Specific Detection of Tomato Pathotype of Verticillium dahliae by PCR Assays

Usami, Toshiyuki; Abiko, Mafumi; Shishido, Masahiro; Amemiya, Yoshimiki

doi:10.1007/pl00013066

Cited by 25 publications

(18 citation statements)

References 20 publications

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“…Polymerase chain reaction and sequencing Genomic DNA of each fungal strain was extracted using a standard phenol-chloroform extraction method (Usami et al 2002 or a QIAamp Tissue Kit (Qiagen GmbH, Hilden, Germany). The genomic region of ribosomal DNA ITS1-5.8S-ITS2 was amplified using PCR primers ITS1 and ITS4 (White et al 1990).…”

Section: Pathogenicity Testmentioning

confidence: 99%

Verticillium tricorpus causing lettuce wilt in Japan differs genetically from California lettuce isolates

et al. 2010

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In January 2002, Verticillium wilt of lettuce (Lactuca sativa L.) caused by Verticillium tricorpus occurred in upland paddy fields in Hyogo Prefecture for the first time in Japan. This fungal species was first isolated from lettuce in California, USA. In the present study, the genetic relationships between the American and Japanese isolates of V. tricorpus from lettuce were analyzed to determine whether the pathogen could have migrated to Japan from the USA, the major lettuce-seed supplier for Japan. Nucleotide sequences of the rDNA internal transcribed spacer regions, as well as the genes coding for translation elongation factor 1-alpha and RNA polymerase II were compared among American and Japanese V. tricorpus isolates from lettuce. The Japanese isolates of V. tricorpus were distinct from the American. Random amplified polymorphic DNA analyses also supported this conclusion. These results demonstrated that Verticillium wilt on lettuce caused by V. tricorpus in Japan was not related to the isolates causing the disease in California.

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Section: Pathogenicity Testmentioning

confidence: 99%

Verticillium tricorpus causing lettuce wilt in Japan differs genetically from California lettuce isolates

et al. 2010

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“…1). For the experiment, total DNA of each isolate was prepared as previously described (Usami et al 2002(Usami et al , 2005. We prepared a PCR reaction mixture (20 ll) containing 20 ng fungal total DNA, 100 pmol each of primers, VdMAT1-2a (5 0 -CGA CCGCTACTATATTGGCCC-3 0 ; nt 3327-3347 of accession AB505214) and VdMAT1-2b (5 0 -CTGCGACAGCA GATTCTGGGTTGCAAAGGC-3 0 ; nt 3932-3903 of accession AB505214) (Usami et al 2009), and 10 ll of Premix Ex Taq Hot Start Version (Takara Bio, Otsu, Shiga, Japan).…”

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confidence: 99%

Asexual fungus Verticillium dahliae is potentially heterothallic

2009

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Verticillium dahliae, a soilborne plant pathogen, causes wilt disease in many important crops. We reported previously that the mating type gene MAT1-2-1 is spread to isolates of this asexual fungus. However, we did not determine whether V. dahliae is homothallic or heterothallic because the opposite mating type gene, MAT1-1-1, had not been identified. In the present study, we identified the MAT1-1-1 gene from an isolate lacking MAT1-2-1 and the mating type idiomorphs of V. dahliae. Each isolate we tested contained either the MAT1-1 or MAT1-2 idiomorph, indicating that the asexual fungus V. dahliae is potentially heterothallic.

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“…dahliae is a pathogen that lacks host specificity, although some isolates are regarded as host-specific (22) or classified as distinct pathotypes (24,37,54). Usually, isolates of V. dahliae are considered to be host-adapted rather than host-specific, since isolates have the potential to infect a wide range of hosts but often seem to be most virulent toward certain hosts (11).…”

Section: Discussionmentioning

confidence: 99%

Phylogenetic Analyses of Phytopathogenic Isolates of Verticillium spp.

Qin¹,

Vallad²,

Wu³

et al. 2006

Phytopathology®

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To better understand the genetic relationships between Verticillium dahliae isolates from lettuce and other phytopathogenic Verticillium spp. isolates from various hosts and geographic locations, the complete intergenic spacer (IGS) region of the nuclear ribosomal RNA gene (rDNA) and the β-tubulin gene were amplified and sequenced. The sequences of the complete IGS region and the β-tubulin gene were used alone and in combination to infer genetic relationships among different isolates of Verticillium with the maximum-likelihood distance method. Phylogenetic analyses set sequences into four distinct groups comprising isolates of V. albo-atrum, V. tricorpus, and V. dahliae from cruciferous and noncruciferous hosts. Within the four Verticillium groups, isolates of V. dahliae from cruciferous hosts displayed the closest affinity to V. dahliae from noncruciferous hosts. Isolates of V. dahliae from noncruciferous hosts could be further divided into four subgroups based on sequence similarities within the IGS region. Cross-pathogenicity tests demonstrated that most Verticillium isolates were as virulent on other hosts as on their hosts of origin. A phenogram based on the cross pathogenicity of individual isolates resembled those derived from the IGS and β-tubulin sequence comparisons. On the basis of the data presented, the potential origin of some isolates of V. dahliae pathogenic on lettuce is proposed.

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Specific Detection of Tomato Pathotype of Verticillium dahliae by PCR Assays

Cited by 25 publications

References 20 publications

Verticillium tricorpus causing lettuce wilt in Japan differs genetically from California lettuce isolates

Verticillium tricorpus causing lettuce wilt in Japan differs genetically from California lettuce isolates

Asexual fungus Verticillium dahliae is potentially heterothallic

Phylogenetic Analyses of Phytopathogenic Isolates of Verticillium spp.

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