Specific qPCR assays for the detection of orf virus, pseudocowpox virus and bovine papular stomatitis virus

Wilkins, Kimberly; Damon, Inger K.; Yu, Li

doi:10.1016/j.jviromet.2013.08.027

Cited by 37 publications

(27 citation statements)

References 27 publications

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“…A real-time PCR assay that targets the orf virus gene J6R was performed on DNA [4]. Reactions were performed in 25-µL volumes on an ABI7900 instrument (Applied Biosystems, Foster City, California).…”

Section: Methodsmentioning

confidence: 99%

Unique Presentation of Orf Virus Infection in a Thermal-Burn Patient After Receiving an Autologous Skin Graft

et al. 2016

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We describe a burn patient who developed skin lesions on her skin-graft harvest and skin-graft recipient (burn) sites. Orf virus infection was confirmed by a combination of diagnostic assays, including molecular tests, immunohistochemical analysis, pathologic analysis, and electron microscopy. DNA sequence analysis grouped this orf virus isolate among isolates from India. Although no definitive source of infection was determined from this case, this is the first reported case of orf virus infection in a skin graft harvest. Skin graft recipients with exposures to animals may be at risk for this viral infection.

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Section: Methodsmentioning

confidence: 99%

Unique Presentation of Orf Virus Infection in a Thermal-Burn Patient After Receiving an Autologous Skin Graft

et al. 2016

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“…Orf can cause high morbidity in adults and high mortality in lambs and kids (up to 90%), while Capripoxviruses can cause high mortality and morbidity . A digital droplet PCR detection of ORFV, pseudocowpox virus (PCPV), and bovine papular stomatitis virus (BPSV) using RNA polymerase gene sequences has also been developed …”

Section: Laboratory Testingmentioning

confidence: 99%

“…Orf is an epitheliotropic virus that causes a highly contagious vesiculoulcerative pustular infection of both keratinized skin and mucosal surfaces. 1,24 The most important predisposing factor is loss of epithelial barrier integrity. 2 It affects damaged skin as it requires abrasions and breaks in the skin for infection.…”

Section: Clinical Featuresmentioning

confidence: 99%

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Orf virus infection

Bergqvist

Kurban

Abbas

2017

Reviews in Medical Virology

101

117

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Orf virus (ORFV) is an important pathogen responsible for a highly contagious zoonotic viral infection that threatens those who handle sheep and goats. Orf virus is the prototype of the Parapoxvirus genus, and its resilience in the environment and ability to reinfect its host has contributed to the spread and maintenance of the infection in many species. In healthy humans, the disease usually resolves spontaneously within 3 to 6 weeks. There is no specific treatment and many different approaches such as use of imiquimod, cidofovir, curettage, shave excision, cryotherapy, and electrocautery have all been reported to be successful, without supporting evidence from controlled clinical trials. Throughout its interaction with the different hosts, ORFV has evolved a strategy for immune evasion via the development of an array of virulence factors. The interaction of ORFV with the immune system has been the subject of research for decades. Whole inactivated ORFV has been used as a type of immunomodulating drug; a so called paramunity inducer proposed as both a preventative and a therapeutic immunomodulator across various species. Additional research on the remarkable strategies underlying ORFV infection could lead to improved understanding of skin immunity.

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“…7 Negative results allowed us to continue the work with lower biosecurity when handling samples during searches for other agents. The differential diagnosis process continued with the following molecular tests: BVDV, 6,25 VSV (protocol used for detection subtypes Alagoas, Indiana, New Jersey, and Cocal), 29 OvHV-2 and BTV, 18,30 orthopoxvirus (OPV), 13 PPV, 37 and BoHV-1. 33 All PCR assays were accompanied by blank controls to evaluate contamination.…”

Section: Molecular Analysismentioning

confidence: 99%

Detection of multiple viral infections in cattle and buffalo with suspected vesicular disease in Brazil

Laguardia-Nascimento¹,

Sales²,

Gasparini³

et al. 2016

J VET Diagn Invest

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Abstract. Vesicular diseases are of high importance for livestock, primarily because of foot-and-mouth disease (FMD), which is a high-morbidity disease that generates direct losses caused by low milk production, weight loss, and indirect losses because of the need for sanitary barriers. Other vesicular diseases are also of importance for livestock because of direct impacts or because their clinical signs may be confused with those of FMD. We report herein the detection of multiple infections in cattle with suspected vesicular disease in the Brazilian states of Amazonas (AM), Mato Grosso (MT), and Roraima. Thirtyseven epithelial samples from cattle and 1 sample from a buffalo were sent to the laboratory for testing for FMDV and similar disease agents. All samples from MT were positive for parapoxvirus (Pseudocowpox virus and Bovine papular stomatitis virus). In addition, 3 samples were positive for Bluetongue virus, and 5 samples were positive for Bovine herpesvirus 1. Among these samples, 1 was positive for all of these 3 agents. Only 2 samples from AM were negative for parapoxvirus. The molecular tests conducted in this study detected multiple infections, with a high prevalence of parapoxvirus.

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Specific qPCR assays for the detection of orf virus, pseudocowpox virus and bovine papular stomatitis virus

Cited by 37 publications

References 27 publications

Unique Presentation of Orf Virus Infection in a Thermal-Burn Patient After Receiving an Autologous Skin Graft

Unique Presentation of Orf Virus Infection in a Thermal-Burn Patient After Receiving an Autologous Skin Graft

Orf virus infection

Detection of multiple viral infections in cattle and buffalo with suspected vesicular disease in Brazil

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