Species-level identification of staphylococci isolated from bovine mastitis in Brazil using partial 16S rRNA sequencing

Lange, C. C.; Brito, Maria Aparecida Vasconcelos Paiva; Reis, Danièle; Machado, M. A.; Guimarães, Alexandre Silva; Azevedo, Ana Luisa Sousa; Salles, Érica B.; Alvim, Mariana C.T.; Silva, Fabiana S.; Meurer, Igor Rosa

doi:10.1016/j.vetmic.2015.01.024

Cited by 51 publications

(52 citation statements)

References 21 publications

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“…The coa gene amplification also showed that the minority of the isolates belonged to CNS; which are capable of causing opportunistic mastitis (Moon et al, 2007). Lange et al (2015) reported S. chromogenes at a frequency of 38.5%, which highlights the importance of coagulase-negative strains; however, in this study, the detection of CNS was low. S. xylosus, coagulase-negative, were also detected, within this species there are strains that can potentially be hazardous, and they are related to animal opportunistic infections (Dordet-Frisoni et al, 2007).…”

Section: Discussionmentioning

confidence: 52%

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Antibiotic resistance and molecular characterization of Staphylococcus species from mastitic milk

Rodrigues¹,

Silva²,

Trevilin³

et al. 2017

Afr. J. Microbiol. Res.

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Species within the Staphylococcus genus are important mastitis pathogens. Studies to describe virulence and antibiotic resistance as well as rapid techniques that permit analyses strains are needed. The aims were to identify and characterize Staphylococcus spp. isolated from mastitic milk, and to optimize multiplex polymerase chain reactions (PCR). Staphylococci previously isolated from milk of dairy cows with subclinical mastitis were analyzed. PCR was completed to amplify nuc, sodA, spa, agr locus, virulence factors, and antibiotic resistance gene s. DNA sequencing of sodA and spa genes was performed and antibiograms were carried out on all isolates. In a group of 49 staphylococci, S. aureus was the most prevalent, followed by S. hyicus, S. xylosus, S. chromogenes. Following optimization of multiplex PCR, virulence factor genes were identified in the majority of isolates. The enterotoxin genes, seh and selx were highlighted. All hemolysin genes were detected in 28.6% of isolates. Antibiotic resistance was evaluated and the majority of isolate s (69.4%) were resistant to penicillin. Among the genes encoding antibiotic resistance , mecA was identified, while two methicillin-resistant S. aureus were typed as spa type 605, agr type II, and one identified as SCCmec type IVa. The types t605 and agr II were detected in the majority of S. aureus assessed. The findings emphasize d the importance of preventing Staphylococcus infection in dairy cows. Effective dairy herd management and information on milk quality are essential to prevent mastitis pathogens.

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Section: Discussionmentioning

confidence: 52%

“…frequency of this pathogen in Brazil (Silva et al, 2013;Lange et al, 2015). Giannechini et al (2002) also detected high frequency of S. aureus, and low frequency of S. hyicus coagulase-positive among isolates from subclinical mastitis cases.…”

Section: Discussionmentioning

confidence: 97%

Antibiotic resistance and molecular characterization of Staphylococcus species from mastitic milk

Rodrigues¹,

Silva²,

Trevilin³

et al. 2017

Afr. J. Microbiol. Res.

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“…S. chromogenes has been isolated most commonly in almost all studies but we couldn't isolate it in our study (Lange et al 2015).…”

Section: Discussion:-mentioning

confidence: 72%

“…In a recent study of MRSA isolated from milk samples from mastitic cows, 100% of isolates were resistant to clindamycin, chloramphenicol, erythromycin, and gentamicin (Lee et al 2015). In another study in Switzerland, two MRSA isolates from 142 milk samples from from mastitic cows were found resistant to all beta-lactam antibiotics (Haran et al 2012b;Lange et al 2015), while Vanderhaeghen et al reported resistance to tetracycline, with frequent resistance to macrolides, lincosamide, and aminoglycosides, in MRSA from milk from mastitic cows (Bardiau et al 2013). …”

Section: Discussion:-mentioning

confidence: 99%

Isolation and Molecular Identification of Staphylococcus Spp. From Bovine Mastitis Milk Samples.

