Species Diversity of Micromycetes Associated with Epipactis helleborine and Epipactis purpurata (Orchidaceae, Neottieae) in Southwestern Poland

Ogórek, Rafał; Kurczaba, Klaudia; Łobas, Zbigniew; Żołubak, Elżbieta; Jakubska-Busse, Anna

doi:10.3390/d12050182

Cited by 7 publications

(3 citation statements)

References 75 publications

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“…In particular, strains of Exophiala radicis were found associated with roots both in Europe and in South America [91]. As concerns the presence of Zygomycota in the shared mycobiota, only Mucor moelleri and Absidia anomala were found in low abundances (0.23% and 0.04%, respectively) and they are both cosmopolitan saprotroph fungi inhabiting various environments [92,93].…”

Section: Discussionmentioning

confidence: 99%

The Mycobiota of High Altitude Pear Orchards Soil in Colombia

Nicola

Landínez-Torres

Zambuto

et al. 2021

Biology

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In Colombia, the cultivation of deciduous fruit trees such as pear is expanding for socio-economic reasons and is becoming more and more important for the local population. Since organized cultivation is slowly replacing sustenance cultivation, scientific information on the present agro-environment is needed to proceed in this change in an organic and environmentally friendly way. In particular, this study is an accurate description of the mycobiota present in the bulk soil of two different high altitude pear orchards in the Colombian Andes. The metabarcoding of soil samples allowed an in-depth analysis of the whole fungal community. The fungal assemblage was generally dominated by Ascomycota and secondly by Mortierellomycota. As observed in other studies in Colombia, the genus Mortierella was found to be especially abundant. The soil of the different pear orchards appeared to host quite different fungal communities according to the soil physico-chemical properties. The common mycobiota contained 35 fungal species, including several species of Mortierella, Humicola, Solicoccozyma and Exophiala. Moreover, most of the identified fungal species (79%) were recorded for the first time in Colombian soils, thus adding important information on soil biodiversity regarding both Colombia and pear orchards.

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Section: Discussionmentioning

confidence: 99%

The Mycobiota of High Altitude Pear Orchards Soil in Colombia

Nicola

Landínez-Torres

Zambuto

et al. 2021

Biology

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“…Plates were incubated in the dark at 15 and 20 ± 1 °C for 7–42 days. The fungi were identified according to available monographs [ 46 , 47 , 48 , 49 , 50 , 51 , 52 , 53 , 54 , 55 , 56 , 57 , 58 ]. Additionally, the phenotypes of some fungi were also compared with strains from R. Ogórek’s collection (Department of Mycology and Genetics, Institute of Genetics and Microbiology, University of Wrocław, Wrocław, Poland), which were identified using phenotypic and molecular studies, based on their internal transcribed spacer (ITS) region sequences deposited in the National Center for Biotechnology Information (NCBI, Bethesda, Rockville, MD, USA).…”

Section: Methodsmentioning

confidence: 99%

A Culture-Based ID of Micromycetes on the Wing Membranes of Greater Mouse-Eared Bats (Myotis myotis) from the “Nietoperek” Site (Poland)

Ogórek

Kurczaba

Cal

et al. 2020

Animals

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Bats play important functions in ecosystems and many of them are threatened with extinction. Thus, the monitoring of the health status and prevention of diseases seem to be important aspects of welfare and conservation of these mammals. The main goal of the study was the identification of culturable fungal species colonizing the wing membranes of female greater mouse-eared bat (Myotis myotis) during spring emergence from the “Nietoperek” underground hibernation site by the use of genetic and phenotypic analyses. The study site is situated in Western Poland (52°25′ N, 15°32′ E) and is ranked within the top 10 largest hibernation sites in the European Union. The number of hibernating bats in the winter exceeds 39,000 individuals of 12 species, with M. myotis being the most common one. The wing membranes of M. myotis were sampled using sterile swabs wetted in physiological saline (0.85% NaCl). Potato dextrose agar (PDA) plates were incubated in the dark at 8, 24 and 36 ± 1 °C for 3 up to 42 days. All fungi isolated from the surface of wing membranes were assigned to 17 distinct fungal isolates belonging to 17 fungal species. Penicillium chrysogenum was the most frequently isolated species. Some of these fungal species might have a pathogenic potential for bats and other mammals. However, taking into account habitat preferences and the life cycle of bats, it can be assumed that some fungi were accidentally obtained from the surface of vegetation during early spring activity. Moreover, Pseudogymnoascus destructans (Pd)—the causative agent of the White Nose Syndrome (WNS)—was not found during testing, despite it was found very often in M. myotis during previous studies in this same location.

