Species-Specific Cutaneous Biotransformation of the Pesticide Propoxur during Percutaneous Absorption in Vitro

Sandt, J.J.M. van de; Rutten, A.A.J.J.L.; Ommen, Ben van

doi:10.1006/taap.1993.1231

Cited by 30 publications

(14 citation statements)

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“…Prior to the start of the experiment, integrity of the skin preparations was assessed by determining the permeability coeYcient (K p ) of tritiated water, as described by van de Sandt et al (1993van de Sandt et al ( , 2000, using a cut-oV value of 4.0 £ 10 ¡3 cm/h. Along side with tritiated water, [ 14 C]propoxur, a pesticide with MW 209, log P OW 1.5, was used as marker of skin permeability.…”

Section: In Vitro Skin Permeability Assaymentioning

confidence: 99%

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Effects of single and repeated exposure to biocidal active substances on the barrier function of the skin in vitro

Buist¹,

Sandt²,

Burgsteden³

et al. 2005

Regulatory Toxicology and Pharmacology

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Section: In Vitro Skin Permeability Assaymentioning

confidence: 99%

“…The assay was performed in static diVusion cells using cryopreserved human abdominal skin, as previously described by van de Sandt et al (1993van de Sandt et al ( , 2000. The skin originated from seven female donors, aged 31-57 years (average 39).…”

Section: In Vitro Skin Permeability Assaymentioning

confidence: 99%

Effects of single and repeated exposure to biocidal active substances on the barrier function of the skin in vitro

Buist¹,

Sandt²,

Burgsteden³

et al. 2005

Regulatory Toxicology and Pharmacology

View full text Add to dashboard Cite

“…The assay was performed in static diffusion cells using cryopreserved human abdominal skin, as previously described by van de Sandt et al (1993. The skin originated from seven female donors, aged 31-57 years (average 39).…”

Section: In Vitro Skin Permeability Assaymentioning

confidence: 99%

“…Prior to the start of the experiment, integrity of the skin preparations was assessed by determining the permeability coefficient (k p ) of tritiated water, as described by van de Sandt et al (1993, using a cut-off value of 4.0 x 10 -3 cm/h. Alongside with tritiated water, Skin permeability may vary considerably between donors or due to differences in solvents.…”

Section: In Vitro Skin Permeability Assaymentioning

confidence: 99%

“…The receptor fluid (total volume 1.2 mL) consisted of a physiological salt solution (0.9% NaCl w/v) containing 0.01% sodium azide and 6% polyoxyethylene (20) oleyl ether, the latter having been added to ensure also lipophilic test substances would be readily soluble in it. Prior to the start of the experiment, integrity of the epidermal membranes was assessed by determining the permeability coefficient (k p ) of tritiated water, as described by van de Sandt et al (1993. Epidermal membranes with a k p for tritiated water of less than 3.0 x 10 -3 cm/h were used in the subsequent experiments.…”

Section: In Vitro Dermal Absorption Experimentsmentioning

confidence: 99%

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Dermal absorption and toxicological risk assessment : pitfalls and promises

Buist¹

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Comparison of the metabolism of 10 chemicals in human and pig skin explants

Géniès

Jamin

Debrauwer

et al. 2018

J of Applied Toxicology

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Skin metabolism is important to consider when assessing local toxicity and/or penetration of chemicals and their metabolites. If human skin supply is limited, pig skin can be used as an alternative. To identify any species differences, we have investigated the metabolism of 10 chemicals in a pig and human skin explant model. Phase I metabolic pathways in skin from both species included those known to occur via cytochrome P450s, esterases, alcohol dehydrogenases and aldehyde dehydrogenases. Common Phase II pathways were glucuronidation and sulfation but other conjugation pathways were also identified. Chemicals not metabolized by pig skin (caffeine, IQ and 4‐chloroaniline) were also not metabolized by human skin. Six chemicals metabolized by pig skin were metabolized to a similar extent (percentage parent remaining) by human skin. Human skin metabolites were also detected in pig skin incubations, except for one unidentified minor vanillin metabolite. Three cinnamyl alcohol metabolites were unique to pig skin but represented minor metabolites. There were notable species differences in the relative amounts of common metabolites. The difference in the abundance of the sulfate conjugates of resorcinol and 4‐amino‐3‐nitrophenol was in accordance with the known lack of aryl sulfotransferase activity in pigs. In conclusion, while qualitative comparisons of metabolic profiles were consistent between pig and human skin, there were some quantitative differences in the percentage of metabolites formed. This preliminary assessment suggests that pig skin is metabolically competent and could be a useful tool for evaluating potential first‐pass metabolism before testing in human‐derived tissues.

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Species-Specific Cutaneous Biotransformation of the Pesticide Propoxur during Percutaneous Absorption in Vitro

Cited by 30 publications

References 0 publications

Effects of single and repeated exposure to biocidal active substances on the barrier function of the skin in vitro

Effects of single and repeated exposure to biocidal active substances on the barrier function of the skin in vitro

Dermal absorption and toxicological risk assessment : pitfalls and promises

Comparison of the metabolism of 10 chemicals in human and pig skin explants

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