1999
DOI: 10.1002/(sici)1097-0061(19990630)15:9<721::aid-yea414>3.0.co;2-w
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Specific negative effects resulting from elevated levels of the recombinational repair protein Rad54p inSaccharomyces cerevisiae

Abstract: RAD54 is an important gene in the RAD52 group that controls recombinational repair of DNA damage in Saccharomyces cerevisiae. Rad54p is a DNA‐dependent ATPase and shares seven conserved sequence motifs with proteins of the Swi2p/Snf2p family. Genetic analysis of mutations in motif IA, the putative ATP‐binding fold of Rad54p, demonstrated the functional importance of this motif. Overexpression of these mutant proteins resulted in strong, dominant‐negative effects on cell survival. High levels of full‐length wil… Show more

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Cited by 37 publications
(19 citation statements)
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“…Thus, the maximal stimulation seen at equimolar amounts of Rad54 and Rad51 proteins in certain assays represents the limiting level rather than an obligatory requirement to form such saturated complexes. In this regard, it remains intriguing that the intracellular ratio of Rad54 protein to Rad51 protein for both yeast and mouse cells is approximately equimolar (46,47), a physiological finding that is consistent with our biochemical observations. The observation that stimulation saturates at lower than equimolar in some in vitro experiments can result from any number of factors.…”
Section: Discussionsupporting
confidence: 89%
“…Thus, the maximal stimulation seen at equimolar amounts of Rad54 and Rad51 proteins in certain assays represents the limiting level rather than an obligatory requirement to form such saturated complexes. In this regard, it remains intriguing that the intracellular ratio of Rad54 protein to Rad51 protein for both yeast and mouse cells is approximately equimolar (46,47), a physiological finding that is consistent with our biochemical observations. The observation that stimulation saturates at lower than equimolar in some in vitro experiments can result from any number of factors.…”
Section: Discussionsupporting
confidence: 89%
“…However, there is no evidence from microarray analyses for increased expression of RAD51 in diploids, and Western blot analysis indicates that there does not appear to be an increase in the Rad51 protein level in haploids expressing both MAT alleles (244). RAD54 present at high copy number also partially suppresses the radiation sensitivity of rad57 mutants, but, like Rad51, Rad54 protein levels do not appear to be affected by MAT heterozygosity (58,244).…”
Section: Rad51 Paralogs (I) Fungal Rad51 Paralogsmentioning
confidence: 97%
“…Rad54 is a critical member of the Rad52 group, and its absence leads to catastrophic sensitivity to double-strand breaks in yeast (2,11,12). This protein displays robust dsDNA-specific ATPase activity, which is essential for its in vivo function (13)(14)(15). Rad54 engages in a specific, albeit transient, interaction with Rad51 protein that leads to stimulation of the Rad51 in vitro recombination activity by Rad54 and the stimulation of the Rad54 ATPase, as well as topological activity by the Rad51 nucleoprotein filament (16)(17)(18)(19)(20)(21)(22)(23).…”
mentioning
confidence: 99%