Specific Capsular Polysaccharide of Type 45
            <i>Streptococcus pneumoniae</i>
            (American Type 72)

Daoust, Virginia; Carlo, Dennis J.; Zeltner, Johanna Y.; Perry, Malcolm B.

doi:10.1128/iai.32.3.1028-1033.1981

Cited by 14 publications

(9 citation statements)

References 9 publications

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“…Capsular polysaccharide was dissolved in 5 % ammonium hydroxide and left overnight at 37 "C. The excess ammonia was evaporated under a stream of nitrogen and the sample desalted on a column of Bio-Gel P2. Type 20 polysaccharide was obtained and purified as previously described (Daoust et al, 1981;Richards and Perry, 1982).…”

Section: Isolation and Purification Of Capsular Antigen The Cellsmentioning

confidence: 99%

Identification of the Common Antigenic Determinant Shared by Streptococcus pneumoniae Serotypes 35A and 20 Capsular Polysaccharides — Structural Analysis of the Streptococcus pneumoniae Serotype 35A Capsular Polysaccharide

Beynon

Richards

Perry

1997

European Journal of Biochemistry

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The specific polysaccharide antigen of Streptococcus pneumoniae serotype 35A was shown, by a combination of one-and two-dimensional NMR methods and chemical analyses, to be a high-molecularmass polymer composed of D-galactose, D-glucose, mannitol, and phosphate (3 : 1 : 1 : 1). The pentasaccharide repeating unit is polymerized through phosphate diester linkages to give the structure, Ac AcAc0-Acetyl substituents are present at postions 5 and 6 of the 3)-P-~-Galfresidue and at position 2 of the 6)-P-~-Galf residue. The capsular polysaccharides of S. pneumoniae serotypes 20 and 35A both contain the disaccharide unit -3)-p-~-Galf-( l-?i)-p-~-Gkp-( 1-which is the probable structural determinant responsible for the serological cross reactivity of the two polysaccharides.Keywords: Streptococcus pneumoniae; polysaccharide ; structure ; antigenic determinant.Streptococcus pneumoniae is the most common etiological agent of bacterial pneumonia which until recently was easily treated with antibiotics. However, in the last 25 years the increase in antibiotic resistance of S. pneumoniae has presented major problems for patients and clinicians: there are as many as 40 000 deaths per year in the USA alone resulting from pneumococcal infections (Fedson et al., 1994). Since the capsular polysaccharides (CPS) of S. pneumoniae are responsible for stimulation of the host's immune system, vaccines have been developed from the CPS of the major pneumococcal serotypes responsible for infections in humans. Information on the structure (charge, hydrophobicity and conformational expression of epitopes) of the polysaccharide antigen is essential in the development of a new generation of vaccines and in explaining the serological cross-reactivity exhibited by S. pneumonine serogroups.Within the group 35 of S. pneumoniae four serotypes (35F, 35A, 35B, and 35C) have been recognized and, by the use of factor sera, seven factors (20b, 29b, 34b, 35a, 35b, 35c, and 42a) have been determined (Lund and Henrichsen, 1978 MATERIALS AND METHODSOrganisms and growth conditions. S. pneumoniae serotype 35A bacterial cells (Eddy 62, Statens Seruminstitut: NRCC 4757) were grown overnight on blood agar plates (Quelab) and then suspended in Bacto Todd-Hewitt broth (Difco, 15 g/l) and Bacto Columbia broth (Difco, 17.5 gll) in a 20-1 carboy, with the pH adjusted to between 7.50-7.80 with NaOH. The carboy was incubated in 5 % carbon dioxide, overnight, at 37°C. The cells were killed with 4% formalin (final concentration), left overnight at 4"C, prior to harvesting by centrifugation.Isolation and purification of capsular antigen. The cells were washed with 1 % phenol, the wash solution was dialysed, concentrated, and the CPS was recovered by precipitation with ethanol ( 5 vol.). It was further purified by precipitation with 1 % cetyltrimethylammonium bromide and, after keeping overnight at 4"C, the polysaccharide was recovered by centrifugation, redissolved in 10% sodium chloride, and precipitated with 5 vol. ethanol. The polysaccharide was dissolved in water, di...

