Specific incorporation of host cell surface proteins into budding vesicular stomatitis virus particles

Lodish, Harvey F.; Porter, Mary

doi:10.1016/0092-8674(80)90397-9

Cited by 72 publications

(50 citation statements)

References 21 publications

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“…In the report by Tan (1975), comparison of Semliki Forest virus, Sindbis virus, Sendai virus, vaccinia virus, rabies virus, vesicular stomatitis virus and pseudorabies virus showed that in most cases there was kinase activity against several different added substrates, again indicating the likely presence of more than one kinase. That some or all of these activities might be of host origin and perhaps related to membrane activities would also be possible given the reported specific incorporation of host cell surface proteins into budding vesicular stomatitis virus particles (Lodish & Porter, 1980). The absolute amounts of these enzymes in the virions are likely to be small and might well have escaped detection as host 'contaminants' of purified virus in previous studies of virion proteins.…”

Section: Discussionmentioning

confidence: 99%

Protein Kinase Activities Associated with the Virions of Pseudorabies and Herpes Simplex Virus

Stevely

Katan

Stirling

et al. 1985

Journal of General Virology

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SUMMARYProtein kinase has been extracted in soluble form from virions of pseudorabies virus using 10~ NP40, 0.6 M-NaC1. Chromatographic analysis of the extract on DEAEcellulose and on phosphocellulose showed it to contain more than one kinase. The activity responsible for the phosphorylation of the major phosphoproteins (mol. wts. 120000, 115000 and 72000) of virions was found to be similar in its properties to the host enzyme casein kinase I1. Purified casein kinase II from ascites cells or from pig liver was able to phosphorylate heat-inactivated virions. In addition to the major phosphoproteins, active virion preparations were able to phosphorylate a minor low molecular weight phosphoprotein, incorporation into which could be stimulated by the addition of cyclic AMP to the assay. Purified host cyclic AMP-dependent protein kinase also phosphorylated this protein in heat-inactivated virions. Analysis of herpes simplex virus type 1 showed that the major phosphoproteins (VP12 and VP23) could be phosphorylated in heat-inactivated virions by added casein kinase II. One of these (VP12) together with a further minor phosphoprotein (VP 14) could be phosphorylated by cyclic AMP-dependent protein kinase.

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Section: Discussionmentioning

confidence: 99%

Protein Kinase Activities Associated with the Virions of Pseudorabies and Herpes Simplex Virus

Stevely

Katan

Stirling

et al. 1985

Journal of General Virology

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“…A long-standing observation has been that molecules from the host cell membrane can be incorporated into viral particles (23). ApoB is an endoplasmic reticulum (ER) membrane-associated protein which initiates VLDL assembly, triglyceride loading in the presence of microsomal triglyceride transfer protein, and VLDL budding into the ER lumen.…”

Section: Discussionmentioning

confidence: 99%

Characterization of Low- and Very-Low-Density Hepatitis C Virus RNA-Containing Particles

André

Komurian-Pradel

Deforges

et al. 2002

J Virol

604

600

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The presence of hepatitis C virus (HCV) RNA-containing particles in the low-density fractions of plasma has been associated with high infectivity. However, the nature of circulating HCV particles and their association with immunoglobulins or lipoproteins as well as the characterization of cell entry have all been subject to conflicting reports. For a better analysis of HCV RNA-containing particles, we quantified HCV RNA in the low-density fractions of plasma corresponding to the very-low-density lipoprotein (VLDL), intermediatedensity lipoprotein, and low-density lipoprotein (LDL) fractions from untreated chronically HCV-infected patients. HCV RNA was always found in at least one of these fractions and represented 8 to 95% of the total plasma HCV RNA. Surprisingly, immunoglobulins G and M were also found in the low-density fractions and could be used to purify the HCV RNA-containing particles (lipo-viro-particles [LVP]). Purified LVP were rich in triglycerides; contained at least apolipoprotein B, HCV RNA, and core protein; and appeared as large spherical particles with a diameter of more than 100 nm and with internal structures. Delipidation of these particles resulted in capsid-like structures recognized by anti-HCV core protein antibody. Purified LVP efficiently bind and enter hepatocyte cell lines, while serum or whole-density fractions do not. Binding of these particles was competed out by VLDL and LDL from noninfected donors and was blocked by anti-apolipoprotein B and E antibodies, whereas upregulation of the LDL receptor increased their internalization. These results suggest that the infectivity of LVP is mediated by endogenous proteins rather than by viral components providing a mechanism of escape from the humoral immune response.

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“…Another explanation is that Thy-1 gp may just accidentally possess a structure to some extent resembling viral gp and enabling an interaction with the M proteins. As already suggested by Lodish & Porter (1980) it will be of great interest to examine cellular proteins which are incorporated into enveloped RNA viruses for a possible structural homology with viral proteins. This may provide insight into the specificity involved in the maturation of these viruses, the ability to exclude certain host surface polypeptides and to incorporate other cellular and viral surface proteins.…”

Section: Discussionmentioning

confidence: 99%

Specific Selection of Host Cell Glycoproteins during Assembly of Murine Leukaemia Virus and Vesicular Stomatitis Virus: Presence of Thy-1 Glycoprotein and Absence of H-2, Pgp-1 and T-200 Glycoproteins on the Envelopes of these Virus Particles

Calafat

Janssen

Démant

et al. 1983

Journal of General Virology

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Specific incorporation of host cell surface proteins into budding vesicular stomatitis virus particles

Cited by 72 publications

References 21 publications

Protein Kinase Activities Associated with the Virions of Pseudorabies and Herpes Simplex Virus

Protein Kinase Activities Associated with the Virions of Pseudorabies and Herpes Simplex Virus

Characterization of Low- and Very-Low-Density Hepatitis C Virus RNA-Containing Particles

Specific Selection of Host Cell Glycoproteins during Assembly of Murine Leukaemia Virus and Vesicular Stomatitis Virus: Presence of Thy-1 Glycoprotein and Absence of H-2, Pgp-1 and T-200 Glycoproteins on the Envelopes of these Virus Particles

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