Species Identification and Sex Determination of the Genus Nepenthes (Nepenthaceae)

Mokkamul, Piya; Chaveerach, Arunrat; Sudmoon, Runglawan; Tanee, Tawatchai

doi:10.3923/pjbs.2007.561.567

Cited by 10 publications

(3 citation statements)

References 10 publications

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“…Random Amplified Polymorphic DNA can detect loci in several areas of a genome at the same time. Although RAPDs are considered to be dominant, meaning that do not allow to distinguish whether the amplified DNA segment is heterozygous or homozygous at a particular locus, they have has been widely used for taxonomic and phylogenetic studies (Bartish, Rumpunen & Nybom, 2000;Mokkamul, Chaveerach, Sudmoon & Tanee, 2007;Verma, Karihaloo, Tiwari, Magotra & Koul, 2007), for species discrimination (Chaveerach, Tanomtong, Sudmoon & Tanee, 2006;Zhang et al, 2007) and for phyleogeographic studies (Wu et al, 2006). However, to achieve reproducible profiles a strictly consistent reaction conditions is needed during the run of RAPDs methodology.…”

Section: Not Sequence-depended Dna Markersmentioning

confidence: 99%

Detection of Authentication of Meat Products by Low Cost Closed-tube Molecular Methods

Laliotis,

Batrinou,

Giannakourou

et al. 2023

JFR

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Nowadays adulteration of meat products, especially of ground meat products which form an easy case scenario for implementing adulteration practices due to their structure and texture, emerges a critical issue of raised concern threatening fair trade, food quality and consumers’ health and protection. Food authentication testing is the tool to address this kind of fraud. There is several analytical methodologies applied for meat authentication targeting at different biomarkers and using a variety of analytical techniques. However, the applied methodologies should exhibit suitable performance characteristics such as reliability, sensitivity, reproducibility and availability in order to be fit for purpose. During the last 20 years, amplification tests have emerged as an important diagnostic tool, not only for clinical applications, but also for food quality and safety. It was urgent to develop molecular techniques fast and sensitive. The introduction of new DNA technologies has facilitated the ease and accuracy of of methods for fraud detection. The closed-tube methods of Loop-mediated isothermal amplification (LAMP) and Gold Nanoparticles linked with oligonucleotides used as molecular probes are well known for their robust and highly sensitive and specific amplification of target DNA. Moreover, these techniques are rapid, low-cost diagnostics and available on site. This review provides a comprehensive overview of the molecular methods developed that can be applied for investigating ground meat adul-teration and focuses on the advantages of the rapid closed tube methods that can yield color results interpreted with the naked eye. The application of such time- and cost-effective molecular tools in the food market is proposed to provide a first-level filter for meat adulterated products, serving as a complementary tool to the more in-depth -omics approach.

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Section: Not Sequence-depended Dna Markersmentioning

confidence: 99%

Detection of Authentication of Meat Products by Low Cost Closed-tube Molecular Methods

Laliotis,

Batrinou,

Giannakourou

et al. 2023

JFR

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“…Nepenthaceae is represented by a single genus Nepenthes which is popularly known as tropical pitcher plant (Mokkamul et al 2007). Indo-Malaysia is considered as the center of evolution of the genus Nepenthes.…”

Section: Introductionmentioning

confidence: 99%

PCR based molecular characterization of Nepenthes khasiana Hook. f.—pitcher plant

Bhau

Medhi

Sarkar

et al. 2009

Genet Resour Crop Evol

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Nepenthes khasiana Hook. f. belonging to monotypic family Nepenthaceae is a rare, endangered, dioecious member of the carnivorous plant found in North-East India. The plant is endemic to the Indian state of Meghalaya and is distributed throughout the state from West Khasi hills to East Khasi hills, Jaintia hills and East, West to South Garo hills from 1,000 to ca. 1,500 m altitude. Multi-locus analysis using PCR based Random Amplified Polymorphic DNA (RAPD) and Inter Simple Sequence Repeats (ISSR) markers were used for the first time to assess the genetic diversities of N. khasiana Hook. f. collected from different parts of Meghalaya. It was observed that RAPD analysis showed more polymorphism than ISSR fingerprinting in revealing genetic polymorphism in N. khasiana Hook. f. The result of cluster analysis by using UPGMA method showed that the groups based on pooled RAPD-ISSR genetic similarity were more similar than the groups based on RAPD. Furthermore, genetic similarity reveals variability within the population at Jarain of Jaintia hills, while between populations the Baghmara region differs from the others with at least 40% dissimilarity. The results show a broad range of genetic diversity within the populations of N. khasiana Hook. f.

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“…The simplicity, efficiency, and relative ease of performing RAPD techniques, without sequence information, have led to their expanded use for taxonomic and systemic analysis and phylogenetic studies of plants, species differentiation, and phylogeographic variation, as well as for studying breeding and genetic relationships [10][11][12][13][14][15][16][17][18][19][20]. RAPD techniques have recently been used in genetic diversity studies of a wide range of tree plants [21,22].…”

Section: Introductionmentioning

confidence: 99%

Genetic diversity of <i>Persea bombycina</i> from goalpara district of Assam, India

Rahman¹,

Tanti²,

Sarma³

et al. 2012

ABB

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Assam of the northeastern region of India is unique in terms of its rich biodiversity and multiple ethnicity of its people. The impact of the resultant socio-religiocultural diversity is also reflected in the diverse traditional ways of silkworm farming. We report the genetic diversity of Persea bombycina "Som" from different locations of Goalpara district of Assam, India, where random amplified polymorphic DNA (RAPD) marker was used in this study. RAPD analyses of ten genotypes of "Som" from Goalpara district of Assam, India with B19 RAPD primer generated 86 bands, showing an average of 8.6 bands per sample and 30.2% (26 bands) of these were polymorphic. The number of bands per accession ranged from 5 to 10 with a mean of 8.6 and the size range of the amplified bands was 250 -6000 bp. In a UPGMA phenetic dendrogram based on Jaccard's coefficient, the P. bombycina accessions showed a high level of genetic variation, as indicated by genetic similarity and revealed 10 "Som" genotypes in to three major clusters. This study may be useful in identifying diverse genetic stocks of P. bombycina, which may then be conserved on a priority basis. The RAPD primer used in this study was able to distinguish all the 10 genotypes of "Som" plants, which can be used to assess genetic diversity.

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Species Identification and Sex Determination of the Genus Nepenthes (Nepenthaceae)

Cited by 10 publications

References 10 publications

Detection of Authentication of Meat Products by Low Cost Closed-tube Molecular Methods

Detection of Authentication of Meat Products by Low Cost Closed-tube Molecular Methods

PCR based molecular characterization of Nepenthes khasiana Hook. f.—pitcher plant

Genetic diversity of <i>Persea bombycina</i> from goalpara district of Assam, India

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Species Identification and Sex Determination of the Genus Nepenthes (Nepenthaceae)

Cited by 10 publications

References 10 publications

Detection of Authentication of Meat Products by Low Cost Closed-tube Molecular Methods

Detection of Authentication of Meat Products by Low Cost Closed-tube Molecular Methods

PCR based molecular characterization of Nepenthes khasiana Hook. f.—pitcher plant

Genetic diversity of &lt;i&gt;Persea bombycina&lt;/i&gt; from goalpara district of Assam, India

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Genetic diversity of <i>Persea bombycina</i> from goalpara district of Assam, India