Species-specific Expression of Major Urinary Proteins in the House Mice (Mus musculus musculus and Mus musculus domesticus)

Stopková, Romana; Stopka, Pavel; Janotová, Kateřina; Jedelský, Petr

doi:10.1007/s10886-007-9262-9

Cited by 52 publications

(63 citation statements)

References 35 publications

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“…Because MUPs represent substantial investment in excreted protein, we hypothesized that increased mating success and decreased survival in promiscuous-line males could be associated with up-regulated MUP expression. Urine collected from fourth-generation, individually housed adult males following the testosterone assay revealed that promiscuous-line males had higher MUP expression than monogamous-line males ( To identify specific up-regulated Mup loci in promiscuous-line males, we used reverse transcription quantitative qPCR (rt-qPCR) with nine primer sets from published studies that amplify most Mup transcripts (19,29) (Table S1). Because of extremely high sequence identity, some primer pairs were able to discriminate only subsets of paralogous Mup loci (29).…”

Section: Resultsmentioning

confidence: 99%

“…Several researchers have proposed that such expression is a result of sexual selection. This "honest-signal" model predicts that females favor males who can afford high expression despite potential costs (e.g., protein loss or attracting predators and competitors) (18)(19)(20)(21)(22). Although this hypothesis has not been explicitly tested, the observation that MUP concentration declines as mice adapt to captivity (17,23) suggests a possible role of relaxed social and sexual selection.…”

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confidence: 99%

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Reintroducing domesticated wild mice to sociality induces adaptive transgenerational effects on MUP expression

Nelson

Cauceglia

Merkley

et al. 2013

Proc. Natl. Acad. Sci. U.S.A.

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When brought into captivity, wild animals can adapt to domestication within 10 generations. Such adaptations may decrease fitness in natural conditions. Many selective pressures are disrupted in captivity, including social behavioral networks. Although lack of sociality in captivity appears to mediate domestication, the underlying mechanisms are not well understood. Additionally, determining the contribution of genetic inheritance vs. transgenerational effects during relaxed selection may provide insight into the flexibility of adaptation. When wild-derived mice kept under laboratory conditions for eight generations were reintroduced to sociality and promiscuity (free mate choice), they adapted within two generations. Fitness assessments between this promiscuous lineage and a monogamous laboratory lineage revealed malespecific effects. Promiscuous-line males had deficits in viability, but a striking advantage in attracting mates, and their scent marks were also more attractive to females. Here, we investigate mechanistic details underlying this olfactory signal and identify a role of major urinary protein (MUP) pheromones. Promiscuous-line males inherit higher MUP expression than monogamous-line males through transgenerational inheritance. Sociality-driven maternal and paternal effects reveal intriguing conflicts among parents and offspring over pheromone expression. MUP up-regulation is not driven by hormone-driven transduction pathways, but rather is associated with reduction in DNA methylation of a CpG dinucleotide in the promoter. This reduction in methylation could enhance transcription by promoting the binding of transcription factor USF1 (upstream stimulatory factor 1). Finally, we experimentally demonstrate that increased MUP expression is a female attractant. These results identify molecular mechanisms guiding domestication and adaptive responses to fluctuating sociality.social selection | sexy sons | epigenetics W ild animals bred in captivity have been observed to adapt to domestication within 10 generations (1-4). As a result, captive bred animals often have reduced fitness when reintroduced to natural conditions (5). Selective pressures disrupted by captivity include inbreeding avoidance, effective population size, disease exposure, predation, and sexual selection. For the latter example, evidence suggests that lack of social context for mate choice mediates adaptation to captivity (6). This context is especially relevant for social animals because the opportunity for mating success is regulated by hierarchical behavioral networks (i.e., social selection) (7), and this information is missing in captivity. The molecular mechanisms underlying phenotypes affected by lack of social selection are poorly understood. Furthermore, rapid adaptation to captivity raises questions about the roles of genetic inheritance vs. transgenerational effects (i.e., inheritance independent of genetic variation) (8). Resolving the mechanistic and hereditary basis of these transitions can provide insight into the flexibilit...

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Section: Resultsmentioning

confidence: 99%

mentioning

confidence: 99%

Reintroducing domesticated wild mice to sociality induces adaptive transgenerational effects on MUP expression

Nelson

Cauceglia

Merkley

et al. 2013

Proc. Natl. Acad. Sci. U.S.A.

