Specific 3H-imipramine binding in human platelets

Langer, Salomón Z.; Briley, Mike; Raisman, Rita; Henry, J. F.; Morselli, P. L.

doi:10.1007/bf00505732

Cited by 185 publications

(31 citation statements)

References 28 publications

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“…To confirm our findings with these two drugs, a separate group of studies was performed using citalopram, a 5-HT selective reuptake inhibitor (SSRI) which is the most selective and potent inhibitor of the 5-HT reuptake site (K, = 2.6 nM) presently available. It has a higher potency ratio of DA to 5-HT and norepinephrine to 5-HT, than other SSRI's (i.e., paroxetine, fluoxetine, sertraline, quipazine, 6-nitroquipazine; Hytell et al, 1977Hytell et al, , 1982Langer et al, 1980;Koe et al, 1983;Thomas et al, 1987;Hashimoto and Goromaru, 1990;Johnson, 1993). The SSRI's, particularly citalopram, have very weak affinities for neurotransmitter receptors, especially when compared with the tricyclic antidepressants and should produce the opposite effect of a 5-HT antagonist as it causes an increase in synaptic concentrations of 5-HT.…”

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confidence: 99%

Serotonergic modulation of striatal dopamine measured with positron emission tomography (PET) and in vivo microdialysis

et al. 1995

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Positron emission tomography and in vivo microdialysis were used to study serotonin's role in modulating striatal dopamine. Serial PET studies were performed in adult female baboons at baseline and following drug treatment, using the dopamine (D2) selective radiotracer, 11C-raclopride. The serotonergic system was manipulated by administration of the selective 5-HT reuptake inhibitor, citalopram, or by serotonergic (5-HT2) receptor blockade (using altanserin, a 5-HT2 antagonist). 11C-Raclopride time-activity data from striatum and cerebellum were combined with plasma arterial input functions and analyzed by calculating a distribution volume as described previously (Logan et al., 1990). Additionally, in vivo microdialysis studies were performed in awake freely moving rats using similar pharmacologic challenges plus SR 46349B, a new highly selective 5-HT2 receptor antagonist. Altanserin and SR 46349B increased extracellular striatal dopamine concentrations (35% and 910%, respectively) while altanserin decreased striatal 11C-raclopride binding (37%). Citalopram, however, decreased extracellular striatal dopamine concentrations (50%) and increased 11C-raclopride binding (33%). These data demonstrate that 5- HT-selective drugs produce changes in striatal dopamine that can be measured noninvasively with PET. Furthermore, the PET data obtained from anesthetized baboons is consistent with in vivo microdialysis data obtained from awake and freely moving rats. Finally, these studies have implications for understanding the therapeutic efficacy of atypical neuroleptics and their utility for treating schizophrenia and affective disorders.

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confidence: 99%

Serotonergic modulation of striatal dopamine measured with positron emission tomography (PET) and in vivo microdialysis

et al. 1995

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“…In another study of 35 subjects aged 19-97, B max but not K d for [ 3 H]imipramine binding decreased significantly with increasing age [7]. In contrast, no significant correlations between age and platelet [ 3 H]paroxetine binding were found in a study of 45 healthy subjects aged 15-95 [8] paroxetine has a twofold higher proportion of specific binding to the serotonin uptake site [11], indicating that it is a more appropriate radioligand than [ 3 H]imipramine for studies of the platelet serotonin transporter.…”

Section: Introductionmentioning

confidence: 99%

Binding of [3H]lysergic Acid Diethylamide to Serotonin 5-HT2A Receptors and of [3H]paroxetine to Serotonin Uptake Sites in Platelets from Healthy Children, Adolescents and Adults

Sigurdh

Spigset

Allard³

et al. 1999

Neuropsychobiology

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Possible age effects on binding of [³H]lysergic acid diethylamide ([³H]LSD) to serotonin 5-HT_2A receptors and of [³H]paroxetine to serotonin uptake sites were studied in platelets from healthy children (11–12 years of age), adolescents (16–17 years of age) and adults. Significant overall age effects were found both for the number of binding sites (B_max) for [³H]LSD binding (p < 0.001), the affinity constant (K_d) for [³H]LSD binding (p < 0.001), B_max for [³H]paroxetine binding (p < 0.001) and K_d for [³H] paroxetine binding (p = 0.006). In general, there was a decrease in B_max with increasing age, which predominantly occurred between the ages 11–12 years and 16–17 years for the 5-HT_2A receptor, and after 16–17 years of age for the serotonin uptake site. These developmental changes might have an impact on the effect of treatment with serotonergic drugs in children and adolescents. When the platelet serotonin variables investigated are employed in studies in children or adolescents, age matching or, alternatively, introduction of age control in the statistical analysis should be performed.

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“…One potential source of this variability is the heterogeneity in demographic characteristics of research subjects. Specifically, with the possible exception of two studies [Asarch el al., 1980;Langer et al, 1980], age and sex effects on imipramine binding sites have not been studied systematically. More over, the extent to which the data of Langer et al [1980] are informative is unclear since their control population included both normal subjects and nonpsychiatric hospi tal patients with unspecified medical disorders; the possi bility of sex and age interactions with platelet binding sites in these disorders was not explored.…”

Section: Introductionmentioning

confidence: 99%

High Affinity 3H-Imipramine Binding in Human Platelets: Age and Sex Effects

Baron¹,

Kowalik²,

Barkai³

et al. 1984

Neuropsychobiology

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Age and sex effects on ³H-imipramine platelet binding sites were determined in 58 normal subjects (27 males, 31 females). The correlation of age with either the maximal imipramine binding (B_max) or the dissociation constant (K_d) was statistically nonsignificant in both males and females. Males did not differ from females in B_max and K_d values. The implications for psychiatric research were discussed.

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Specific 3H-imipramine binding in human platelets

Cited by 185 publications

References 28 publications

Serotonergic modulation of striatal dopamine measured with positron emission tomography (PET) and in vivo microdialysis

Serotonergic modulation of striatal dopamine measured with positron emission tomography (PET) and in vivo microdialysis

Binding of [<sup>3</sup>H]lysergic Acid Diethylamide to Serotonin 5-HT<sub>2A</sub> Receptors and of [<sup>3</sup>H]paroxetine to Serotonin Uptake Sites in Platelets from Healthy Children, Adolescents and Adults

High Affinity <sup>3</sup>H-Imipramine Binding in Human Platelets: Age and Sex Effects

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