Species-selective determination of selenium compounds in biological materials (Technical Report)

Łobiński, Ryszard; Edmonds, John S.; Suzuki, Kazuo; Uden, Peter C.

doi:10.1351/pac200072030447

Cited by 157 publications

(81 citation statements)

References 78 publications

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“…[4][5][6] Selenoproteins contain selenium in the form of selenocysteinyl residues; proteins that contain this element in the form of selenomethionyl residues are not formally classified as selenoproteins. Analytical techniques for the determination of selenium species have been reviewed, [7][8][9][10] but many methods have only been applied to a few commercially available standards. Table 1 shows some selenium species that have attracted nutritional, clinical and bioanalytical attention.…”

Section: Introductionmentioning

confidence: 99%

Element selective characterization of stability and reactivity of selenium species in selenized yeast

Uden

Boakye

Kahakachchi

et al. 2004

J. Anal. At. Spectrom.

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The concerted application of element specific atomic spectral detection for chromatographic eluent monitoring allows previously unexploited qualitative and quantitative analytical concepts to be developed for the determination of selenium species. Selenium speciation is vital in order to better understand its metabolism and biological significance in clinical chemistry, biology, toxicology, and nutrition. Fluoroacid ion pair HPLC with ICP-MS detection and GC derivatization with atomic emission detection (AED) together aid analysis and elucidation of reaction pathways of selenium compounds in high selenium enriched yeast, as used widely in nutritional and clinical cancer preventative studies. Comparisons between currently produced and archived selenized yeasts show major differences in speciation. The formation of selenomethionine selenoxide and the identification of Se-S bonded S-(selenomethyl)-cysteine in archived nutritional yeast may be important for short and long term stability and nutritional activity studies.

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Section: Introductionmentioning

confidence: 99%

Element selective characterization of stability and reactivity of selenium species in selenized yeast

Uden

Boakye

Kahakachchi

et al. 2004

J. Anal. At. Spectrom.

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“…These selonoproteins or Se-containing proteins are efficiently fractionated by gel electrophoresis. Size-exclusion liquid chromatography is the fractionation method mostly used in combination with nonradioactive detection methods like ICP-MS, but with the limitation of the low chromatographic resolution typical for size-exclusion [9]. In a previous paper, we reported the use of gel electrophoresis for the fractionation of proteins in combination with electrothermal vaporization-ICP-MS for the detection of Se, with the advantages of a higher resolution than obtainable with size-exclusion and the quantitative aspect of the method [10].…”

Section: Introductionmentioning

confidence: 99%

Detection of metals in proteins by means of polyacrylamide gel electrophoresis and laser ablation‐inductively coupled plasma‐mass spectrometry: Application to selenium

et al. 2003

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The capabilities of laser ablation-inductively coupled plasma-mass spectrometry for the detection of trace elements in a gel after gel electrophoresis were systematically studied. Figures of merit, such as limit of detection, linearity, and repeatability, were evaluated for various elements (Li, V, Cr, Mn, Ni, Cu, Zn, As, Se, Mo, Pd, Ag, Cd, Pt, Tl, Pb). Two ablation strategies were followed: single hole drilling, relevant for ablation of spots after two-dimensional (2-D) separations, and ablation with translation, i.e., on a line, relevant for one-dimensional (1-D) separations. This technique was applied to the detection of selenoproteins in red blood cells extracts after a 1-D separation (sodium dodecyl sulfate-polyacrylamide gel electrophoresis) and the detection of selenium-containing proteins in yeast after 2-D electrophoresis (2-DE). The detection procedure was further improved by using the dynamic reaction cell technology, which allowed the removal of the Ar 2 interference and hence the use of the most abundant Se isotope, 80 Se. Reaction gases were compared (methane, carbon monoxide, ammonia, oxygen and the combination of argon (collision gas) and hydrogen (reaction gas)). In each instance, the reaction cell parameters were optimized in order to obtain the lowest detection limit for Se (as 80 Se 16 O 1 with O 2 as the reaction gas). Carbon monoxide was found to offer the best performance. The detection limit with the use of DRC and He as transport gas was 0.07 mg Se g 21 gel with single hole drilling and 0.15 mg Se g 21 gel for ablation with translation.

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“…The HPLC͞inductively coupled argon plasma massspectrometry (ICP-MS) and HPLC͞electrospray ionization tandem mass-spectrometry (ESI-MS͞MS) methods have been used efficiently for the speciation of bio-trace elements (15,16), and our recent work indicated that the major urinary metabolite (hepatic metabolite B) is a selenosugar, Se-methyl-N-acetylselenohexosamine (17). In the present study, we determined the chemical structures of two selenosugars and proposed their biological roles as intermediates in the metabolism of Se leading to urinary excretion.…”

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confidence: 99%

Selenosugars are key and urinary metabolites for selenium excretion within the required to low-toxic range

Kobayashi

Ogra²,

Ishiwata³

et al. 2002

Proc. Natl. Acad. Sci. U.S.A.

274

225

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Essential micronutrient selenium is excreted into the urine and͞or expired after being transformed to methylated metabolites. Monomethylated selenium is excreted into the urine in response to a supply within the required to low-toxic range, whereas tri-and dimethylated selenium increase with excessive supply at a toxic dose. Here we show that the major urinary selenium metabolite within the required to low-toxic range is a selenosugar. The structure of 1␤-methylseleno-N-acetyl-D-galactosamine was deduced from the spectroscopic data and confirmed by chemical synthesis. This metabolite was also detected in the liver, and an additional metabolite increased with inhibition of methylation. The latter metabolite was again a selenosugar conjugated with glutathione instead of a methyl group and was assumed to be a precursor for methylation to the former metabolite. A metabolic pathway for the urinary excretion of selenium, i.e., from the glutathione-S-conjugated selenosugar to the methylated one, was proposed. Urinary monomethylated (selenosugar) and trimethylated selenium can be used as specific indices that increase within the required to low-toxic range and with a distinct toxic dose, respectively.

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Species-selective determination of selenium compounds in biological materials (Technical Report)

Cited by 157 publications

References 78 publications

Element selective characterization of stability and reactivity of selenium species in selenized yeast

Element selective characterization of stability and reactivity of selenium species in selenized yeast

Detection of metals in proteins by means of polyacrylamide gel electrophoresis and laser ablation‐inductively coupled plasma‐mass spectrometry: Application to selenium

Selenosugars are key and urinary metabolites for selenium excretion within the required to low-toxic range

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