1990
DOI: 10.1128/iai.58.5.1396-1401.1990
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Specific labeling of cysteine proteinases in pathogenic and nonpathogenic Entamoeba histolytica

Abstract: Growth of Entamoeba histolytica trophozoites was inhibited by 50% at low concentrations (2.0 ,ug/ml) of the diazopeptidyl inhibitor benzyloxycarbonyl-leucyl-L-tyrosyldiazomethane (Z-L-Leu-L-Tyr-CHN2). lodination of the tyrosine residue lowered the growth inhibitory efficacy of the diazopeptidyl inhibitor (50% inhibition, approximately 10 ,ug/ml). However, even at this concentration, practically all of the cysteine proteinase activity of the cells was irreversibly inactivated as shown by fluorescence microscopy… Show more

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Cited by 51 publications
(20 citation statements)
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“…Allicin's antimicrobial properties were suggested to be due to its specific interference with the sulfhydryl enzymes (18). In the current report we present evidence of the remarkable inhibitory effect that pure allicin has on the ability of amebic trophozoites to destroy monolayers of baby hamster kidney (BHK) cells and on the inactivation of the various cysteine proteinases, enzymes which have been shown to be important contributors to amebic virulence and to be important for the degradation of food particles within the amebic vacuoles (5,6,9,15,16).…”
mentioning
confidence: 74%
“…Allicin's antimicrobial properties were suggested to be due to its specific interference with the sulfhydryl enzymes (18). In the current report we present evidence of the remarkable inhibitory effect that pure allicin has on the ability of amebic trophozoites to destroy monolayers of baby hamster kidney (BHK) cells and on the inactivation of the various cysteine proteinases, enzymes which have been shown to be important contributors to amebic virulence and to be important for the degradation of food particles within the amebic vacuoles (5,6,9,15,16).…”
mentioning
confidence: 74%
“…Amoebapore is secreted upon contact with host cells and is lytic at µM concentrations, as determined by increased membrane permeability to vital dyes ( Leippe et al ., 1995 ). Finally, the amoebic cysteine proteinases have been implicated in a cytopathic effect characterized by release of cultured cells from monolayers ( DeMeester et al ., 1990 ; Keene et al ., 1990 ), although partial inhibition of cysteine proteinase production by antisense RNA had no effect ( Ankri et al ., 1998 ; 1999). The parasite's ability to form liver abscesses is impaired in amoebae rendered defective in cytotoxicity, via inducible expression of a dominant negative mutant of the Gal/GalNAc lectin or by expression of antisense amoebapore RNA ( Vines et al ., 1998 ; Bracha et al ., 1999 ).…”
Section: Introductionmentioning
confidence: 99%
“…Cystein proteases are the most abundant proteases present in E histolytica [54,62]. Although several authors [20,59,62] have described a wide variety of proteases with different electrophoretic mobilities, three molecular sizes are the most frequently described: 16 kDa [39], [26][27][28][29]38,59,60,70,77,78] and 56 kDa [20,29,62,70,71]. All of them are inhibited by thiol proteinase inhibitors, especially iodoacetic acid, p.HMB and chicken cystatin, and less effectively by leupeptin, N-ethylmaleimide (NEM), l-chloro-3-tosylamido-7-amino-2-heptanone hydrochloride (TLCK) and antipain [7,8,29,38,39,62,77,78].…”
Section: Cystein Proteasesmentioning
confidence: 99%
“…In our view, the method of Calder6n and Avila [13] preserves more proteins than other methods tested [3,22]. Using sera from recent cases of invasive amebiasis studied by several laboratories in various geographical areas, a basic antigenic pattern of 11 principal proteins with molecular weights of 220, 170, 150, 125,97,80,60,45,20 and 9 kDA was established for the pathogenic E histolytica strain HMI:IMSS, used by most research groups.…”
mentioning
confidence: 99%