Species-specific monoclonal antibodies in the assignment of the gene for human fibronectin to chromosome 2.

The locations of the genes for fibronectin (FN) on chromosomes of human germ line and somatic cells were determined by in situ molecular hybridization with two ³H-labeled DNA probes, one for the region encoding the cell attachment domain of human FN, the other for the 3’ noncoding and part of the coding region. Pachytene chromosomes of two males and lymphocyte chromosomes of one of these males and a female were used. Two regions of hybridization on pachytene and somatic chromosome 2 (p14→p16 and q34→q36) were found, but not in all individuals. A third region of hybridization was found at 11q12.1→ql3.5 in meiotic, but not with significant frequency in somatic chromosomes. It is not clear if these differences between meiotic and somatic chromosomes, and the large differences between individuals at some of the other hybridization sites, resulted solely from technical factors. The differences between the findings in meiotic and somatic preparations might be due to the presence of four strands in pachytene chromosomes versus only one per somatic chromatid. Individual differences in DNA sequences in the chromosome segment containing the gene, differences in gene locations among individuals, or between meiotic and mitotic chromosomes might account for the other findings. The results confirm some of the earlier studies with cell hybrids that mapped FN genes to chromosomes 2 or 11. The combined findings suggest that some of these loci may be coding for the plasma form of FN and others for the cellular form. The expression of the different FN types by differentiated cells might then depend on the loci that are activated.

show abstract

“…2 in hu man x mouse cell hybrids (Koch et al. 1982;Zardi et al, 1982;Prowse et al. 1985) and on the No.…”

Section: Discussionmentioning

confidence: 96%

“…8 carries a gene that specifies FN attachment to the cell membrane. Zardi et al (1982) also mapped FN to the No. 2 by determining the soluble FN secreted by human x mouse hybrids with an immunoelectrophorelic assay and species-specific antisera as well as with an ELISA assay and monoclonal antibodies.…”

mentioning

confidence: 99%

In situ localization of human fibronectin (FN) genes to chromosome regions 2p14→p16, 2q34→q36, and 11q12.1→q13.5 in germ line cells, but to chromosome 2 sites only in somatic cells

Jhanwar

Jensen

Kaelbling

et al. 1986

Cytogenet Genome Res

View full text Add to dashboard Cite

show abstract

“…These antibodies were derived from two in dependent hybrid clones as shown by the fact that they belong to two different Ig classes; however, competition binding experiments indicated that these antibodies are directed against the same or partially overlapping epi topes. Species-specific antibodies are useful reagents when it is important to unequivo cally establish the origin of fibronectin mole cules [33].…”

Section: Discussionmentioning

confidence: 99%

Monoclonal Antibodies to the Collagen Binding Domain of Human Plasma Fibronectin

Tarone¹,

Amedeo

Comoglio³

1984

Pathobiology

View full text Add to dashboard Cite

Five independent hybrids producing monoclonal antibodies to human plasma fibronectin have been obtained by fusing P3/X63-Ag8 myeloma cells with immune mouse splenocytes. The specificity of these monoclonal antibodies (MABs) for fibronectin was demonstrated by three independent tests: binding to the purified soluble molecule, immunofluorescence staining of insoluble extracellular matrices produced by endothelial cells in vitro, immunostaining of fibronectin tryptic peptides after separation on SDS-PAGE and transfer to nitrocellulose sheets. Two antibodies (MAB 29 and 52) recognized selectively human fibronectin while the others (MAB 5, 30 and 59) reacted also with plasma fibronectin from calf, hamster and chicken. Four distinct epitopes were recognized by the MABs studied. MAB 5, 30, 52 and 59 reacted with distinct antigenic sites, while MAB 29 and 52 bind to the same site. Antigenic fragments were identified by immunostaining of fibronectin tryptic peptides. MAB 5 reacted with a collagen binding fragment with a molecular weight of 120 K. In addition, each of the MAB 29, 30, 52 and 59 reacted with peptides with a molecular weight of 40 K that bind to gelatin. Since these antibodies do not inhibit fibronectin-collagen interaction, it is concluded that their corresponding epitopes are clustered in a region close, but not coincident, to the collagen binding site of fibronectin.

show abstract

“…16132 Genova, Italy the primary transcript of a single gene 16-131 localized on chromosome two [14,15].…”

Section: Fibronectinsmentioning

confidence: 99%

Localization of the cellular‐fibronectin‐specific epitope recognized by the monoclonal antibody IST‐9 using fusion proteins expressed in E. coli

et al. 1987

View full text Add to dashboard Cite

Here we report on a monoclonal antibody (IST-9) which distinguishes between human cellular and plasma fibronectin. Using /I-galactosidase-fibronectin fusion proteins expressed in E. coli we have demonstrated that this monoclonal antibody is specific for a fibronectin segment (ED) which can be included or omitted from the molecule depending on the pattern of splicing of the mRNA precursors. Furthermore, using the same fusion proteins we have been able to localize precisely the epitopes of two other monoclonal antibodies (IST-1 and IST-2), specific for the heparin-binding domain 5 of fibronectin.

show abstract

Species-specific monoclonal antibodies in the assignment of the gene for human fibronectin to chromosome 2.

Cited by 32 publications

References 29 publications

In situ localization of human fibronectin (FN) genes to chromosome regions 2p14→p16, 2q34→q36, and 11q12.1→q13.5 in germ line cells, but to chromosome 2 sites only in somatic cells

In situ localization of human fibronectin (FN) genes to chromosome regions 2p14→p16, 2q34→q36, and 11q12.1→q13.5 in germ line cells, but to chromosome 2 sites only in somatic cells

Monoclonal Antibodies to the Collagen Binding Domain of Human Plasma Fibronectin

Localization of the cellular‐fibronectin‐specific epitope recognized by the monoclonal antibody IST‐9 using fusion proteins expressed in E. coli

Contact Info

Product

Resources

About