Species distribution of coagulase-negative staphylococci isolated from clinical sources

Iwantscheff, Alexander; Kühnen, Ernst; Brandis, H.

doi:10.1016/s0176-6724(85)80096-1

Cited by 9 publications

(9 citation statements)

References 31 publications

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“…33,1989 on May 12, 2018 by guest http://aac.asm.org/ Downloaded from (5,7,9,12,15,22,24,25,27,28). Eighty percent of our isolates were from wounds, urine, and blood.…”

Section: (3)mentioning

confidence: 93%

“…With the exception of trimethoprim resistance determinants, similar genes were found among concurrently isolated multiply resistant S. epidermidis isolates from our hospitals. S. haemolyticus is a potentially important nosocomial species which readily acquires antimicrobial resistance genes and which shares, to some extent, in a common gene pool with S. epidermidis.Staphylococcus haemolyticus has been reported to represent an average of 10% of clinical coagulase-negative staphylococcal isolates (5,7,9,12,15,22,24,25,27,28). Many of these isolates have been found to be resistant to multiple antimicrobial agents.…”

mentioning

confidence: 99%

“…Staphylococcus haemolyticus has been reported to represent an average of 10% of clinical coagulase-negative staphylococcal isolates (5,7,9,12,15,22,24,25,27,28). Many of these isolates have been found to be resistant to multiple antimicrobial agents.…”

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confidence: 99%

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Antimicrobial resistance in nosocomial isolates of Staphylococcus haemolyticus

Froggatt

Johnston

Galetto

et al. 1989

Antimicrob Agents Chemother

169

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Staphylococcus haemolyticus is frequently cultured from hospitalized patients and is characterized by resistance to multiple antimicrobial agents. We found that S. haemolyticus represented 70 of 524 (13%) coagulase-negative staphylococcal isolates identified by the clinical microbiology laboratories of two hospitals over 2 months. S. haemolyticus isolates were recovered from wounds (44%), urine (26%), blood (10%), and other sources (20%). All S. haemolyticus isolates were tested for susceptibility to six antimicrobial agents; 77% were resistant to three or more agents, and 41% were resistant to five or six agents. In addition, among 47 multiply resistant isolates, high MICs (.6.25 ,ug/ml) of vancomycin (62% of isolates) and teicoplanin (91% of isolates) were found. DNA probes which were derived from S. epidermidis or S. aureus and which contained sequences associated with resistance to antimicrobial agents were used to detect specific genes in the total cellular and plasmid DNAs of 10 resistant S. haemolyticus isolates. Resistance gene probes and the numbers of resistant isolates hybridizing were as follows: methicillin, 10 of 10; gentamicin, 9 of 10; erythromycin, 7 of 10; and trimethoprim, 0 of 10. Genes for resistance to methicillin were found only in chromosomal locations, genes for resistance to gentamicin were found in both chromosomal and plasmid locations, and genes for resistance to erythromycin were found in plasmid locations only. With the exception of trimethoprim resistance determinants, similar genes were found among concurrently isolated multiply resistant S. epidermidis isolates from our hospitals. S. haemolyticus is a potentially important nosocomial species which readily acquires antimicrobial resistance genes and which shares, to some extent, in a common gene pool with S. epidermidis.Staphylococcus haemolyticus has been reported to represent an average of 10% of clinical coagulase-negative staphylococcal isolates (5,7,9,12,15,22,24,25,27,28). Many of these isolates have been found to be resistant to multiple antimicrobial agents. The importance of these organisms as human pathogens relative to that of other coagulase-negative staphylococci is uncertain, in part because species determinations of coagulase-negative staphylococci are not routinely done in most clinical microbiology laboratories. The mechanisms of antimicrobial resistance in S. haemolyticus and the extent to which it shares resistance genes with other staphylococci are also unknown. In a recent study of trimethoprim resistance among coagulase-negative staphylococci (6), we found that a specific trimethoprim resistance gene probe from a conjugative S. aureus plasmid hybridized with all trimethoprim-resistant S. epidermidis isolates but not with four S. haemolyticus isolates tested. The possibility of unique mechanisms of resistance in this poorly understood species led us to further investigate its characteristics.In the present study, we determined the proportion of S. haemolyticus isolates among clinical isolates of coagulasenega...

