Specific production of eosinophil colony stimulating factor from sensitized T cells from a patient with allergic eosinophilia

Abstract

To explore the possibility that an eosinophil colony stimulating factor (EO-CSF) is elaborated independently of neutrophil CSF (N-CSF), we compared the effect on the production of EO-CSF and N-CSF of adding a specific antigen, an aspergillus extract, to peripheral blood leucocytes of an eosinophilic patient with allergic bronchopulmonary aspergillosis. Conditioned media prepared from the patient's mononuclear (MN) and T cells were assayed for EO-CSF and N-CSF activities by agar culture technique, using normal human nonphagocytic MN bone marrow cells as target cells. The addition of the specific antigen to the cultures of the patient's MN or T cells significantly stimulated the production of EO-CSF, but not that of N-CSF, while the patient's non-T cells and normal MN or T cells were not stimulated by the antigen challenge to produce either CSF. These results suggest that EO-CSF is a factor distinct from N-CSF, that its production is dependent on the presence of sensitized T cells with antigen-specific stimulation, and that it might be one of the causes of blood eosinophilia in this patient.

Mast cells and pathological process in lungs of rats and mice

“…Cytokines, chemokines, inflammatory mediators produced by phagocytes attract more inflammatory cells from blood as well as from tissues (mast cells) (4,6,10,44). Involvement of many inflammatory cells, their interaction, releasing of some proteolytic enzymes (collagenase, elastase), harmfull effect of pathogens causes a complicated mechanism that damage lung tissue (7,12,30). Bronchiectasis was much more pronounced in rats than in mice.…”

“…These cells release large amont of mediators: TGF-β, basic fibroblast growth factors, platelet-derived growth factor, matrix metalloproteases, vascular endothelial growth factors, nerve growth factors, neuropeptides, cytokines such as IL-1β and IL-6 (7,12,20,24,30,36,43). Eosinophils also release proteins, eicosanoids, leukotrienes, reactive oxygen species, and cell-cell signaling molecules (7,10,12,20,41). These cells are related to pathological processes such as fibrosis, vascular leakage, angiogenesis, epithelial desquamation, epithelial metaplasia, smooth muscle hypertrophy (20) and with normal processes as bone metabolism, tissue repair/remodelation (367).…”

Malignant Fibrous Histiocytoma Associated with Peripheral Blood Eosinophilia

“…The proliferation and differentiation of every single hemopoietic progenitor cell is controlled by different humoral regulatory substances derived from various cellular sources [12 14], In the case of the CFU-Eos the corresponding regulator is a putative eosinophilopoietin, or the colony-stimulating factor for eosinophils [15]. This eosinophilopoietin has been shown to be produced by trichinella-sensitized lym phocytes [16], by aspcrgillus-scnsitized lymphocytes [17] and by normal spleen cells [15], too. Although many tumor cell lines have been shown to produce colony-stimulating activities upon hemopoctic stem cells [18,19], it has been shown only recently that a peripheral blood cosinophilia in a cancer patient was caused by a tumor-derived eosinophilopoietin with in vitro activity upon CFU-Eos [4].…”

NERDS Syndrome: An Additional Case Report

“…The direct inter action and the production of eosinophilic chemotactic fac tors and interleukins by T lymphocytes [17], suggest a cen tral role of T lymphocytes.…”