Specific and Cross-Reacting Antibodies in Human Responses to Onchocerca volvulus and Dracunculus medinensis Infections

Gárate, Teresa; Kliks, Michael M.; Cabrera, Z.; Parkhouse, R. M. E.

doi:10.4269/ajtmh.1990.42.140

Cited by 24 publications

(12 citation statements)

References 0 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The specificity of Trichinella CLE antigens can be improved by mild alkali O deglycosylation, but antibodies to antigens thus treated still retain unacceptable cross-reactivity, mainly with sera from patients infected with filariae and Dracunculus, which are closely related antigenically (5,16). These data suggest that only some of the cross-reacting epitopes contained in CLE glycoproteins are O-glycans in nature.…”

Section: Discussionmentioning

confidence: 79%

Evaluation ofTrichinella spiralisLarva Group 1 Antigens for Serodiagnosis of Human Trichinellosis

et al. 2004

View full text Add to dashboard Cite

To identify Trichinella antigens suitable for high-specificity and high-sensitivity serodiagnosis of human trichinellosis, we evaluated assays using four antigens: (i) crude first-stage larval extract (CLE), (ii) Odeglycosylated CLE, (iii) tyvelose-bearing antigens (Trichinella spiralis larva group 1 [TSL-1] antigens) purified by US4 affinity chromatography and coupled directly to enzyme-linked immunosorbent assay (ELISA) plates (pTSL-1 antigens), and (iv) TSL-1 antigens immobilized on ELISA plates with the monoclonal antibody (MAb) US4 (cTSL-1 antigens). Assays using these antigens were compared by analysis of sera from healthy individuals (n ‫؍‬ 224) (group 1), individuals with noninfectious intestinal pathologies (n ‫؍‬ 114) (group 2), individuals with other parasitic infections (n ‫؍‬ 107) (group 3), and individuals with confirmed trichinellosis (n ‫؍‬ 42) (group 4). Our results indicate that capture ELISA using cTSL-1 antigens is the most effective method for serodiagnosis of human trichinellosis; this was the only method showing 100% specificity and 100% sensitivity at the patent stage of the infection, and it was also the most sensitive for sera obtained prior to patency in indirect immunofluorescence (IIF). Indirect ELISA with pTSL-1 antigens was also 100% specific but was slightly less sensitive, particularly with sera obtained before IIF patency. Inhibition ELISA with MAb US4 indicated (i) that in Trichinella-infected patients the immune response to TSL-1 antigens is directed mostly against tyvelose-containing epitopes (mean of 84.2% of total anti-TSL-1 immunoglobulin G1 [IgG1] antibody response [range, 51.3 to 97.6%]) and (ii) that in most individuals a large proportion of anti-CLE IgG1 antibodies (mean, 49.5%; range, 7.3 to 92.6%) are directed against tyvelose epitopes.

show abstract

Section: Discussionmentioning

confidence: 79%

Evaluation ofTrichinella spiralisLarva Group 1 Antigens for Serodiagnosis of Human Trichinellosis

et al. 2004

View full text Add to dashboard Cite

show abstract

“…It is important to note that highly restricted cross-reactions between Onchocerca volvulus and other nematodes have been reported for IgE and IgG4 antibodies. 35,36 Based on the above information, we propose that a crude FWAP/IgG4 ELISA may be used as an economical, rapid, sensitive, and specific test for serologic diagnosis of human fascioliasis. However, as for human schistosomiasis, 31 FWAP-specific IgG4 levels did not decrease significantly following chemotherapy.…”

Section: Discussionmentioning

confidence: 99%

Parasite-specific antibody profile in human fascioliasis: application for immunodiagnosis of infection.

Maher¹,

Ridi²,

Elhoda³

et al. 1999

The American Journal of Tropical Medicine and Hygiene

View full text Add to dashboard Cite

Abstract. The antibody isotype response to an adult Fasciola worm antigen preparation (FWAP) was examined in sera from 60 Egyptians with parasitologically confirmed fascioliasis by an ELISA. The FWAP-specific IgG1 and IgG4 antibodies were found in 97-100% of the patients. The ratio of the mean absorbance values between infected patients and healthy controls was 9.7 and 29.7 for IgG1 and IgG4 antibodies, respectively. The IgM, IgA, IgG2, and IgG3 antibodies were less dominant. In contrast to IgG1 antibodies, which were often detected in sera from patients infected with Schistosoma, Echinococcus granulosus, Ascaris lumbricoides, Ancylostoma duodenale, or Hymenolepis nana, FWAP-specific IgG4 antibodies were detected exclusively in the sera of patients with fascioliasis. The data thus support the conclusion that an IgG4/ELISA with crude FWAP as antigen may be used for sensitive and accurate immunodiagnosis of human fascioliasis.

show abstract

“…Besides the use of antigenic fractions (such as surface or excretion-secretion components) or the study of antibody at isotype level, the absorption of sera with heterologous antigens or conserved molecules such as phosphorylcholine (Pery et al 1974) may represent an inexpensive alternative strategy to reduce cross-reactivity. Pre-absorption or competitive assays have been reported in tests for filariasis, toxocariasis and hookworm infections (Almond & Parkhouse 1986;Lynch et al 1988;Garate et al 1990;Chieffi et al 1990).…”

Section: Standardization Of the Elisamentioning

confidence: 99%

Seroepidemiology of abdominal angiostrongyliasis: the standardization of an immunoenzymatic assay and prevalence of antibodies in two localities in Southern Brazil

Graeff-Teixeira¹,

Agostini²,

Camillo-Coura³

et al. 1997

Tropical Med Int Health

View full text Add to dashboard Cite

SummaryAbdominal angiostrongyliasis is a nematode disease produced by Angiostrongylus costaricensis, a metastrongylid parasite of wild rodents. Accidental human infection occurs through ingestion of food or water contaminated with third-stage larvae present in the mucous secretion of terrestrial molluscs. An ELISA test was standardized for detection of IgG antibodies recognizing a surface antigen prepared from female worms. Competitive absorption of sera with Ascaris suum crude antigen resulted in a test with 86% sensitivity and 83% specificity. The disease is endemic in Southern Brazil and a number of cases are diagnosed every year through anatomo-pathological examination of biopsies or surgical specimens, since no other diagnostic method is available. According to seroepidemiological studies, prevalences in two transmission foci are 29.8 and 66%, attesting to the widespread occurrence of the infection in those endemic areas. keywords angiostrongyliasis, ELISA, Brazil correspondence C. Graeff-Teixeira, Rua Marquês do Pombal, 799/801, CEP 90540-010,

show abstract

Specific and Cross-Reacting Antibodies in Human Responses to Onchocerca volvulus and Dracunculus medinensis Infections

Cited by 24 publications

References 0 publications

Evaluation ofTrichinella spiralisLarva Group 1 Antigens for Serodiagnosis of Human Trichinellosis

Evaluation ofTrichinella spiralisLarva Group 1 Antigens for Serodiagnosis of Human Trichinellosis

Parasite-specific antibody profile in human fascioliasis: application for immunodiagnosis of infection.

Seroepidemiology of abdominal angiostrongyliasis: the standardization of an immunoenzymatic assay and prevalence of antibodies in two localities in Southern Brazil

Contact Info

Product

Resources

About