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“…33,1989 on May 12, 2018 by guest http://aac.asm.org/ Downloaded from (5,7,9,12,15,22,24,25,27,28). Eighty percent of our isolates were from wounds, urine, and blood.…”

Section: (3)mentioning

confidence: 93%

mentioning

confidence: 99%

See 1 more Smart Citation

Antimicrobial resistance in nosocomial isolates of Staphylococcus haemolyticus

Froggatt

Johnston

Galetto

et al. 1989

Antimicrob Agents Chemother

169

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“…This is most likely due to the overwhelming preponderance of S. epidermidis on the skin (88)(89)(90)(91)94); however, S. epidermidis may also possess virulence factors absent in other coagulase-negative staphylococci (9,26,27,53,55,56,98,163). S. saprophyticus appears to be a significant cause of urinary tract infections in young women, but has rarely been implicated in other types of infections (50,77,87,89 …”

Section: Microbiology Classificationmentioning

confidence: 99%

“…Newsl. 8: [37][38][39][40][41][42]1986), involve S. epidermidis as the most common etiologic agent (25,26,42,64,76,77,107,112,123,151), and are associated with implanted prosthetic devices such as intravenous catheters, prosthetic heart valves, prosthetic joints, or central nervous system shunts (3,8,9,19,20,23,30,62,75,81,82,97,120,129,141,154). Colonization of foreign bodies by coagulasenegative staphylococci frequently leads to serious local and systemic disease.…”

Section: Epidemiology and Clinical Significancementioning

confidence: 99%

Laboratory, clinical, and epidemiological aspects of coagulase-negative staphylococci

1988

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Coagulase-negative staphylococci, particularly Staphylococcus epidermidis, are increasingly important causes of nosocomial infection. Microbiologists and clinicians no longer can afford to disregard clinical isolates of coagulase-negative staphylococci as contaminants. Accurate species identification and antimicrobial susceptibility testing, in a clinically relevant time frame, are important aids in the diagnosis and management of serious coagulase-negative staphylococcal infections. Emphasis in the clinical laboratory should be placed on the routine identification of S. epidermidis and Staphylococcus saprophyticus, with identification of other species of coagulase-negative staphylococci as clinically indicated. The application of newer techniques, such as plasmid analysis and tests for slime production and adherence, contribute to our understanding of the epidemiology and pathogenesis of coagulase-negative staphylococci and may also be helpful in establishing the diagnosis of infection.

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Molecular diagnostics of clinically important staphylococci

2004

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Multilocus sequence typing 368 3.4 Innovative high-throughput molecular methods 369 3.4.1 Denaturing gradient gel electrophoresis 369 3.4.2 DNA microarrays 369 3.4.3 Fluorescence in situ hybridization with peptide nucleic acid probes 369 3.4.4 Matrix-assisted laser desorption/ionization time-of-flight mass spectrometry 370 4 Genes and probes used for diagnostics of staphylococci 370 4.1 Genes and sequences used in identification of staphylococcal species 370 4.2 Specific genes and sequences used in typing of S. aureus strains 370 4.3 Identification and typing of methicillin-resistant S. aureus strains 370 4.4 Glycopeptide-intermediate-resistant S. aureus 373 5 Conclusions 373 References 373

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Species distribution of coagulase-negative staphylococci isolated from clinical sources

Cited by 9 publications

References 31 publications

Antimicrobial resistance in nosocomial isolates of Staphylococcus haemolyticus

Antimicrobial resistance in nosocomial isolates of Staphylococcus haemolyticus

Laboratory, clinical, and epidemiological aspects of coagulase-negative staphylococci

Molecular diagnostics of clinically important staphylococci

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