Species identification, antibiotic sensitivity and slime production of coagulase-negative staphylococci isolated from clinical specimens

Deighton, Margaret; Franklin, John; Spicer, W. John; Balkau, Beverley

doi:10.1017/s0950268800029265

Cited by 37 publications

(17 citation statements)

References 48 publications

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“…This study showed that S. epidermidis was the most common CoNS species comprising 43% of all isolates similar to that of other reports [11][12][13][14][15][16] . CoNS was reported as an important cause of blood stream infections and the second most common source after wound swabs in a hospital in Lebanon and Kuwait [17][18] .…”

Section: Discussionsupporting

confidence: 91%

Prevalence and antimicrobial susceptibility pattern of Coagulase-negative Staphylococcus

Begum¹,

Anbumani²,

Kalyani³

et al. 2011

IJMEDPH

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Section: Discussionsupporting

confidence: 91%

Prevalence and antimicrobial susceptibility pattern of Coagulase-negative Staphylococcus

Begum¹,

Anbumani²,

Kalyani³

et al. 2011

IJMEDPH

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“…Tube method is a qualitative method for the detection of biofilm production as it was difficult to differentiate between moderate and weak biofilm producers [15]. The results were correlated with previous studies [12,13].…”

Section: Discussionsupporting

confidence: 83%

“…In terms of biofilm presence or absence, 18 (28.12%) isolates were non-biofilm producers while 46 (71.87%) isolates were positive for biofilm production. The results demonstrated that tube method is easy to perform while interpretation of the results is quite complicated particularly with the novice observer, so it cannot be recommended as general screening test for determination of biofilm production [16,15]. In addition, micro-plate assay showed that 56.25% of the isolates were positive for biofilm production.…”

Section: Discussionmentioning

confidence: 99%

Detection of Biofilm Production by Staphylococcus Aureus and in-Vitro Inhibition of Biofilm Using Serum Antibodies

Raza¹,

Qamar²,

Manzoor³

et al. 2017

Matrix sci. medica

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The biofilm producing ability of Staphylococcus aureus has been implicated for development of different diseases in animals and humans. The presence of biofilms makes the bacterial population resistant to a wide range of antibiotics. Biofilms are also antigenic structures of the bacterial population. Therefore, the present study was planned to evaluate the biofilm-producing ability of S. aureus and in-vitro inhibition of biofilm production using serum samples from rabbits immunized with biofilm-producing S. aureus bacterin-toxoid. A total of 64 S. aureus isolates were harvested from lactating cows and buffaloes suffering from clinical and sub-clinical mastitis. The biofilm-producing ability of S. aureus isolates was determined by tube method and micro-plate assays. The rabbits were vaccinated using strong biofilm producing S. aureus bacterin-toxoid. The ability of serum antibodies to inhibit in-vitro biofilm production was assessed by performing a biofilm inhibition assay. The results showed that 71.87% and 56.25% of the isolates were positive for biofilm production in tube method and micro-plate assays, respectively. The serum samples collected at days 30, 45 and 60 post-immunization completely inhibited the biofilm production by S. aureus. Our results suggested that both micro-plate assay and tube method can be used for determination of biofilm production, however micro-plate assay is quantitative, more accurate and reliable. The study also concluded that the antibodies produced against biofilm producing S. aureusbacterin-toxoid seems helpful to avert the attachment of bacteria, which may further implicate the better control of chronic infections.

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“…Sixteen patients (73%) were colonized by several different S. haemolyticus strains which were isolated from all body sites predominantly from the groin. A lower prevalence of S. haemolyticus has been reported in some studies (West et al 1986;Eisenberg et al 1987) however Deighton et al (1988) found it to be a common skin colonizer.…”

Section: Disctusstonmentioning

confidence: 80%

Prevalence of peritonitis-associated coagulase-negative staphylococci on the skin of continuous ambulatory peritoneal dialysis patients

et al. 1989

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SUMNIMARYThe predominance of coagulase-negative staphylococci as normal skin flora is thought to be a factor in their association with episodes of peritoinitis in patients undergoing continuous ambulatory peritoneal dialysis. We investigated the prevalence of peritonitis-associated strains on the skin of 28 patients undergoing peritoneal dialysis. Coagulase-negative staphylococci were the most frequently isolated organisms, comprising 47 % of peritoneal dialysis fluid isolates and( 59 % of body site isolates. A total of 142 coagulase-negative staphylococci were speciated, tested for their antimicrobial sensitivity and slime production, an(d identified by phage typing and plasmid-profile analysis. Staphylococctis epidernlidis was the most commonly identified species from both peritoneal dialysis fluid (73 %) and body sites (53 %). Multiple antibiotic resistance was common, and the greater proportion of isolates were resistant to methicillin; 63 6 % of peritoneal dialysis fluid isolates and 61-7 % of body-site isolates. S. haermolyticuus isolates were significantly more resistant to methicillin than other species. By phage typinig all( plasmid-profile analysis it was shown that peritonitis was rarely caused by skinl colonizing strains. In only 3 of 14 patients were peritonitis-associated strains isolated as skin colonizers, and no patients developed peritonitis due to organisms previously isolated as skin colonizers.

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Species identification, antibiotic sensitivity and slime production of coagulase-negative staphylococci isolated from clinical specimens

Cited by 37 publications

References 48 publications

Prevalence and antimicrobial susceptibility pattern of Coagulase-negative Staphylococcus

Prevalence and antimicrobial susceptibility pattern of Coagulase-negative Staphylococcus

Detection of Biofilm Production by Staphylococcus Aureus and in-Vitro Inhibition of Biofilm Using Serum Antibodies

Prevalence of peritonitis-associated coagulase-negative staphylococci on the skin of continuous ambulatory peritoneal dialysis patients

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