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“…In one prominent example, distinctive profiles of MUPs are expressed in different strains, as well as in different subspecies, of mice (Robertson et al 1996;Stopková et al 2007). An atypical MUP that preferentially binds to 2-sec-butyl-4,5-dihydrothiazole is male-specific (Armstrong et al 2005).…”

Section: Mhc Odourtypes and Complexity Of Individual Odour Signaturesmentioning

confidence: 99%

In search of the chemical basis for MHC odourtypes

et al. 2010

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Mice can discriminate between chemosignals of individuals based solely on genetic differences confined to the major histocompatibility complex (MHC). Two different sets of compounds have been suggested: volatile compounds and non-volatile peptides. Here, we focus on volatiles and review a number of publications that have identified MHC-regulated compounds in inbred laboratory mice. Surprisingly, there is little agreement among different studies as to the identity of these compounds. One recent approach to specifying MHC-regulated compounds is to study volatile urinary profiles in mouse strains with varying MHC types, genetic backgrounds and different diets. An unexpected finding from these studies is that the concentrations of numerous compounds are influenced by interactions among these variables. As a result, only a few compounds can be identified that are consistently regulated by MHC variation alone. Nevertheless, since trained animals are readily able to discriminate the MHC differences, it is apparent that chemical studies are somehow missing important information underlying mouse recognition of MHC odourtypes. To make progress in this area, we propose a focus on the search for behaviourally relevant odourants rather than a random search for volatiles that are regulated by MHC variation. Furthermore, there is a need to consider a 'combinatorial odour recognition' code whereby patterns of volatile metabolites (the basis for odours) specify MHC odourtypes.

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“…This could be a starting point to determine how these proteins evolve through speciation (Hiadlovska et al, 2013), and their potential influence on sub-species recognition Ganem, 2002, 2008) and aggression (Dureje et al, 2011). These two sub-species have been previously found to vary quantitatively in the abundance of male VOCs (MucignatCaretta et al, 2010) and MUP expression between sexes (Stopková et al, 2007) with differences in the beta-barrel residues under selection (Karn and Laukaitis, 2012). Thus, we have identified new members of the odorant binding protein family and focused on the level and locations of expression of soluble lipocalins.…”

mentioning

confidence: 98%

“…Volatiles specifically bind to receptors of chemosensory neurons when released (Tirindelli et al, 1998;Novotny, 2003). In mice, the functions of lipocalin transporters are not well understood and most studies focused on the major urinary proteins (MUPs), which are expressed in the liver and transport volatile odor/organic compounds (VOCs) to the urine (Shahan and Derman, 1984;Shahan et al, 1987a,b;Stopková et al, 2007). MUPs have also been reported to be expressed in several tissues other than the liver (Shaw et al, 1983;Shahan et al, 1987a;Cavaggioni et al, 1999;Utsumi et al, 1999;Karn and Laukaitis, 2011), though their functions are not understood.…”

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confidence: 99%

Mouse Lipocalins (MUP, OBP, LCN) Are Co-expressed in Tissues Involved in Chemical Communication

et al. 2016

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Chemical communication is mediated by signal production and signal perception and in house mice (Mus musculus), both processes involve lipocalin proteins (OBP, MUP, LCN) that transport volatiles and protect them in tissues where they are produced. However, potential roles of lacrimal, nasal, and salivary lipocalins are still not well known. We aimed to determine the expression of the recently described family of odorant binding proteins (Obp), along with major urinary proteins (Mup) across different tissues in wild mice (Mus musculus) to assess the importance of these proteins based on their quantity in particular expression sites. We performed qPCR analysis of selected Mup, Lcn, Obp genes, and predicted Obp members to study their expression in selected tissues. We identified new members of the mouse odorant binding protein gene family in two subspecies, M. m. musculus and M. m. domesticus. We show that Mup4 and Mup5 from the phylogenetically older group-A are co-expressed with Obps in orofacial tissues. We also identified a sexually dimorphic pattern of female-biased Obp7 and male-biased Mup4 expression in lacrimal glands. OBPs, MUPs, and LCNs are produced in parallel, which may function to widen the spectrum of bound ligands, potentially including the degradation products of olfactory signals and/or toxic compounds. Moreover, our study demonstrates that several pheromone transporters from the lipocalin family are co-expressed in the nasal and lacrimal tissues of mice with the newly detected OBPs that further expand the already diverse mouse lipocalin family.

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Species-specific Expression of Major Urinary Proteins in the House Mice (Mus musculus musculus and Mus musculus domesticus)

Cited by 52 publications

References 35 publications

Reintroducing domesticated wild mice to sociality induces adaptive transgenerational effects on MUP expression

Reintroducing domesticated wild mice to sociality induces adaptive transgenerational effects on MUP expression

In search of the chemical basis for MHC odourtypes

Mouse Lipocalins (MUP, OBP, LCN) Are Co-expressed in Tissues Involved in Chemical Communication

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