Species distribution and properties of staphylococci from canine dermatitis

Hauschild, Tomasz; Wójcik, Agnieszka

doi:10.1016/j.rvsc.2006.04.004

Cited by 42 publications

(36 citation statements)

References 25 publications

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“…In contrast, findings from 1995 demonstrated very effective activity of clindamycin against staphylococci (Dowling 1995). Interestingly, a similar study of S. intermedius isolated in Poland revealed that 31.57% of them were resistant to clindamycin (Hauschild and Wójcik 2007). None of these strains was resistant to gentamicin in contrast to our strains, 10% of which showed resistance to this antibiotic.…”

Section: Discussionsupporting

confidence: 42%

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Antibiotic resistance of canine Staphylococcus intermedius group (SIG) - practical implications

Chrobak¹,

Kizerwetter-Świda²,

Rzewuska³

et al. 2011

Polish Journal of Veterinary Sciences

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A total of 221 SIG strains were isolated from clinical samples of canine origin submitted to the Diagnostic Laboratory of the Division of Bacteriology and Molecular Biology at the Warsaw University of Life Sciences in Warsaw during the period 2006-2010. The aim of the study was to investigate the frequency of prevalence of methicillin-resistant SIG strains and to determine the MIC values of cephalotin, amoxicillin/clavulanic acid, ciprofloxacin, clindamycin, gentamicin, chloramphenicol, mupirocin for a collection of randomly selected 79 strains belonging to Staphylococcus intermedius group (SIG), including 23 mecA-positive and 56 mecA-negative strains. All isolates were identified as belonging to SIG based on their phenotypic properties and PCR amplification of S. intermedius-specific fragment of the 16S rRNA gene. The mecA gene was detected in 26 (12%) of 221 SIG strains. All tested mecA-negative SIG strains were susceptible to amoxicillin/clavulanic acid and cephalotin. One of the 56 mecA-negative SIG strains was resistant to ciprofloxacin, six (11%) to gentamicin. It was found that sixteen (29%) of 56 mecA-negative SIG strains were resistant to clindamycin. Most of the mecA-positive SIG strains were resistant to ciprofloxacin (96%), clindamycin (96%), and gentamicin (96%). Only one MRSIG strain was resistant to chloramphenicol. All examined mecA-positive SIG strains were found to be susceptible to mupirocin. Our results imply that staphylococcal multidrug resistance has become more prevalent, which could lead to difficulties in effective treatment. With some resistant strains the only therapeutic possibility are antimicrobial agents important in human medicine. New regulations for veterinary medicine concerning appropriate therapy of infections caused by multidrug-resistat staphylococci are needed.

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Section: Discussionsupporting

confidence: 42%

“…However, few studies of the antimicrobial susceptibility patterns of these isolates from dogs in Poland have been reported (Hauschild andWójcik 2007, Kizerwetter-Świda et al 2009). …”

Section: Introductionmentioning

confidence: 99%

Antibiotic resistance of canine Staphylococcus intermedius group (SIG) - practical implications

Chrobak¹,

Kizerwetter-Świda²,

Rzewuska³

et al. 2011

Polish Journal of Veterinary Sciences

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“…Many studies from various countries (Medleau et al 1986;Noble & Kent 1992;Kruse et al 1996;Stegemann et al 1996;Pellerin et al 1998;Scott et al 1998;Bloom & Rosser 2001;Holm et al 2002;Petersen et al 2002;Ganiere et al 2005;Hauschild & W ojcik 2007;Huerta et al 2011;Saridomichelakis et al 2002) have shown that the in vitro susceptibility rates of staphylococcal isolates from cases of pyoderma range from 65 to 94% and that this agent is clinically effective in 71 to 100% of affected dogs (Harvey et al 1993;Scott et al 1998;Littlewood et al 1999;Bloom & Rosser 2001). We have previously shown that, in healthy dogs, the oral dosage regimen of 11 mg/kg BW once daily has a better pharmacokinetical/ pharmacodynamical profile compared to 5.5 mg/kg BW orally twice daily (Saridomichelakis et al 2011).…”

