Conclusions: Resistance to triazole agents should be considered in C. tropicalis infections in the studied hospitals, and surveillance measures taken to avoid Candida diffusion.

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Section: Discussionmentioning

confidence: 99%

“…In Vietnam, fungal opportunistic diseases are growing due to the increased number of immunocompromised patients [9,10]. Although physicians have dealt with Candida infections more frequently, there are limited systematic epidemiological data on Candida in the country [11][12][13], even less from Central Vietnam.…”

Section: Introductionmentioning

confidence: 99%

Azole non-susceptible C. tropicalis and polyclonal spread of C. albicans in Central Vietnam hospitals

Ngo

Santona

Fiamma

et al. 2023

J Infect Dev Ctries

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“…Specifically C. tropicalis and C. albicans were very often targeted by our yeast isolates. As those yeasts are the most frequently isolated Candida species in candidiasis/candidemia patients in the Asia-Pacific-region, [122][123][124] further research on novel antifungals with certain level of specificity towards these pathogenic yeasts could be promising for the development of fungal vaccines, drugs and other biopharmaceuticals.…”

Section: Medical Applicationsmentioning

confidence: 99%

The antifungal capacity of a 681-membered collection of environmental yeast isolates

Maciá Valero,

Tabatabaeifar,

Billerbeck

2024

Preprint

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Fungal pathogens threaten human health and food security, with resistance reported across limited antifungal classes. Novel strategies to control these pathogens and food spoilers are urgently needed.Environmental yeasts provide a functionally diverse, yet underexploited potential for fungal control based on their natural competition via the secretion of iron siderophores, killer toxins (proteins) or other small molecules like volatile organic compounds or biosurfactants. However, there is a lack of standardized workflows to systematically access application- relevant yeast-based compounds and understand their molecular functioning.Towards this goal, we developed a workflow to identify and characterize yeast isolates that are active against relevant human and plant pathogens and spoilage yeasts, herein focusing on discovering yeasts that produce potential killer toxins. The workflow includes the classification of the secreted molecules and cross-comparison of their antifungal capacity using an independent calibrant.Our workflow delivered a collection of 681 yeasts of which 212 isolates (31%) displayed antagonism against at least one of our target strains. While 50% of the active yeasts showed iron-depended antagonism, likely due to siderophore production, more than 25% are potentially secreting a toxic protein. Those killer yeast candidates clustered within ten species, showed target profiles from narrow- to broad spectrum, and several showed a broad pH and temperature activity profile.Given the tools for yeast biotechnology and protein engineering available, our collection offers a foundation for genetic and molecular characterization of antifungal phenotypes, with potential for future exploitation. The scalable workflow can screen other yeast collections or adjust for different antifungal compounds.

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“… [17] According to reports, azole resistance in C. albicans detected in ICUs has grown over time. [18] There are additional contributing factors, such as differences in resistance rates between lower-middle-income and high-income countries, an inadequacy of infection control and prevention teams in low- and middle-income countries, and inadequacy in hand hygiene and isolation measures. In an increasingly connected world, the presence of MDR microorganisms poses a threat not only to developing countries but also to developed countries.…”

Section: Mdr Epidemiology In Icusmentioning

confidence: 99%

Antimicrobial stewardship in the intensive care unit

Türe

Güner

Alp

2023

Journal of Intensive Medicine

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Species Distribution and Antifungal Susceptibility Pattern of Candida Recovered from Intensive Care Unit Patients, Vietnam National Hospital of Burn (2017–2019)

Cited by 5 publications

References 38 publications

Azole non-susceptible C. tropicalis and polyclonal spread of C. albicans in Central Vietnam hospitals

Azole non-susceptible C. tropicalis and polyclonal spread of C. albicans in Central Vietnam hospitals

The antifungal capacity of a 681-membered collection of environmental yeast isolates

Antimicrobial stewardship in the intensive care unit

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