Specific extraction of human serum albumin by partition in aqueous biphasic systems containing poly(ethylene glycol) bound ligand

The adsorption of globular proteins onto a fluorinated PDMS surface

“…In two-phase (liquid) organic-water mixtures, the protein surface hydrophobicity has been reported to make a significant contribution to the partitioning behavior of the protein between the organic and aqueous phases [26][27][28][29][30][31]. In our situation, the protein is partitioned between a solution phase of varying aqueous character and the solid-phase substrate which is either hydrophilic, hydrophobic or fluorophilic.…”

Hydrophobic Affinity Cell Partition Detects Alterations in Surface Properties of Erythrocytes in Rats Bearing a Subcutaneous Leydig Cell Tumour

“…Cell partition, expressed as the per centage of cells added to the system that were present in the top phase, was performed in triplicate, in the absence and presence of P-PEG. P-PEG was synthe sised by the method of Shanbhag and Johansson [ 16] from palmitoyl chloride and PEG 6000, and reprecipi tated five times from absolute ethanol. 37% of the hydroxyl groups were esterified and no free palmitic acid was detectable.…”

Membrane surface properties reflected by cell partition in two‐polymer aqueous phases Classes of beef erythrocytes having different membrane lipid, charge and affinity for a ligand

“…They were prepared as previously described [ I] and had the following compositions: System 1 -5% (w/w) dextran TSOO, lot No. 3936 (Pharmacia Fine Chemicals, Piscataway, NJ), 4% (w/w) polyethylene glycol6000 (PEG, tradename 'Carbowax', Union Carbide, New York) and 0.11 M Na-phosphate buffer, pH 6.8; System 2 -4.5% (w/w) dextran, 3.6% (w/w) PEG, 0.15 M NaCl and 0.01 M Na-phosphate buffer, pH 6.8; System 3 -5% (w/w) dextran, 3.5% (w/w) PEG, 0.15 M NaCl, 0.01 M Na-phosphate buffer, pH 6.8 and 0.0005% (w/w) polyethylene glycol-palmitate (a generous gift of Dr G. Johansson [ 151).…”