Patel¹,

Joshi²,

Kunjadiya³

2017

IJAR

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“…Estudos recentes demonstram que a sensibilidade da PCR supera os métodos de cultura e permite a identificação do patógeno com alta especificidade e em poucas horas (Cantekin et al 2015). A maioria dos isolados SCP pertencem a espécie S. aureus (Krewer et al 2014) fato que também foi observado em nosso estudo onde o 69,2% dos isolados SCP foram identificados como S. aureus, mas também existem isolados de S. aureus negativos à prova de coagulase em tubo (Lange et al 2015).…”

Section: Discussionunclassified

Frequência de genes codificadores de toxinas em Staphylococcus aureus isolados de leite de tanques expansão comunitários

et al. 2017

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RESUMO.-A capacidade de produção de toxinas pelo Staphylococcus aureus no leite e produtos derivados está relacionado com surtos de intoxicação alimentar. Objetivou-se nesta pesquisa, estudar a ocorrência de genes que codificam para enterotoxinas estafilocócicas (sea, seb, sed, seg, seh e sei) e toxinas α e β hemolítica (hla e hlb) em S. aureus isolados de 53 amostras de leite de tanques expansão comunitários no Estado de Alagoas, Brasil. Foram identificados 27 isolados (50,94%) como S. aureus pela amplificação do gene nuc. 13/27 isolados (48,1%) foram positivos para pelo menos um gene das enterotoxinas estudadas, sendo as frequências dos genes sea 33,3%, seh 18,5%, sei 11,1% e sed 7,4%; não entanto não foram identificados os genes seb e seg nestas bactérias. Para as toxinas hemolíticas, 51,9% dos isolados portavam ambos genes (hla e hlb), sendo a frequência para o gene hla de 81,5% e para o gene hlb de 51,9%. A frequência de genes das toxinas avaliadas é alta o que constitui um risco potencial para a saúde pública em especial, as enterotoxinas por serem termoestáveis e estarem asssociados com surtos de intoxicação alimentar. The capacity of toxin production by Staphylococcus aureus in milk and dairy products is associated with food poisoning outbreaks. The objective of this research was to study the frequency of genes encoding staphylococcal enterotoxin (sea, seb, sed, seg, seh and sei) and α and β hemolytic toxins (hla and hlb) in S. aureus isolates from 53 milk samples from community tanks in the State of Alagoas, Brazil. Twenty-seven isolates (50.94%) were identified as S. aureus by nuc gene amplification; 13/27 isolates (48.1%) were positive for at least one gene of the studied enterotoxins and the frequency of genes sea was 33.3%, seh 18.5%, sei 11.1% and sed 7.4%; the seb and sec genes have not been identified in the bacteria. For the hemolytic toxins, 51.9% of isolates harbored both genes (hla and hlb), the frequency of hla gene was 81.5% and 51.9% for the hlb gene. The evaluated toxin-encoding gene frequency is high and constitutes a potential risk for public health, especially staphylococcal enterotoxin genes; because they are heat-stable enterotoxins and have been associated with food poisoning.

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Species-level identification of staphylococci isolated from bovine mastitis in Brazil using partial 16S rRNA sequencing

Cited by 51 publications

References 21 publications

Antibiotic resistance and molecular characterization of Staphylococcus species from mastitic milk

Antibiotic resistance and molecular characterization of Staphylococcus species from mastitic milk

Isolation and Molecular Identification of Staphylococcus Spp. From Bovine Mastitis Milk Samples.

Frequência de genes codificadores de toxinas em Staphylococcus aureus isolados de leite de tanques expansão comunitários

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