In situ localization of human fibronectin (FN) genes to chromosome regions 2p14→p16, 2q34→q36, and 11q12.1→q13.5 in germ line cells, but to chromosome 2 sites only in somatic cells

Abstract

The locations of the genes for fibronectin (FN) on chromosomes of human germ line and somatic cells were determined by in situ molecular hybridization with two ³H-labeled DNA probes, one for the region encoding the cell attachment domain of human FN, the other for the 3’ noncoding and part of the coding region. Pachytene chromosomes of two males and lymphocyte chromosomes of one of these males and a female were used. Two regions of hybridization on pachytene and somatic chromosome 2 (p14→p16 and q34→q36) were found, but not in all individuals. A third region of hybridization was found at 11q12.1→ql3.5 in meiotic, but not with significant frequency in somatic chromosomes. It is not clear if these differences between meiotic and somatic chromosomes, and the large differences between individuals at some of the other hybridization sites, resulted solely from technical factors. The differences between the findings in meiotic and somatic preparations might be due to the presence of four strands in pachytene chromosomes versus only one per somatic chromatid. Individual differences in DNA sequences in the chromosome segment containing the gene, differences in gene locations among individuals, or between meiotic and mitotic chromosomes might account for the other findings. The results confirm some of the earlier studies with cell hybrids that mapped FN genes to chromosomes 2 or 11. The combined findings suggest that some of these loci may be coding for the plasma form of FN and others for the cellular form. The expression of the different FN types by differentiated cells might then depend on the loci that are activated.

An RFLP map for 2q33‐q37 from multicase mycobacterial and leishmanial disease families: no evidence for an Lsh/Ity/Bcg gene homologue influencing susceptibility to leprosy

“…Combining the mapping information from the different laboratories provides a consensus map for markers around Lsh on mouse chromosome 1 as follows : centromere-Cryg-0.7 cM-Crebl-3.3 cM--Fnl-3.1 cM-Tp-1-0-8 cM-Vil-1-0.3 cM-Des-0.3 cM-Inha-10.2 cM-Acrg-2.1 cM-ColGa3. This is broadly consistent with human in situ hybridization data mapping CRYG to 2q33-q36 (Shiloh et al 1986), CREBl to 2q32-q34 (Taylor et al 1990), FN1 to 2q34-q36 (Jhanwar et al 1986), TNPl ( = Tp-1) to 2q35-q36 (Luerssen et al 1990), VILl to 2q35q36 (Rousseau-Merck et al 1988), DES to 2q35 (Viegas-PBquignot et al 1989), INHA to 2q33-qter (Barton et al 1989), CHRNG ( = Acrg) to 2q32-qter (Cohen-Haguenauer et al 1987) and COL6A3 to 2q37 (Weil et al 1988). Lsh co-maps with (Schurr et al 1989;Malo et al 1991), or is very tightly linked to (Mock et al 1990), Vil-1 in the mouse, with Tp-1 and Des providing proximal and distal boundaries for the segment of chromosome 1 known to carry Lsh.…”

“…USA; Meakin et a l . 1985); MAP2 (microtubule associated protein 2, 2q33-q35; Pakstis et al 1990), the 2.4 kb EcoRI insert of KN7 (Neve et al 1986); FN1 (fibronectin 1, 2q34-q36; Jhanwar et al 1986), the 1-4 kb HindIII-BamHI fragment of pFH154 covering the central third of the full-length cDNA for the human fibronectin gene (ATCC 61038;Kornblitt et al 1983);VILl (villin, 2q35-q36 ;Rousseau-Merck et al 1988), the 550-bp BamHI-EcoRI fragment of pSP64 coding for the 110 carboxy terminal amino acids and 185 bp of the 3' untranslated region (Pringault et al 1986);DES (desmin, 2q35;Viegas-Pkquignot et al 1989), the 3 kb HindIII fragment of pHuDes3 coding for exons 1-6 of the human DES gene (Li et al 1989), COL6A3 (collagen type VI alpha 3, 2q37; Weil et a l . 1988), the 1.5 kb EcoRI insert of P24 (ATCC 61320); D2S55 (anonymous chromosome 2q marker; Lathrop et al 1987), a 3 kb EcoRI-Hind111 insert of pEKZ105 (ATCC 59352) ; or D2S3 (anonymous chromosome 2 marker, 2q35-q37 ; Litt et al 1986), the 2.4 kb EcoRI fragment of p5-2-96 (ATCC 59000).…”

Precise in situ localization of NCAM, ETS1, and D11S29 on human meiotic chromosomes

“…1986). This accessibility of specific decondensed regions of bivalents to enzymes could explain the additional regional hybridization of the libronectin gene found by Jhanwar et al (1986).…”

Fibronectin Interaction and Enhancement of Growth Factors: Importance for Wound Healing

“…20 A similar slippage mechanism involving the actin cytoskeleton and integrin receptors is used intracellularly. 19 Although FN comes from a single gene, 21 the resulting protein can exist in twenty different isoforms. 22 Depending on alternative splicing of a single 8 kb mRNA, yielding subunits range in size from 230 to 270 kDa.…”