Specific procedure for analysing steryl glucosides in olive oil

Gómez‐Coca, Raquel B.; Pérez‐Camino, María del Carmen; Moreda, Wenceslao

doi:10.1002/ejlt.201200181

Cited by 14 publications

(12 citation statements)

References 35 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The exclusion of acid hydrolysis may lead to a severe underestimation of the total sterol content, because in certain foods the content of glycosylated sterols can even exceed FS and SE content [17]. Alternatively, SG can be directly analyzed by GC as a trimethylsilyl ether of the intact compound, thereby avoiding acid-catalyzed isomerization [18][19][20][21]. As a drawback, this method depends on external calibration due to the lack of a suitable internal standard, and complete derivatization of all hydroxyl groups remains a critical step.…”

Section: Introductionmentioning

confidence: 99%

Comparison of Enzymatic Hydrolysis and Acid Hydrolysis of Sterol Glycosides from Foods Rich in Δ⁷‐Sterols

Münger

Jutzi

Lampi

et al. 2015

Lipids

View full text Add to dashboard Cite

In this study, we present the difference in sterol composition of extracted steryl glycosides (SG) hydrolyzed by either enzymatic or acid hydrolysis. SG were analyzed from foods belonging to the plant families Cucurbitaceae (melon and pumpkin seeds) and Amaranthaceae (amaranth and beetroot), both of which are dominated by Δ(7)-sterols. Released sterols were quantified by gas chromatography with a flame ionization detector (GC-FID) and identified using gas chromatography/mass spectrometry (GC-MS). All Δ(7)-sterols identified (Δ(7)-stigmastenyl, spinasteryl, Δ(7)-campesteryl, Δ(7)-avenasteryl, poriferasta-7,25-dienyl and poriferasta-7,22,25-trienyl glucoside) underwent isomerization under acidic conditions and high temperature. Sterols with an ethylidene or methylidene side chain were found to form multiple artifacts. The artifact sterols coeluted with residues of incompletely isomerized Δ(7)-sterols, or Δ(5)-sterols if present, and could be identified as Δ(8(14))-sterols on the basis of relative retention time, and their MS spectra as trimethylsilyl (TMS) and acetate derivatives. For instance, SG from melon were composed of 66% Δ(7)-stigmastenol when enzymatic hydrolysis was performed, whereas with acid hydrolysis only 8% of Δ(7)-stigmastenol was determined. The artifact of Δ(7)-stigmastenol coeluted with residual non-isomerized spinasterol, demonstrating the high risk of misinterpretation of compositional data obtained after acid hydrolysis. Therefore, the accurate composition of SG from foods containing sterols with a double bond at C-7 can only be obtained by enzymatic hydrolysis or by direct analysis of the intact SG.

show abstract

Section: Introductionmentioning

confidence: 99%

Comparison of Enzymatic Hydrolysis and Acid Hydrolysis of Sterol Glycosides from Foods Rich in Δ⁷‐Sterols

Münger

Jutzi

Lampi

et al. 2015

Lipids

View full text Add to dashboard Cite

show abstract

“…(C)), but mass spectra with ammonium ([M + NH 4 ] + ) and sodium ([M + Na] + ) adduct ions of seven molecular species were obtained (Table ). By referring to known information in previous lecithin papers, four sterylglucosides containing sitosterol‐3 β ‐glucoside, campesteryl‐3 β ‐glucoside, stigmasteryl‐3 β ‐glucoside, and brassicasteryl‐3 β ‐glucoside were confirmed to be the dominating compositions in fraction 3 . One GluCer with hydroxy stearic acid and sphingosine, one glucose ester, and one monogalactosyldiacylglycerol (C18:3/C18:3) were clearly detected in this mass spectrometry analysis system.…”