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Section: Isolation and Purification Of Capsular Antigen The Cellsmentioning

confidence: 99%

Identification of the Common Antigenic Determinant Shared by Streptococcus pneumoniae Serotypes 35A and 20 Capsular Polysaccharides — Structural Analysis of the Streptococcus pneumoniae Serotype 35A Capsular Polysaccharide

Beynon

Richards

Perry

1997

European Journal of Biochemistry

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“…Proposed structure for the amino sugar purified from the teichuronic acid (Crumpton & Davies, 1958) and Pseudomonas aeruginosa (Knirel et al, 1982) and the capsular polysaccharides of Staphylococcus aureus (Liau et al, 1974). The L-form of the sugar is present in the antigenic capsular polysaccharides of Bacteroides fragilis (Kasper et al, 1983), Streptococcus pneumoniae (Daoust et al, 1981) and Pneumococcus type V (Barker et al, 1961). These antigenic capsular polysaccharides are well known to be strain-specific structures and to differ in different strains belonging to even the same species.…”

Section: Results and Discussion Purification Of The Amino Sugarmentioning

confidence: 99%

Presence of fucosamine in teichuronic acid of the alkalophilic Bacillus strain C-125

Aono¹,

Uramoto²

1986

Biochemical Journal

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Cell walls of the alkalophilic Bacillus strain C-125 are composed of gamma-peptidoglycan, teichuronic acid and a polymer of glucuronate and glutamate. An amino sugar that was a main component of the teichuronic acid did not correspond to any of the commercially available hexosamines. The amino sugar was purified into crystalline form from the hydrolysate of the teichuronic acid by ion-exchange chromatography and then partition chromatography on a cellulose column. The amino sugar was identified as D-fucosamine (2-amino-2,6-dideoxy-D-galactose) by 400 MHz n.m.r. spectrometric analysis, measurement of optical rotation and elemental analysis.

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“…The crude polysaccharide (220 mg) was fractionated by diethylaminoethyl (DEAE)-Sepharose CL-6B chromatography in tris(hydroxymethyl)aminomethane-hydrochloride buffer (0.1 M, pH 7.6) as previously described (7) to yield two fractions. One fraction (31%) eluting in the buffer showed no reactivity with the type 46 pneumococcal antiserum.…”

Section: Resultsmentioning

confidence: 99%

“…Experimental methods were the same as those described for the analysis of the type 45 S. pneumoniae polysaccharide (7).…”

Section: Methodsmentioning

confidence: 99%

Specific Capsular Polysaccharide of Type 46 Streptococcus pneumoniae (American Type 73)

1981

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The specific capsular polysaccharide of type 46 Streptococcus pneumoniae (American type 73) was isolated in pure form and shown to be a high-molecular-weight, glycosidically linked polymer composed of d -galactose (2 mol), N -acetyl- d -glucosamine (1 mol), N -acetyl- d -galactosamine (1 mol), and N -acetyl- l -fucosamine (1 mol).

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Specific Capsular Polysaccharide of Type 45 Streptococcus pneumoniae (American Type 72)

Cited by 14 publications

References 9 publications

Identification of the Common Antigenic Determinant Shared by Streptococcus pneumoniae Serotypes 35A and 20 Capsular Polysaccharides — Structural Analysis of the Streptococcus pneumoniae Serotype 35A Capsular Polysaccharide

Identification of the Common Antigenic Determinant Shared by Streptococcus pneumoniae Serotypes 35A and 20 Capsular Polysaccharides — Structural Analysis of the Streptococcus pneumoniae Serotype 35A Capsular Polysaccharide

Presence of fucosamine in teichuronic acid of the alkalophilic Bacillus strain C-125

Specific Capsular Polysaccharide of Type 46 Streptococcus pneumoniae (American Type 73)

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