Specific and sensitive quantitation of medroxyprogesterone acetate in human serum by gas chromatography—mass spectrometry

Quantitative determination of medroxyprogesterone acetate in plasma by liquid chromatography/electrospray ion trap mass spectrometry

“…This is the lowest concentration of the analyte that can be measured with a coefficient of variation and accuracy both less than 20%. The present assay using HPLC/electrospray ion trap mass spectrometry was found to be 10 times more sensitive compared with HPLC or gas chromatography/mass spectrometry methods previously published 10–13…”

“…Different methods for the extraction of MPA in plasma are described in the literature using C18 solid phase extraction 11–13. We tried to extract MPA in plasma with various organic solvents.…”

“…Therefore, plasma levels of MPA are relatively low and also show large inter‐individual differences. The reported methods include radioimmunoassay, gas chromatography with electron capture detection, high‐performance liquid chromatography (HPLC), and gas chromatography/mass spectrometry 9–13. However, none of these methods can be applied in pharmacokinetic studies with low doses of MPA due to low detection sensitivity.…”

A stability-indicating HPLC method for medroxyprogesterone acetate in bulk drug and injection formulation

“…Up to now, several methods including spectrophotometry [4,5], HPLC [6][7][8][9], LC-MS [10][11][12][13][14][15][16], GC-MS [16][17][18][19][20] and immunoassay [21] have been published for determining MPA in pharmaceutical preparations and biological fluids. Two stabilityindicating HPLC methods for MPA tablet formulations have been reported.…”

Simultaneous Determination of Estradiol Valerate and Medroxyprogesterone Acetate in a Tablet Formulation by Gas Chromatography-Mass Spectrometry

“…2,3 The combination estrogen-gestagen is used for the treatment of estrogenic deficiency syndrome to control its symptoms (climateric syndrome), such as loss of bone minerals and development of heart disease. 4 Up to now, various analytical methods for the determination of EV or MPA have been reported including voltammetry, 1 spectrophotometry, 5,6 fluorometry, 7 high performance liquid chromatography (HPLC), 4,[8][9][10][11] gas chromatography-mass spectrometry (GC-MS), [12][13][14] FT-Raman spectroscopy 15 and chemiluminescence enzyme immunoassay 16 in pharmaceutical preparations and biological liquids. But there has been no GC-MS method reported in the litearature for simultaneous determination of these two hormones in pharmaceutical preparations.…”