Section: Introductionmentioning

confidence: 99%

Concentrations of clindamycin hydrochloride in homogenates of normal dog skin when administered at two oral dosage regimens

Saridomichelakis

Athanasiou

Chatzis

et al. 2013

Veterinary Quarterly

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Background: Clindamycin is frequently used for the treatment of bacterial pyoderma. Objective: To compare the pharmacokinetics of clindamycin in whole skin homogenates of normal dogs when administered orally at two dosage regimens (5.5 mg/kg BW, twice daily and 11 mg/kg BW, once daily). Animals and Methods: Skin biopsies were obtained from six laboratory beagles before, 3, 6 and 12 h after the first and the fifth dose of clindamycin at the former regimen, as well as before, 3, 6, 12 and 24 h after the first and third dose at the latter regimen. Tissue was homogenized and clindamycin concentrations were measured by reverse-phase high-performance liquid chromatography coupled with mass spectrometry. Results were analyzed using Student's t-test at a level of significance of 0.05. Results: Maximal concentration and area under the concentration-time curve, but not their relevant dose-normalized values, were higher at the dosage regimen of 11 mg/kg BW, once daily than at 5.5 mg/kg BW twice daily. Conclusions: The pharmacokinetic profile of clindamycin is at least equal, if not better, when this antimicrobial is administered at 11 mg/kg BW, once daily.

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“…However, the increasing prevalence of S. aureus infections and the emergence of a new species like S. schleiferi force the veterinary community to become more vigilant to prevent zoonosis (33,35). Other Staphylococcus species isolated from canine pyodermas and dermatitis were S. hyicus, S. epidermidis, S. xylosus, S. simulans, S. hominis (20,21), and S. chromogenes, S. sciuri, S. saprophyticus, S. capitis (15), respectively. S. intermedius (85.4%) and S. aureus (9.8%) isolates from the dogs with pyoderma constituted the main material of this study.…”

Section: Discussionmentioning

confidence: 99%

Molecular detection of exfoliative toxin in Staphylococcus intermedius isolates from dogs with pyoderma

MÜŞTAK¹

2013

Ankara Üniversitesi Veteriner Fakültesi Dergisi

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Summary:Staphylococcus aureus and S. intermedius are considered as the most significant species causing skin infections in dogs. The aim of this study was to develop a Polymerase Chain Reaction technique for the detection of siet (S. intermedius exfoliative toxin) gene encoding exfoliative toxin in S. intermedius and to investigate its presence in S. intermedius isolates from dogs with pyoderma. A total of 41 isolates (35 S. intermedius, 4 S. aureus, one S. capitis subsp. ureolytica and one S. chromogenes) from dogs with pyoderma were included in the study. Original primers specifically amplifying 145 bp of siet gene and 182 bp of agr (accessory gene regulator) gene locus of S. intermedius were designed in the study. agr gene was detected in all S. intermedius isolates, but not in other isolates. siet gene was detected in all S. intermedius isolates. siet Polymerase Chain Reaction assay was found to be specific since no amplifications were observed with siet negative S. intermedius and other bacterial control strains. Rapid and reliable detection of staphylococci causing skin lesions in dogs and their virulence markers like siet gene will provide important data for clinical practice to manage the disease more effectively by means of treatment and prevention.Key words: Canine pyoderma, exfoliative toxin, siet gene, Staphylococcus intermedius. Piyodermalı köpeklerden izole edilen Staphylococcus intermedius'larda eksfoliatif toksin varlığının moleküler olarak belirlenmesi

show abstract

Species distribution and properties of staphylococci from canine dermatitis

Cited by 42 publications

References 25 publications

Antibiotic resistance of canine Staphylococcus intermedius group (SIG) - practical implications

Antibiotic resistance of canine Staphylococcus intermedius group (SIG) - practical implications

Concentrations of clindamycin hydrochloride in homogenates of normal dog skin when administered at two oral dosage regimens

Molecular detection of exfoliative toxin in Staphylococcus intermedius isolates from dogs with pyoderma

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