Specific Strains of Lactic Acid Bacteria Differentially Modulate the Profile of Adipokines In Vitro

Abstract

Obesity induces local/systemic inflammation accompanied by increases in macrophage infiltration into adipose tissue and production of inflammatory cytokines, chemokines, and hormones. Previous studies have shown that probiotics could improve the intestinal dysbiosis induced by metabolic diseases such as obesity, diabetes, and metabolic syndrome. Microorganisms could (directly or indirectly) affect adipokine levels due to their capacity to induce translocation of several intestinal microbial antigens into systemic circulation, which could lead to metabolic endotoxemia or produce immunomodulation in different organs. The aim of the present study was to select non-inflammatory lactic acid bacteria (LAB) strains with the capacity to modulate adipokine secretion by the adipose tissue. We wish to elucidate the role of potential probiotic strains in the regulation of the cross talking between immune cells such as macrophages and adipose cells. Mouse macrophage cell line RAW 264.7 was used for evaluating the ability of 14 LAB strains to induce cytokine production. The LAB strains were chosen based on their previously studied beneficial properties in health. Then, in murine adipocyte culture and macrophage–adipocyte coculture, we determined the ability of these strains to induce cytokines and leptin secretion. Tumor necrosis factor alpha, interleukin 6 (IL-6), IL-10, monocyte chemoattractant protein-1, and leptin levels were measured in cell supernatants. We also performed the detection and quantification of leptin receptor (Ob-Rb) expression in macrophage cell lines stimulated by these LAB strains. Differential secretion profile of cytokines in macrophage cells induced by LAB strains was observed. Also, the levels of Ob-Rb expression diverged among different LAB strains. In LAB-stimulated coculture cells (adipocytes and macrophages), we observed differential production of leptin and cytokines. Furthermore, we detected lower production levels in single culture than cocultured cells. The principal component analysis showed an association between the four clusters of strains established according to their inflammatory profiles and leptin adipocyte production and leptin receptor expression in macrophages. We conclude that coculture is the most appropriate system for selecting strains with the ability to modulate adipokine secretion. The use of microorganisms with low and medium inflammatory properties and ability to modulate leptin levels could be a strategy for the treatment of some metabolic diseases associated with dysregulation of immune response.

Effects of a 6-month multi-strain probiotics supplementation in endotoxemic, inflammatory and cardiometabolic status of T2DM patients: A randomized, double-blind, placebo-controlled trial

“…Previous observations have suggested that endotoxins from non-commensal bacteria may affect adipocytokine levels secondary to translocation induction of several intestinal microbial antigens into the circulation, creating an altered adipokine profile and intestinal dysbiosis [34]. Certain probiotics, specifically lactic acid bacteria strains, have demonstrated in vitro that they can differentially modulate adipokine expression and the inflammatory response [35]. It is noteworthy that 6 of the 8 probiotic strains used in this study belong to the lactic acid bacteria class.…”

A soy-based probiotic drink modulates the microbiota and reduces body weight gain in diet-induced obese mice

“…of TNF-α, IL-6, IL-10 and MCP-1 in adipocytes. Lactococcus lactis CRL1434 strain increases TNF-α, MCP-1, and IL-10 expression in comparison to other strains, while the Lactobacillus plantarum CRL350 strain induced higher levels of IL-6 (Fabersani et al, 2017). IL-6 is secreted by adipocytes primarily in sites that present with acute or chronic inflammation, such as by the visceral adipose tissue (Castoldi, De Souza, Saraiva Câmara, & Moraes-Vieira, 2016).…”

New Progress Regarding the Use of Lactic Acid Bacteria as Live Delivery Vectors, Treatment of Diseases and Induction of Immune Responses in Different Host Species Focusing on Lactobacillus Species

“…Different probiotic strains of Lactobacillus genera acquire significant and widely acknowledged immunomodulatory properties and health-promoting [35,138]. The approaches leading to the efficient targetness engages the natural ways which do not cause any side effects and assist the body to uphold the immune homeostasis such as the use of probiotics are the ways forward.…”

“…The market size of probiotics has to the highest degree increased consumer anxiety concerning health-promoting effect of nutraceuticals [38,138,151].…”