Species-specific virulence of Vibrio harveyi for black tiger shrimp is associated with bacteriophage-mediated hemocyte agglutination

Intaraprasong, Aungkul; Khemayan, Krit; Pasharawipas, Tirasak; Flegel, Timothy W.

doi:10.1016/j.aquaculture.2009.08.005

Cited by 14 publications

(13 citation statements)

References 39 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Esto concuerda con los estudios realizados en Penaeus monodon, en los que se encontraron graves daños similares ante la infección de Vibrio harveyi, siendo los principales efectos la formación de nódulos y la agregación de hemocitos con presencia inusual en arterias y senos hemales (Intaraprasong et al, 2009).…”

Section: Resultados Y Discusiónunclassified

Análisis histopatológico en Litopenaeus vannamei infectado con Vibrio parahaemolyticus.

Navarro

Mejías

2015

Agron. Mesoam.

View full text Add to dashboard Cite

Section: Resultados Y Discusiónunclassified

Análisis histopatológico en Litopenaeus vannamei infectado con Vibrio parahaemolyticus.

Navarro

Mejías

2015

Agron. Mesoam.

View full text Add to dashboard Cite

“…3). Not surprisingly, V. harveyi ECPs induced degeneration of Penaeus monodon intestinal matrix cells, characterized by prominent pyknosis (Intaraprasong et al 2009). …”

Section: Discussionmentioning

confidence: 99%

Injection of Acanthaster planci with thiosulfate-citrate-bile-sucrose agar (TCBS). II. Histopathological changes

Rivera-Posada¹,

Pratchett²,

Owens³

2011

Dis. Aquat. Org.

View full text Add to dashboard Cite

ABSTRACT:We assessed histological changes in the tissues of the crown-of-thorns starfish Acanthaster planci (COTS) after injection of thiosulfate-citrate-bile-sucrose agar (TCBS) which was used as a disease inducer (potential outbreak control method), by conventional and scanning electron microscopy. Digestive glands were processed and stained with hematoxylin and eosin to describe the histological architecture of the intestinal epithelium. Subsequently comparison of healthy versus infected tissues and Gram stains were carried out to confirm bacterial occurrence on infected tissues, characterize the structural changes induced by bacterial communities in COTS tissues, and to determine if the histopathological changes of intestinal tissues were consistent with vibrio infection. TCBS injections induced marked epithelial desquamation, hypertrophy and hypersecretion of glandular cells, epithelial cell destruction, pyknosis, reduction of thickness and disorganization of connective tissue and associated nerve plexus, presence of bacterial colonies, irregular eosinophilic foci in glandular cells, brush border disruption, atrophy and detachment of intestinal microvilli and cell debris in the lumen. All these changes were attributed to a fulminating systemic dysbiosis and were consistent with vibrio infections.

show abstract

“…In addition, culture supernatant solutions from VH1 are highly toxic for the black tiger shrimp (Penaeus monodon) but not for the whiteleg shrimp (Penaeus [Litopenaeus] vannamei) (13). Comparison of mass spectrometry data from protein bands originating from semipurified fractions of these toxic supernatant solutions showed no significant homology to protein data (direct and deduced) in existing databases (13), particularly for Vibrio species. Since no significant homology was found in the Vibrio database, it was suggested that the toxins might have originated from the VHS1 genome.…”

mentioning

confidence: 99%

“…Since the phage is carried in the lysogens as an episome, cured isolates can be obtained at high rates by isolation of single colonies upon subculture (14). Random clones representing approximately 25% of the VHS1 genome were previously sequenced and deposited in GenBank (26).Vibrio harveyi 1114GL (VH0) that has been lysogenized by VHS1 (VH1) is Ͼ100 times more lethal to the black tiger shrimp than VH0 (13,14). In addition, culture supernatant solutions from VH1 are highly toxic for the black tiger shrimp (Penaeus monodon) but not for the whiteleg shrimp (Penaeus [Litopenaeus] vannamei) (13).…”

mentioning

confidence: 99%

See 1 more Smart Citation

Complete Genome Sequence of Virulence-Enhancing Siphophage VHS1 from Vibrio harveyi

Khemayan

Prachumwat

Sonthayanon

et al. 2012

Appl Environ Microbiol

Self Cite

View full text Add to dashboard Cite

ABSTRACTVibrio harveyisiphophage 1 (VHS1) is a tailed phage with an icosahedral head of approximately 66 nm in diameter and an unornamented, flexible tail of approximately 153 nm in length. WhenVibrio harveyi1114GL is lysogenized with VHS1, its virulence for the black tiger shrimp (Penaeus monodon) increases by more than 100 times, and this coincides with production of a toxin(s) associated with shrimp hemocyte agglutination. Curiously, the lysogen does not show increased virulence for the whiteleg shrimp (Penaeus[Litopenaeus]vannamei). Here we present and annotate the complete, circular genome of VHS1 (81,509 kbp; GenBank accession numberJF713456). By software analysis, the genome contains 125 putative open reading frames (ORFs), all of which appear to be located on the same DNA strand, similar to the case for many other bacteriophages. Most of the putative ORFs show no significant homology to known sequences in GenBank. Notable exceptions are ORFs for a putative DNA polymerase and putative phage structural proteins, including a portal protein, a phage tail tape measure protein, and a phage head protein. The last protein was identified as a component of the species-specific toxin mixture described above as being associated with agglutination of hemocytes fromP. monodon.

show abstract

Species-specific virulence of Vibrio harveyi for black tiger shrimp is associated with bacteriophage-mediated hemocyte agglutination

Cited by 14 publications

References 39 publications

Análisis histopatológico en Litopenaeus vannamei infectado con Vibrio parahaemolyticus.

Análisis histopatológico en Litopenaeus vannamei infectado con Vibrio parahaemolyticus.

Injection of Acanthaster planci with thiosulfate-citrate-bile-sucrose agar (TCBS). II. Histopathological changes

Complete Genome Sequence of Virulence-Enhancing Siphophage VHS1 from Vibrio harveyi

Contact Info

Product

Resources

About