Specific determination of cysteine in human urine by capillary micellar electrokinetic chromatography

Ševčíková, Petra; Glatz, Zdeněk

doi:10.1002/jssc.200301372

Cited by 17 publications

(10 citation statements)

References 31 publications

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“…Although the method was primarily developed for the determination of glutathione in red blood cells , its applicability was further extended to the determination of homocysteine in human plasma (Fig. ), cysteine in human urine and to monitor DTT clearance in biological and chemical samples with LODs in the micromolar range.…”

Section: Application Of On‐column Derivatisationmentioning

confidence: 99%

On‐capillary derivatisation as an approach to enhancing sensitivity in capillary electrophoresis

Glatz

2014

Electrophoresis

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Separation technologies play an important role in revealing biological processes at various omic levels, in pharmacological and clinical research. In this context, CE is a strong candidate for analyses of samples with rapidly increasing complexity. Even though CE is well known for its many advantages in this regard, the sensitivity of CE analyses is insufficient for many applications. Accordingly, there are generally three main options for enhancing the sensitivity of CE analyses - using special detection techniques, using sample pre-concentration and derivatisation. Derivatisation is often the method of choice for many laboratories, since it is simple and provides several advantages such as small sample volume demand and the possibility of automation. Although it can be performed in different ways depending on where the reaction takes place, this article reviews one of the simplest and at the same time most useful approaches on-capillary derivatisation. Even if in many cases the use of on-capillary derivatisation alone is enough to improve the detection sensitivity, on other occasions it needs to be employed in combination with the other above-mentioned strategies. After a simple discussion of derivatisation in general, special attention is focused on the on-capillary approach and methodologies available for on-capillary reactant mixing. Its applications in various fields are also described.

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Section: Application Of On‐column Derivatisationmentioning

confidence: 99%

On‐capillary derivatisation as an approach to enhancing sensitivity in capillary electrophoresis

Glatz

2014

Electrophoresis

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“…At very high concentration of the cysteine (3000 ppm), the modafinil recovery lowered to 41.8%, but the concentration of cysteine in urine is lower than 121 ppm. [24] The results of the selectivity study show that none of the selected molecules have interference, and the assays are selective against competitive molecules.…”

Section: Methods Validationmentioning

confidence: 98%

SERS‐based assays for sensitive detection of modafinil

Çağlayan

Torul

Onur

et al. 2015

J Raman Spectroscopy

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In this paper, we proposed two sensitive surface‐enhanced Raman spectroscopy assays for the determination of modafinil in urine matrix. In the first assay, modafinil was extracted by solid phase extraction and determined after sandwiching with silver nanoparticles. In the second assay, modafinil was extracted by non‐specific interaction with magnetic gold nanoparticles and determined by sandwiching with magnetic gold and silver nanoparticles. The non‐specific magnetic extraction does not require any analyte specific agent like antibody, aptamer, or molecularly imprinted polymer, therefore, the cost and complexity of the assay is very low. Both assays are capable for application to urine samples with the detection limits under the minimum required performance limit of modafinil. The assays were validated in terms of accuracy, precision, detection limits, and ranges. Copyright © 2015 John Wiley & Sons, Ltd.

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“…Its applicability has been further expanded by this group on the monitoring of DTT in biological and chemical systems [39], on the detection of cysteine in human urine [40], and on the determination of homocysteine in human plasma [41]. The principle of this incapillary detection system is very straightforward.…”

Section: Applications Of In-capillary Derivatization and Chemical Reamentioning

confidence: 99%

Advances in capillary electrophoretically mediated microanalysis: An update

2006

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This review, as a continuation of an earlier report, gives an overview of recent developments, over the period from 2003 until now, in the use of capillary electrophoretic techniques for the in-line study of enzymatic reactions, derivatization, and chemical reactions. The article is divided into two parts: (i) in-line enzymatic reactions and (ii) in-line derivatization and chemical reactions. The first part introduces electrophoretically mediated microanalysis (EMMA) and discusses and illustrates the different modes of EMMA. A literature overview on enzymatic reactions is provided. The second part starts with an introduction of the procedures and the nomenclature used in the area of in-line derivatization and chemical reactions based on EMMA. Reported derivatization and chemical reaction procedures are discussed and summarized.

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Specific determination of cysteine in human urine by capillary micellar electrokinetic chromatography

Cited by 17 publications

References 31 publications

On‐capillary derivatisation as an approach to enhancing sensitivity in capillary electrophoresis

On‐capillary derivatisation as an approach to enhancing sensitivity in capillary electrophoresis

SERS‐based assays for sensitive detection of modafinil

Advances in capillary electrophoretically mediated microanalysis: An update

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