Species-specific responses of Antarctic terrestrial microalgae to salinity stress. Comparative study in Klebsormidium sp. and Stigeoclonium sp.

Canora, Diana Darriba; Llorens, Laura; Sicilia, Rebeca

doi:10.5817/cpr2022-1-7

Cited by 6 publications

(4 citation statements)

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“…A decreased FM was observed for all of the sensitive genotypes Konouz, Rihane, and ICARDA20 after irrigation with saline water, which was slightly higher in the semi-arid (KAI site) and arid (MED) environments. The decrease in FM was similar to observations made in different studies ( Canora et al., 2022 ). Salinity stress leads to a decrease in chlorophyll fluorescence signal, which flattens OJIP curves ( Figure 2 ).…”

Section: Discussionsupporting

confidence: 91%

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The efficiency of chlorophyll fluorescence as a selection criterion for salinity and climate aridity tolerance in barley genotypes

Hammami,

Tounsi-Hammami,

Nhamo

et al. 2024

Front. Plant Sci.

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IntroductionIn the Near East and North Africa (NENA) region, crop production is being affected by various abiotic factors, including freshwater scarcity, climate, and soil salinity. As a result, farmers in this region are in search of salt-tolerant crops that can thrive in these harsh environments, using poor-quality groundwater. The main staple food crop for most of the countries in this region, Tunisia included, is barley.MethodsThe present study was designed to investigate the sensitivity and tolerance of six distinct barley genotypes to aridity and salinity stresses in five different natural field environments by measuring their photosynthetic activity.Results and discussionThe results revealed that tolerant genotypes were significantly less affected by these stress factors than sensitive genotypes. The genotypes that were more susceptible to salinity and aridity stress exhibited a significant decline in their photosynthetic activity. Additionally, the fluorescence yields in growth phases J, I, and P declined significantly in the order of humid environment (BEJ), semi-arid site (KAI), and arid environment (MED) and became more significant when salt stress was added through the use of saline water for irrigation. The stress adversely affected the quantum yield of primary photochemistry (φP0), the quantum yield of electron transport (φE0), and the efficiency by trapped excitation (ψ0) in the vulnerable barley genotypes. Moreover, the performance index (PI) of the photosystem II (PSII) was found to be the most distinguishing parameter among the genotypes tested. The PI of sensitive genotypes was adversely affected by aridity and salinity. The PI of ICARDA20 and Konouz decreased by approximately 18% and 33%, respectively, when irrigated with non-saline water. The reduction was even greater, reaching 39%, for both genotypes when irrigated with saline water. However, tolerant genotypes Souihli and Batini 100/1B were less impacted by these stress factors.The fluorescence study provided insights into the photosynthetic apparatus of barley genotypes under stress. It enabled reliable salinity tolerance screening. Furthermore, the study confirmed that the chlorophyll a fluorescence induction curve had an inflection point (step K) even before the onset of visible signs of stress, indicating physiological disturbances, making chlorophyll fluorescence an effective tool for identifying salinity tolerance in barley.

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Section: Discussionsupporting

confidence: 91%

“…This phenomenon has been observed in different species exposed to salinity stress. It is attributed to a limitation in the quinones’ reoxidation rate, resulting in a reduced ability of PS II to transfer absorbed light energy to the photosynthetic linear electron transport chain ( Canora et al., 2022 ).…”

Section: Discussionmentioning

confidence: 99%

The efficiency of chlorophyll fluorescence as a selection criterion for salinity and climate aridity tolerance in barley genotypes

Hammami,

Tounsi-Hammami,

Nhamo

et al. 2024

Front. Plant Sci.

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“…The parameter Rfd is usually used for biotic and abiotic plant stress detection [82,83], and it is considered to be a good and rapid indicator of the vitality of plants. In many cases, it has already proved to be a very sensitive indicator of metabolic disturbances caused by different types of stress (e.g., salinity [84], nutrient enrichment [85], heavy metals [86]). It is reported to disturb the photochemical reactions in photosystem II, resulting in less efficient photosynthesis [85,86].…”

Section: Assessment Of the Degradation And Algal Toxicity Of A Potent...mentioning

confidence: 99%

Application of a Fluorescence-Based Instrument Prototype for Chlorophyll Measurements and Its Utility in an Herbicide Algal Ecotoxicity Assay

