Specific adhesion of carcinoembryonic antigen-bearing colorectal cancer cells to immobilized carcinoembryonic antigen

Levin, Larissa V.; Griffin, Thomas W.

doi:10.1016/0304-3835(91)90221-3

Cited by 18 publications

(7 citation statements)

References 17 publications

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“…The functional assay employed in this work was homotypic adhesion between stable transfectants of CHO-derived LR-73 cells in suspension. Evidence involving binding of purified CEA molecules to CEA transfectant cells and colorectal carcinoma cells (43,44) or immobilized purified CEA (Fuks and Stanners, unpublished) 4 strongly favors the view that this adhesion is mediated by homophilic bonds between antiparallel CEA molecules on apposite cell surfaces. The model building analysis employed here utilized the known structure of CD2 as a guide and indicated that region 1, G 30 YSWYK, and the adjacent region 2, N 42 RQII, are surface exposed.…”

Section: Discussionmentioning

confidence: 99%

Self recognition in the Ig superfamily: Identification of precise sub domains in CEA required for intercellular adhesion

Taheri

Saragovi

Fuks

et al. 2000

Journal of Biological Chemistry

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Section: Discussionmentioning

confidence: 99%

Self recognition in the Ig superfamily: Identification of precise sub domains in CEA required for intercellular adhesion

Taheri

Saragovi

Fuks

et al. 2000

Journal of Biological Chemistry

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“…In fact, the specific binding of neisserial Opa protein to CEACAM was reported to be mediated through the N-domain (23,24). The involvement of the N-domain in homo-and heterophilic adhesions between CEA family members has been also demonstrated by using chimeric and deleted constructs (16,18,25) or by using mAbs recognizing the N-domain (17,26,27). In the case of CEACAM1 (BGP), Teixeira et al proposed N-domain-Ndomain interaction (25).…”

Section: Only the N-terminal Two Thirds Of The N-domain Of Ceacam8 Ismentioning

confidence: 96%

Extracellular N-Domain Alone Can Mediate Specific Heterophilic Adhesion between Members of the Carcinoembryonic Antigen Family, CEACAM6 and CEACAM8

Oikawa

Sugiyama

Kuroki

et al. 2000

Biochemical and Biophysical Research Communications

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“…Certain colorectal carcinoma cell lines that express CEA on their surface will bind to CEA-coated plastic (liostcttcr et ul.. 1990u,h;Levin and Griffin, 1991;Levin et d., 1992). Because CHO cells transfcctcd with functional CEA also adhere to CEA-coated plastic, a homophilic interaction may take place between the CEA expressed on the cells and the CEA on the plastic.…”

Section: Discussionmentioning

confidence: 99%

“…More recently Zhou et al (1990) found that CEA-and NCA-producing transfectants can form he terotypic aggregates but will not aggregate with other immunoglobulin supergene family members, such as NCAM, that are not in the immediate CEA gene family. Hostetter et al (1990 a,b) and Levin and Griffin (1991) also demonstrated that human colorectal carcinoma cells bind to CEA attached to a solid surface. These experiments suggest that CEA and NCA are homophilic binding proteins that mediate homotypic cell aggregation.…”

mentioning

confidence: 96%

Adhesion to carcinoembryonic antigen by human colorectal carcinoma cells involves at least two epitopes

Jessup

Kim

Thomas

et al. 1993

Intl Journal of Cancer

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Carcinoembryonic antigen (CEA) may be involved in both cell-cell and cell-substrate adhesion. Our purpose was to determine whether epitopes involved in the homophilic binding of human colorectal carcinoma cells to CEA participated in adhesion to basement membrane proteins. Three human colorectal adenocarcinoma cell lines and one CHO cell line transfected with CEA cDNA were tested in a solid-phase adhesion assay. The 2 CEA-expressing carcinoma cell lines (KM-12c and CCL 188) and the transfectant, but not the parental CHO line, bound to CEA. The CEA-non-producing carcinoma line (Clone A) did not bind to CEA. All colorectal carcinoma cell lines, the transfectant and the parental CHO line bound to laminin, while the colorectal carcinoma lines bound to type-IV collagen. MAbs to epitopes on CEA that cross-react with non-specific cross-reacting antigen (NCA) inhibited adhesion of CEA-expressing cells to CEA. MAbs to non-cross-reactive epitopes of CEA did not block adhesion to CEA. When the inhibitory anti-CEA antibodies were compared in a competitive radioimmunoassay, 2 distinct epitopes were identified. Epitope I is in the N-terminal domain and defined by MAbs MN3, T84.1 and C110, whereas epitope II is located in the repeating loop domains and is recognized by antibodies MN15, PR3B10 and NP1. None of the antibodies to epitope I or II blocked adhesion by KM-12c or CCL 188 cells to laminin or type-IV collagen. Thus, at least 2 different regions on CEA participate in adhesion to CEA but not to collagen or laminin by CEA-expressing human colorectal carcinoma cells.

show abstract

Specific adhesion of carcinoembryonic antigen-bearing colorectal cancer cells to immobilized carcinoembryonic antigen

Cited by 18 publications

References 17 publications

Self recognition in the Ig superfamily: Identification of precise sub domains in CEA required for intercellular adhesion

Self recognition in the Ig superfamily: Identification of precise sub domains in CEA required for intercellular adhesion

Extracellular N-Domain Alone Can Mediate Specific Heterophilic Adhesion between Members of the Carcinoembryonic Antigen Family, CEACAM6 and CEACAM8

Adhesion to carcinoembryonic antigen by human colorectal carcinoma cells involves at least two epitopes

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