Specific neonatally-induced tolerance to rat male accessory glands antigens. Transference of specific suppression by spleen mononuclear cells

Yranzo‐Volonté, Nora; Ferro, Maria Elena; Riera, Clelia M.

doi:10.1016/0165-0378(89)90005-3

Cited by 6 publications

(1 citation statement)

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“…Maternal antibodies may be related to this suppression, as anti-MRAG antibody was detected in the offspring before being immunized with MRAG-CFA. 7 Besides, the antigen was detected in the offspring and was transferred via placenta and milk. It is rationally conceivable that immature animals are easily tolerized when they are exposed to extrinsic antigen because it has been demonstrated by many investigators that neonates lack immunocompetence as a result of the functional immaturity of lymphocytea'" and accessory cells.l'' and the existence of large numbers of nonspecific suppressor cella'" in their environment.…”

Section: Discussionmentioning

confidence: 99%

Maternal‐Fetal Interactions on the Regulation of the Autoimmune Response

Yranzo‐Volonté¹,

Riera²

1990

American J Rep Immunol

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Adult female rats were immunized with 5 mg or 25 mg of modified rat male accessory glands (MRAG) incorporated to complete Freund's adjuvant (CFA) before, during, and after pregnancy. The mothers and litters were exchanged between the experimental and normal groups. The offspring were brought up to 20 days of age and immunized with 5 mg of MRAG-CFA and 5 mg of human serum albumin (HSA)-CFA. Anti-MRAG antibodies were detected in the offspring brought up by the immunized mothers and the titers were similar to those of the foster mothers whereas in the offspring of the experimental group fostered by normal mothers antibodies to MRAG were not detected. The DTH performed in the offspring 13 days after the immunization was significantly diminished in male and female offspring from the 5 mg and 25 mg experimental group fostered by normal mothers (P less than 0.0005 for all groups). Similar results were found when the offspring from normal mothers were suckled by mothers immunized with MRAG-CFA. To assess whether MRAG or HSA administered to female rats reached the offspring via the placenta or the milk, female rats were immunized with 3 mg of 125I-MRAG-CFA or with 3 mg of 125I-HSA-CFA. When radioactivity was measured in neonates (n = 11) that were suckled by the 125I-MRAG-CFA immunized mothers, the specific activity was 116 in stomach (0.4 micrograms of MRAG) and 940 in the total organs (3.8 micrograms of MRAG).(ABSTRACT TRUNCATED AT 250 WORDS)

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Section: Discussionmentioning

confidence: 99%

Maternal‐Fetal Interactions on the Regulation of the Autoimmune Response

Yranzo‐Volonté¹,

Riera²

1990

American J Rep Immunol

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Antigen‐induced inhibition of autoimmune response to rat male accessory glands: distinct role of I‐A and I‐E‐positive peritoneal cells

1990

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The present report describes the structural and functional characteristics of the population of peritoneal cells (PC) from rats injected intraperitoneally (i.p.) 2 h or 24 h previously with a suppressor dose of a purified fraction (FI) of rat male accessory glands (RAG: FI-PC2h and FI-PC24h, respectively). Both populations of PC showed the presence of the autoantigens of RAG on their membrane. The study of cell surface marker showed increase of OX17 (I-E) in FI-PC2h and OX6 (I-A) in FI-PC24h. I.p. injection of FI-PC2h 10 and 3 days prior to immunization with chemically modified (diazotized arsanilic and sulfanilic acid) RAG (MRAG) in complete Freund's adjuvant induced suppression of the delayed-type hypersensitivity (DTH) response to MRAG, whereas the i.p. injection of FI-PC24h induced potentiation of DTH response to MRAG when compared with controls (animals injected 10 and 3 days prior to immunization with PC from rats injected i.p. with an unrelated antigen). The treatment of FI-PC2h with OX17 and FI-PC24h with OX6 prior to the transfer blocked the suppression and potentiation described above. These findings show that the i.p. injection of an FI of RAG at a suppressor dose induces PC with immunologically opposite characteristics, depending on the time of obtention.

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