Lázár

Takács

Mörtl

et al. 2023

Water

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Project Aquafluosense was designed to develop prototypes for a modular fluorescence-based instrumental setup for in situ measurement of major water quality parameters. A fluorometer was developed for algal density estimation based on the fluorescent excitation of chlorophyll. The appropriate type of sample holder microplate was determined, along with the need for dark acclimation, prior to the measurements during the instrument’s development. Model species of green (Raphidocelis subcapitata) and blue-green alga (Microcystis aeruginosa) were applied in forms of pure monocultures and their mixtures, and improved analytical limits of detection were achieved (3.70 × 103 cell/mL and 1.13 × 105 for R. subcapitata and M. aeruginosa, respectively). The fluorescence-based determination of algal density was validated by conventional methods, such as cell counting in a Bürker chamber, optical density measurement, and chlorophyll extraction with ethanol. The signals obtained by the fluorometer correlated well with the conventional methods. Pearson r coefficients (applied where the correlation was linear) were ≥0.988 and Spearman ρ coefficients (applied where the correlation was not linear) were >0.976, indicating a strong and positive correlation. The applicability of the developed fluorometer was demonstrated in a growth inhibition ecotoxicity assay on R. subcapitata using the herbicide active ingredient isoxaflutole. During the assay, light intensity (continuous, 104.9 ± 14.9 µE/m2/s), temperature (22 ± 2 °C), pH of algal media (pH = 6–7 for Zehnder and Allen media, as well), and intensity of stirring (continuous, 100 rpm) were controlled. The results indicated that the FluoroMeter Module is applicable for screening algal toxicity: the observed ratio of fluorescence decrease determined by fluorescence induction provided significantly lower toxicity values (EC50: 0.015 ± 0.001 µg/mL) compared to values determined by the optical density (EC50: 0.034 ± 0.004 µg/mL) and chlorophyll a content (EC50: 0.033 ± 0.000 µg/mL).

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“…It plays an important role in plant development and adaptation to abiotic stressors such as salt, drought, and cold [31]. Salt stress significantly increases the content of major carbohydrates, which can be involved in osmotic regulation and used as a protective agent for cell homeostasis regulation during salt stress tolerance [32][33][34][35].…”

Section: Expression Of Resistance-related Genes Was Upregulated Under...mentioning

confidence: 99%

Transcriptomic Analysis of Ulva prolifera in Response to Salt Stress

Zhuo

Wang

et al. 2022

Water

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Since 2007, Ulva prolifera, a green macroalgal species that blooms periodically in the Yellow Sea of China, has caused the world’s biggest green tide, which induced harmful ecological impacts and economic losses. When the alga is subject to prolonged exposure to air, it suffers abiotic stresses. To explore the physiological and molecular mechanisms of salt stress, the transcriptome data of U. prolifera at different salinities (30, 50, and 90 psu) were obtained by high-throughput sequencing using the Illumina HiSeq platform, and photosynthetic physiological parameters were also measured. The results showed that a total of 89,626 unigenes were obtained after de novo assembly, of which 60,441 unigenes were annotated in the databases (NR, NT, KO, SWISS-PROT, PFAM, GO, and KOG). GO functional enrichment analysis revealed that the enrichment of differentially expressed genes (DEGs) was mainly in cellular, cell, and binding processes. KEGG metabolic pathway enrichment analysis showed that the most frequently enriched pathways of DEGs included glycolysis, pyruvate metabolism, peroxisome, and fatty acid biosynthesis. In addition, resistance-associated proteins, such as heat-shock proteins, microtubule-associated proteins, ubiquitin-associated proteins, abscisic-acid-signaling-pathway-associated proteins, and antioxidant-related proteins are upregulated under salt stress. Genes associated with photosynthesis and carbon fixation pathways are also upregulated, accompanied by an increase in photosynthetic oxygen release rates. These findings provide a basis for understanding the molecular mechanisms of the response of U. prolifera to salinity change, thus providing a theoretical basis for the analysis of the green tide outbreak mechanism.

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Species-specific responses of Antarctic terrestrial microalgae to salinity stress. Comparative study in Klebsormidium sp. and Stigeoclonium sp.

Cited by 6 publications

References 46 publications

The efficiency of chlorophyll fluorescence as a selection criterion for salinity and climate aridity tolerance in barley genotypes

The efficiency of chlorophyll fluorescence as a selection criterion for salinity and climate aridity tolerance in barley genotypes

Application of a Fluorescence-Based Instrument Prototype for Chlorophyll Measurements and Its Utility in an Herbicide Algal Ecotoxicity Assay

Transcriptomic Analysis of Ulva prolifera in Response to Salt Stress

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