1997
DOI: 10.1046/j.1365-294x.1997.d01-220.x
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Species‐specific primers discriminate schistosome intermediate hosts: unambiguous PCR diagnosis of Bulinus forskalii group taxa (Gastropoda: Planorbidae)

Abstract: The morphological definition of taxa has proved difficult within the Bulinus forskalii group, which includes intermediate hosts of medically important Schistosoma species in West Africa. Although B. forskalii and B. senegalensis transmit different schistosome species they are conchologically similar and their distributions overlap. Randomly amplified polymorphic DNA (RAPD) allows differentiation of sibling species in the genus Bulinus, but RAPDs are difficult to standardize, impairing their value as a taxonomi… Show more

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Cited by 4 publications
(7 citation statements)
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“…In the north of Cameroon B. forskalii overlaps in distribution (and may even occur in sympatry) with the closely related, morphologically similar Bulinus senegalensis (Goll 1981; Mimpfoundi & Greer 1989). Therefore, three species‐specific primers pairs which each amplify a single polymerase chain reaction (PCR) product were used in simple presence‐absence assays (primers 8 F and R, 8 F and 5 R, 5 F and R; Jones et al . 1997) to unambiguously identify B. forskalii individuals collected from the 14 northern Cameroon sites (sites 2–23, inclusive).…”
Section: Methodsmentioning
confidence: 99%
“…In the north of Cameroon B. forskalii overlaps in distribution (and may even occur in sympatry) with the closely related, morphologically similar Bulinus senegalensis (Goll 1981; Mimpfoundi & Greer 1989). Therefore, three species‐specific primers pairs which each amplify a single polymerase chain reaction (PCR) product were used in simple presence‐absence assays (primers 8 F and R, 8 F and 5 R, 5 F and R; Jones et al . 1997) to unambiguously identify B. forskalii individuals collected from the 14 northern Cameroon sites (sites 2–23, inclusive).…”
Section: Methodsmentioning
confidence: 99%
“…DNA was extracted from each individual hatchling using a proteinase K, phenol–chloroform procedure (Jones et al . 1997).…”
Section: Methodsmentioning
confidence: 99%
“…In addition to the PCR marker system for identi®cation of B. forskalii group snails as described by Jones, Noble, Lockyer et al (1997), 2 other genetic markers were chosen. Both the mitochondrial cyctochrome oxidase sub-unit I (COI) gene and the nuclear ribosomal internal transcribed spacer (ITS) region were targeted.…”
Section: Dna Analysismentioning
confidence: 99%
“…AF369610±12. Using the marker system of Jones, Noble, Lockyer et al (1997) for B. forskalii with primers 5F and 5R, an ampli®cation of c. 550 bp could be obtained from snails from sample sites 13 and 14. No similar sized ampli®cation products could be generated from snails from site 16, but fragments between 600±1000 bp were produced.…”
Section: Characterization Of Bulinus: Coimentioning
confidence: 99%
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