Specific and practicable assessment of urinary free cortisol by combination of automatic high-pressure liquid chromatography and radioimmunoassay

Estimation of Urinary Unconjugated Androstenedione, Dehydroepiandrosterone, Testosterone, Cortisol, Aldosterone and 18-Hydroxycorticosterone as a Potential Tool for Assessing Adrenal Status

“…As far äs we have been able to ascertain, the reference ranges for urinary steroids found with the present method are comparable or slightly lower than those previously described in the literature (8,10,11,13,14,(16)(17)(18). Corresponding data on the excretion of urinary unconjugated dehydroepiandrosterone have not been documented so far.…”

“…Reagents such äs organic solvente labelled and unlabelled steroids, buffer Solutions, radioimmunoassay (RIA) components and the antisera against cortisol, aldosterone and 18-hydroxycorticosteronc have been listed in prcviously published papers (12,13). The antisera against androstencdione, tcstosterone and dehydroepiandrosterone were raised in rabbits by immunization with antigens coupled at C-3 (androstendione and dehydroepiandrosterone) or C-ll (testosterone) to bovine serum ailbumin.…”

Rapid and specific determination of free cortisol in guinea pig urine and faeces by thin-layer chromatography-competitive protein-binding assay

“…The CPBA offers some advantages over previous methods, including the use of dexamethasone as a marker for cortisol localization on TLC sheets, the use of buffer instead of organic solvents to remove cortisol from silica gel and the speed of the radioassay (1-2 h). The specificity of the TLC-CPBA method is comparable with corresponding HPLC-RIA techniques [18,19]. The practicability of the TLC-CPBA seems better than that of a HPLC-RIA since cortisol is easily isolated and quantitatively separated from the silica gel in the TLC-CPBA method, and since the separation of cortisol by TLC is more rapid than by HPLC [20]: 10 samples are applied to plates within 70 minutes, and plates are developed within 30 rain.…”

Cortisol Assays: Guidelines for the Provision of a Clinical Biochemistry Service

“…[31][32][33][34][35][36][37][38][39][40] Competitive protein binding methods give clinically useful results, but specific radioimmunoassay avoids some problems with their lack of speciticity. " Interference by derivatives of cortisol and progesterone may occasionally occur in adrenal disease and the polycystic ovary syndrome; this can manifest itself as inappropriately elevated UFC in the presence of low normal serum cortisol.…”