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“…Grown fungal colonies were inoculated on PDA plates and crude cultures were obtained using single spore technique. The isolates of E. nigrum were identified according to available monographs [25,[46][47][48]. Additionally, the phenotypes of E. nigrum isolates were also compared with strains from R. Ogórek's, which were identified using phenotypic and molecular studies, and their ITS region sequences deposited in the NCBI (Bethesda, Rockville, MD, USA).…”

Section: Assessing Host Specialization In E Nigrum Strainmentioning

confidence: 99%

Plant–Fungal Interactions: A Case Study of Epicoccoum nigrum Link

Ogórek

Przywara

Piecuch

et al. 2020

Plants

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Epicoccum nigrum Link is a cosmopolitan species, and it has been described as both an in vitro and in vivo antagonist of many fungal pathogens of plants. However, there are no clear reports about the interactions between E. nigrum and various plant species, and about the effects of culture filtrates produced by this fungus on plants. Therefore, we assessed the interactions between E. nigrum and different plant species, such as sugar beet (Beta vulgaris L. ssp. vulgaris), spring wheat (Triticum aestivum L.), red clover (Trifolium pratense L.), and winter oilseed rape (Brassica napus L.). Additionally, we evaluated the effect of E. nigrum culture filtrates on garden cress (Lepidium sativum L.). Our study showed that the E. nigrum strains varied in terms of the color of excreted culture filtrates and showed different interactions with garden cress. Overall, fungal strains only affected adversely the sprout length in a significant way and, partially, the growth of the tested plant. In addition, we confirmed the suitability of the garden cress as a test plant in in vitro toxicological tests. Most strains of E. nigrum (61.1%) secreted enzymes expected to participate mainly in the later stages of the infection (amylases and proteases) and not those expected to operate in the early phases of host penetration (cellulases and pectinases) that were secreted by 33.3% of fungal strains. The group of pectinolytic enzymes represented the catalysts with the highest activity. Host specialization tests showed that E. nigrum was mainly re-isolated from the plant surface and the number of infected seedlings as well as the disease index depended on a studied plant species, with sugar beet and red clover being most sensitive to infection. In turn, the lowest value of the disease index caused by E. nigrum strains was recorded for spring wheat and winter oilseed rape. Overall, statistically significant differences in the growth of plant seedlings during the host specialization test were noted only for sugar beet and red clover seedlings. The seedlings of plants in the control group (without fungal inoculum) exhibited an increased length compared to those treated with E. nigrum inoculum. Our studies also showed that E. nigrum is probably a facultative saprotroph of plants and it may winter on red clover, which is presumably its main reservoirs, among the species considered.

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Species Diversity of Micromycetes Associated with Epipactis helleborine and Epipactis purpurata (Orchidaceae, Neottieae) in Southwestern Poland

Cited by 7 publications

References 75 publications

The Mycobiota of High Altitude Pear Orchards Soil in Colombia

The Mycobiota of High Altitude Pear Orchards Soil in Colombia

A Culture-Based ID of Micromycetes on the Wing Membranes of Greater Mouse-Eared Bats (Myotis myotis) from the “Nietoperek” Site (Poland)

Plant–Fungal Interactions: A Case Study of Epicoccoum nigrum Link

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