Section: Resultsmentioning

confidence: 59%

“…By referring to known information in previous lecithin papers, 1,2,39,40 four sterylglucosides containing sitosterol-3 -glucoside, campesteryl-3 -glucoside, stigmasteryl-3 -glucoside, and wileyonlinelibrary.com/jsfa brassicasteryl-3 -glucoside 33 were confirmed to be the dominating compositions in fraction 3. 32,38,41,42 One GluCer 42-45 with hydroxy stearic acid and sphingosine, one glucose ester, and one monogalactosyldiacylglycerol (C18:3/C18:3) were clearly detected in this mass spectrometry analysis system.…”

Section: Uplc-q-tof-esi-ms Identificationmentioning

confidence: 85%

Molecular species determination of oligosaccharides and glycoconjugates in soybean lecithin powders

2018

View full text Add to dashboard Cite

show abstract

“…In this article, high‐performance liquid chromatography (HPLC) instead of gas chromatography mass spectrometry (GC‐MS) was applied to detect ASG and SG content in PG extract without sterol derivatization . Thus, the construction of the evaluation system for PG extraction was mainly operated by principle component analysis (PCA) in SIMICA software.…”

Section: Introductionmentioning

confidence: 99%

Evaluation system construction and factor impact analysis of silica‐gel adsorption to extract phytosterol glycosides from soybean lecithin powder

Kang

Che

et al. 2019

J Sci Food Agric

View full text Add to dashboard Cite

BACKGROUND: Soybean lecithin powders are good sources of phytosterol glucosides (PGs) containing acyl-sterylglycosides (ASGs) and sterylglucosides (SGs), but PG extraction from soybean lecithin powder is difficult due to the solubilizing property of phospolipids. To comprehensively utilize soybean lecithin resources, an evaluation system construction and factor impact analysis of PG extraction by silica-gel adsorption was investigated in this article. RESULTS:With high-performance liquid chromatography (HPLC) as the main experimental analysis method, software such as SIMICA and SPSS were applied to construct an evaluation system of PG extraction. Different from scores plot in SIMICA for distinguishing samples in chloroform from others, the loading plot and binary variant correlation analysis of all indicators in PG extraction were brought to confirm four evaluation indicators containing PG purity, ASG recovery, SG recovery and phospholipid recovery. In the factor impact analysis, four times elution from silica-gel sediment was enough to achieve a PG product with least reagent waste, while SPW in petroleum ether at 50 mg mL −1 with 1:3 silica-gel dosage (lecithin/silica-gel, w/w) was then determined as the optimum of single factors. CONCLUSION: All studies in this article were of great significance, as they laid foundations for research of PG extraction procedure, as well as PG industrial production, facilitating the comprehensive utilization of lecithin resources.

show abstract

Specific procedure for analysing steryl glucosides in olive oil

Cited by 14 publications

References 35 publications

Comparison of Enzymatic Hydrolysis and Acid Hydrolysis of Sterol Glycosides from Foods Rich in Δ⁷‐Sterols

Comparison of Enzymatic Hydrolysis and Acid Hydrolysis of Sterol Glycosides from Foods Rich in Δ⁷‐Sterols

Molecular species determination of oligosaccharides and glycoconjugates in soybean lecithin powders

Evaluation system construction and factor impact analysis of silica‐gel adsorption to extract phytosterol glycosides from soybean lecithin powder

Contact Info

Product

Resources

About

Specific procedure for analysing steryl glucosides in olive oil

Cited by 14 publications

References 35 publications

Comparison of Enzymatic Hydrolysis and Acid Hydrolysis of Sterol Glycosides from Foods Rich in Δ7‐Sterols

Comparison of Enzymatic Hydrolysis and Acid Hydrolysis of Sterol Glycosides from Foods Rich in Δ7‐Sterols

Molecular species determination of oligosaccharides and glycoconjugates in soybean lecithin powders

Evaluation system construction and factor impact analysis of silica‐gel adsorption to extract phytosterol glycosides from soybean lecithin powder

Contact Info

Product

Resources

About

Comparison of Enzymatic Hydrolysis and Acid Hydrolysis of Sterol Glycosides from Foods Rich in Δ⁷‐Sterols

Comparison of Enzymatic Hydrolysis and Acid Hydrolysis of Sterol Glycosides from Foods Rich in Δ⁷‐Sterols