Specific polyclonal and monoclonal antibody prevents paraquat accumulation into rat lung slices

Wright, Andrew Martin; Green, Tim P.; Robson, Richard T.; Niewola, Zbigniew; Wyatt, I. J.; Smith, Lewis L.

doi:10.1016/0006-2952(87)90089-x

Cited by 18 publications

(2 citation statements)

References 18 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Previous work has been concentrated on biochemical, molecular biological, and in some cases on conventional histological techniques for characterization of explant metabolism (Fisher and Placke 1987;Kinnard et al 1994;Shapiro et al 1997;Wright et al 1987). The embedding of cultured slices allowed us to cut more than 20-50 paraffin sections from a single slice for histological and immunohistological evaluation.…”

Section: Discussionmentioning

confidence: 99%

Early signs of lung fibrosis after in vitro treatment of rat lung slices with CdCl2 and TGF-?1

Kasper

Seidel

Knels

et al. 2004

Histochemistry and Cell Biology

View full text Add to dashboard Cite

Precision-cut rat lung slices have been employed in combination with an extensive immunohistochemistry of paraffin-embedded slices for monitoring of early pathohistological changes after exposure to CdCl(2)/TGF-beta(1). Three days of CdCl(2) exposure in combination with TGF-beta(1) seem to be sufficient to induce lung injury with alterations similar to changes observed in early lung fibrogenesis: (1) extracellular matrix accumulation and myofibroblast transdifferentiation (Sirius red staining, collagen type IV, alpha-smooth muscle actin), (2) type I cell injury with loss of type I cell antigens (T1alpha antigen, aquaporin-5, RAGE), (3) increased apoptosis of pulmonary cells (active caspase-3, vimentin cleavage product V1 of caspase-9), and (4) activation of microvascular endothelial cells (podocalyxin, caveolin-1). Western blot analysis confirmed the increasing amount of alpha-smooth muscle actin, the loss of T1alpha antigen, and the increase in caveolin-1 immunoreactivity. The explant culture using CdCl(2)/TGF-beta(1) provides a suitable tool for the study of other factors involved in pulmonary pathology including transcription factors, cytokines, and other metabolites involved in early stages of fibrogenesis.

show abstract

Section: Discussionmentioning

confidence: 99%

Early signs of lung fibrosis after in vitro treatment of rat lung slices with CdCl2 and TGF-?1

Kasper

Seidel

Knels

et al. 2004

Histochemistry and Cell Biology

View full text Add to dashboard Cite

show abstract

“…Lung slice organ cultures have been prepared from many different animals, including mice, rats, hamsters, and cattle 6,7,14 . We have pioneered the use of human lung slices to study the pathophysiology of innate immune responses to infectious agents, including adenovirus (Ad), influenza virus, and the aetiological agent of anthrax, Bacillus anthracis [15][16][17][18] .…”

Section: History Of the Development Of Lung Tissue Modelsmentioning

confidence: 99%