Specific bacteriophage of Bordetella bronchiseptica regulates B. bronchiseptica-induced microRNA expression profiles to decrease inflammation in swine nasal turbinate cells

Abstract

Background Respiratory diseases in pigs are the main health concerns for swine producers. Similar to the diseases in human and other animals, respiratory diseases are primary related to morbidity and are the result of infection with bacteria, viruses, or both. B. bronchiseptica causes serious respiratory diseases in the swine airway track. However, the B. bronchisepticaspecific bacteriophage has diverse advantages such as decreasing antibiotic overuse and possible therapeutic potential against bacteria.Objective The objects of this study were to investigate the therapeutic effect of specific B. bronchiseptica bacteriophages and to identify genes related to bacteriophage signaling utilizing RNA microarrays in swine nasal turbinate cells. Methods Bor-BRP-1 phages were applied 24 h prior to B.bronchiseptica infection (1 × 10 7 cfu/ml) at several concentrations of bacterial infection. Cells were incubated to detect cytokines and 24 h to detect mucin production. And real-time quantitative PCR was performed to examine related genes expression. To determine the change of total gene expression based on B.bronchiseptica and Bor-BRP-1 treatment, we performed RNA sequencing experiments. Results The results showed that B. bronchiseptica induced increased expression of several inflammatory genes such as IL-1β, IL-6, and Muc1 in a dose-dependent manner. However, Bor-BRP-1 induced reduction of gene expression compared to the B. bronchiseptica induction group. In addition, microarrays detected Bor-BRP-1-altered inflammatory gene expression against B. bronchiseptica, reducing B. bronchiseptica-induced airway inflammation in swine epithelial cells. Conclusion These results suggest that the specific bacteriophage has a therapeutic potential to defend against B. bronchiseptica infection by altering inflammatory gene expression profiles. Song declare that we have no conflict of interest.Ethical approval This article does not contain any studies with human subjects or animals performed by any of the authors.

The forecasting power of the mucin-microbiome interplay in livestock respiratory diseases

“…Bordetella bronchiseptica increases MUC1 production in swine nasal turbinate cells (Park et al. 2020 ). In line with this, viruses can also stimulate MUC5AC overproduction in vitro or in vivo on airway epithelial cells (Thai et al.…”

Phage Therapy in Veterinary Medicine

“…The bacteriophage against B. bronchiseptica is capable of inhibiting the production of cytokines and mucin, the overproduction, which is responsible for exaggerated inflammatory responses. This characteristic of the bacteriophage to regulate the inflammatory response has positive implications for the treatment of respiratory diseases in pigs [ 88 , 89 ].…”