Specific detection of fungal pathogens by 18S rRNA gene PCR in microbial keratitis

Zunaina, Embong; Hitam, Wan Hazabbah Wan; Yean, Chan Yean; Rashid, Nur Haslindawaty Abdul; Kamarudin, Balqis; Abidin, Siti Khaironi Zainal; Osman, Sabariah; Zafarina, Zainuddin; Ravichandran, Manickam

doi:10.1186/1471-2415-8-7

Cited by 69 publications

(52 citation statements)

References 20 publications

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“…In nonendemic areas, systemic mycosis is seen in individuals who visit endemic areas, with the risk of acquisition increasing with immune suppression. 6,7 The asexual form A. dermatitidis exists as a mold in nature. Airborne conidia, when inhaled, gets lodged in the alveoli, where it undergoes transition to yeast form, which is seen in mammalian tissue and culture at 37 C. Wet earth enriched with animal droppings, rotting wood, and decaying vegetables is considered to be the ecological niche for B. dermatitidis.…”

Section: Discussionmentioning

confidence: 99%

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Autochthonous Blastomycosis of the Adrenal: First Case Report from Asia

Kumar

Sreehari

et al. 2014

The American Society of Tropical Medicine and Hygiene

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Abstract. Systemic endemic mycoses, such as blastomycosis, are rare in Asia and have been reported as health risks among travelers who visit or reside in an endemic area. Adrenal involvement is rarely seen in blastomycosis and has never been reported from Asia. We report the first case of blastomycosis with bilateral involvement of the adrenals in a diabetic patient residing in the state of Arunachal Pradesh, India.

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Section: Discussionmentioning

confidence: 99%

“…6 DNA extraction was done using the yeast Genomic DNA Kit (Himedia Laboratories Pvt. Ltd., Mumbai, India).…”

Section: Case Reportmentioning

confidence: 99%

Autochthonous Blastomycosis of the Adrenal: First Case Report from Asia

Kumar

Sreehari

et al. 2014

The American Society of Tropical Medicine and Hygiene

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“…PCR primers used to detect the 18S rRNA gene of Aspergillus sp. : PFPRIM-F3 5 0 GACTCAACACGGGGAA ACT3 0 and PFPRIM-R4 5 0 ATTCCTCGTTGAAGAGCA3 0 (Embong et al 2008). The amplification reaction was conducted in 50 ll that contained 19 Taq DNA polymerase buffer (10 mM Tris-HCl, pH 9.0, 50 mM NaCl, 0.01 M Triton X-100, and 2 mM MgCl 2 ), 2 mM of each dNTP, 10 mM of each primer, 50 ng of genomic DNA, and 5 units of Taq DNA polymerase.…”

Section: Isolation and Screening Of Decolorizing Fungal Isolatesmentioning

confidence: 99%

Isolation, identification, and biochemical characterization of a brown rot fungus capable of textile dye decolorization

Gomaa

Momtaz

Kareem

et al. 2010

World J Microbiol Biotechnol

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Twenty-two local brown rot fungal isolates were obtained from 5 different environmental sources. Fourteen isolates were presumptively identified as Aspergillus sp. and eight as Penicillium sp. using mycelium and spore morphology. All the fungal isolates were screened for their ability to decolorize Isolan Red and colored waste water and to produce oxidase activity. Aspergillus isolate 2 was chosen for further study because it could decolorize both dyes and produce oxidase. A 400 bp fragment of the 18S rRNA gene from isolate 2 was analyzed by nucleotide sequence analysis. Blastn analysis of sequence data demonstrated 100% identity to Aspergillus sp. and isolate 2 was assigned the strain designation Aspergillus sp. EL-2 (Accession number: HM140797). EL-2 could remove up to 80% of Disperse Blue in waste water effluent within 48 h in submerged shake culture. The decolorization process was energy dependent, growth related, and required viable biomass. EL-2 was able to grow and decolorize waste water over a broad pH range. Addition of inducers and inhibitors of specific enzymes or families of enzymes demonstrated the involvement of phenol oxidase (laccase), cytochrome p-450 oxygenase and hydroxyl radicals in the decolorization process. The data also suggest that it may be practical to enhance decolorizing activity of Aspergillus sp. EL-2 through the metabolic control of fungal degradative pathways by altering media composition.

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“…Otros autores han encontrado que Fusarium spp. es el principal patógeno encontrado en las muestras oculares (24,25,26).…”

Section: Carlos Pérez Díaz Patricia Barrios Castañeda José David MIunclassified

Colección corneal intraestromal y queratitis por Fusarium Spp: presentación de dos casos y revisión de la literatura

Díaz

Castañeda

Echavarría³

et al. 2013

Rev. Med.

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ResumenLas infecciones oculares por hongos filamentosos han aumentado su incidencia con el pasar de los años. Los traumas corneales, la terapia inmunosupresora y el uso de lentes de contacto han hecho que éstas aumenten su incidencia, pero existe un pequeño porcentaje de paciente que sin ningún factor de riesgo presentan infecciones por estos mohos. En este artículo reportamos dos casos de infección ocular por Fusarium spp. en dos pacientes de 21 y 30 años de edad respectivamente, inmunocompetentes, pertenecientes a las Fuerzas Militares y sin factores de riesgo asociados.Palabras Clave: Queratitis fúngica, moho, Fusarium spp. COLLECTION INTRASTROMAL CORNEAL AND KERATITIS BY FUSARIUM SPP: REPORT OF TWO CASES AND REVIEW OF THE LITERATURE AbstractThe filamentous fungal eye infections have been increasing in incidence in the last years. Corneal trauma, immunosuppressive therapy and use of contact lenses have increased their incidence, nonetheless a small percentage of patients with no risk factors still present with these mold infections. In this article we report a case of ocular infection by Fusarium spp. in two patients, a 21-year-old and a 30-year-old males. They work with the Military Forces, and were immunocompetent with no risk factors associated to the problem.

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Specific detection of fungal pathogens by 18S rRNA gene PCR in microbial keratitis

Abstract: Background: The sensitivity and specificity of 18S rRNA polymerase chain reaction (PCR) in the detection of fungal aetiology of microbial keratitis was determined in thirty patients with clinical diagnosis of microbial keratitis.

Cited by 69 publications

References 20 publications

Autochthonous Blastomycosis of the Adrenal: First Case Report from Asia

Autochthonous Blastomycosis of the Adrenal: First Case Report from Asia

Isolation, identification, and biochemical characterization of a brown rot fungus capable of textile dye decolorization

Colección corneal intraestromal y queratitis por Fusarium Spp: presentación de dos casos y revisión de la literatura

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