SPECIFIC DETECTION OF IgM-ANTIBODIES BY ELISA, APPLIED IN HEPATITIS-A

Duermeyer, W.; Veen, J. Van Der

doi:10.1016/s0140-6736(78)92802-7

Cited by 107 publications

(30 citation statements)

References 4 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…19 More recently, the capture principle has been applied for the detection of other immunoglobulin isotypes such as IgE 20,21 and IgA. 22,23 The binding of a representative part of the total IgE and subsequent detection of anti-P. brasiliensis IgE reflects the relative amount of specific versus total IgE, and not the absolute concentration of specific IgE as measured by indirect ELISA.…”

Section: Resultsmentioning

confidence: 99%

Capture enzyme-linked immunosorbent assay to detect specific immunoglobulin E in sera of patients with paracoccidioidomycosis.

Mamoni¹,

Rossi²,

Camargo³

et al. 2001

The American Journal of Tropical Medicine and Hygiene

View full text Add to dashboard Cite

Abstract. Paracoccidioidomycosis (PCM) is the most frequent systemic mycosis in South America. The disease is characterized by a polyclonal activation of B cells, resulting in hyperimmunoglobulinemia. The production of immunoglobulin (Ig) E in deep mycosis has been related to the severity of the disease. However, the detection of specific IgE in sera of patients is difficult because of the competition with the IgG. We compared a capture and an indirect enzyme-linked immunosorbent assay (ELISA) technique to detect Paracoccidioides brasiliensis IgE. We found that the capture ELISA presented higher performance and lower background values than the indirect assay, resulting in a significant quantitative discrimination between sera from patients with the 2 major clinical forms of PCM. Patients with the juvenile form presented significantly higher levels of P. brasiliensis IgE, as compared with patients with the adult form. The capture ELISA was used in the follow-up of patients receiving treatment, showing that the levels of specific IgE decreased as the patient's clinical conditions improved.

show abstract

Section: Resultsmentioning

confidence: 99%

Capture enzyme-linked immunosorbent assay to detect specific immunoglobulin E in sera of patients with paracoccidioidomycosis.

Mamoni¹,

Rossi²,

Camargo³

et al. 2001

The American Journal of Tropical Medicine and Hygiene

View full text Add to dashboard Cite

show abstract

“…Another problem is that for several reasons a second serum sample is often unavailable, and in these cases the detection of specific IgM may be the only serological way to provide a diagnosis of recent infection. Several groups of workers, including ourselves, have preliminary findings on such a test using either ELISA or RIA and the results look promising (Frosner et al, personal communication 1978;Duermeyer and van der Veen, 1978).…”

Section: Discussionmentioning

confidence: 99%

A radioimmunoassay for hepatitis A antigen and antibody.

Skidmore¹,

Boxall²

1979

J Clin Pathol

View full text Add to dashboard Cite

show abstract

“…The principle on whch the test described in this paper is based is already used widely in diagnosing acute viral infections such as hepatitis A [2] ,hepatitis B [4], cytomegalovirus [14], and rubella virus [8] , and this test method is as sensitive for demonstrating anti-TBEV IgM. In sera drawn in the acute phase of illness and up to 7 weeks after onset of illness, anti-TBEV IgM could be demonstrated in dilutions up to lop4.…”

Section: Scussl Onmentioning

confidence: 99%

Serological diagnosis of acute tick‐borne encephalitis by demonstration of antibodies of the IgM class

Roggendorf

Heinz

Deinhardt

et al. 1981

Journal of Medical Virology

View full text Add to dashboard Cite

A sensitive enzyme immunoassay is described for demonstrating specific antibodies of the IgM class to tick-borne encephalitis virus (anti-TBEV IgM). Anti-mu-coated, flat-bottomed microtiter plates are incubated with diluted patients' serum (2 hr at 37 degrees C), then with purified TBEV, and later with peroxidase-coupled anti-TBEV immunoglobulin for a further 2 hr. After washing the plates, orthophenylenediamine is added and the optical density is measured at 510 nm. At an early stage after onset of illness anti-TBEV IgM could be demonstrated up to serum dilutions of 10(-4). The specificity of the test system was shown by total inhibition of the positive reaction by preincubation of patients' sera with anti-mu. Tick-borne encephalitis (TBE) could be diagnosed serologically in 54 patients by the described method, and anti-TBEV IgM could be demonstrated in sera drawn up to 9 months after onset of illness. A peak in the number of infections occurs in the age group of 31-40 years, and most infections occur from June to October with a small peak in July.

show abstract

SPECIFIC DETECTION OF IgM-ANTIBODIES BY ELISA, APPLIED IN HEPATITIS-A

Cited by 107 publications

References 4 publications

Capture enzyme-linked immunosorbent assay to detect specific immunoglobulin E in sera of patients with paracoccidioidomycosis.

Capture enzyme-linked immunosorbent assay to detect specific immunoglobulin E in sera of patients with paracoccidioidomycosis.

A radioimmunoassay for hepatitis A antigen and antibody.

Serological diagnosis of acute tick‐borne encephalitis by demonstration of antibodies of the IgM class

Contact Info

Product

Resources

About