Specific stress-induced storage of trehalose, glycerol and d-arabitol in response to oxidative and osmotic stress in Candida albicans

Sánchez-Fresneda, Ruth; Guirao-Abad, José P.; Argüelles, Alejandra; Gónzalez-Párraga, Pilar; Valentı́n, Eulogio; Argüelles, Juan-Carlos

doi:10.1016/j.bbrc.2012.10.118

Cited by 51 publications

(38 citation statements)

References 20 publications

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“…The increase in glycerol concentration in the silage without glycerine and in the silage treated with 4% glycerine may be the result of yeast metabolism. Yeasts produce glycerol during alcoholic fermentation (Smidt et al ., ), and also osmotic changes induce the significant storage of glycerol in yeasts (Sánchez‐Fresneda et al ., ). In the silage treated with 8 and 12% glycerine, the reduction in glycerol concentration after fermentation is possibly due to effluent loss.…”

Section: Discussionmentioning

confidence: 97%

Methylotrophic yeast, lactic acid bacteria and glycerine as additives for sugarcane silage

Carvalho

Ávila

Pereira

et al. 2016

Grass and Forage Science

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The ensiling of sugarcane results in high dry‐matter (DM) loss, but the addition of glycerine may compensate for the loss during ensiling. Methanol is the most undesirable contaminant of crude glycerine destined for animal feeding. The aim of this study was to evaluate the ability of the yeast strain Pichia methanolica NCYC 1381 to reduce the methanol concentration in sugarcane silage inoculated with Lactobacillus hilgardii CCMA 0170 + glycerine. A randomized design consisted of four dose rates of glycerine (0, 4, 8 and 12% of fresh forage), three periods of silage fermentation (11, 34 and 68 d) and three combinations of microbial additives [L. hilgardii (LH), L. hilgardii plus P. methanolica (LH + PM) and without microbial additive (WI)]. The DM of the fresh sugarcane was 275 g kg−1. The linear reduction in neutral detergent fibre caused by glycerine inclusion was probably due to a dilution effect. The LH treatment increased the concentrations of the succinic, acetic and propionic acids, and 1,2‐propanediol, and reduced the yeast population. The LH + PM treatment increased DM loss of sugarcane silage with 12% glycerine and L. hilgardii CCMA 0170 (6·1 log cfu g−1 of FM) reduced the DM loss when compared to the silage without additives. Under the conditions of the experiment, the P. methanolica treatment did not reduce the methanol concentration in silage.

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Section: Discussionmentioning

confidence: 97%

Methylotrophic yeast, lactic acid bacteria and glycerine as additives for sugarcane silage

Carvalho

Ávila

Pereira

et al. 2016

Grass and Forage Science

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“…In C. albicans and a number of yeasts, one strategy to counteract oxidative and osmotic stresses is the rapid endogenous synthesis of compatible solutes or, under exposure to cell wall stresses, cell wall biogenesis [37, 38]. These stress responses which are directly or indirectly linked to glucose metabolism, could have been affected in the hexose kinase mutants.…”

Section: Resultsmentioning

confidence: 99%

Hexokinase and glucokinases are essential for fitness and virulence in the pathogenic yeastCandida albicans

Laurian

Dementhon

Doumèche

et al. 2018

Preprint

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Metabolic flexibility promotes infection and commensal colonization by the opportunistic pathogen Candida albicans. Yeast cell survival depends upon assimilation of fermentable and non-fermentable locally available carbon sources. Physiologically relevant sugars like glucose and fructose are present at low level in host niches. However, because glucose is the preferred substrate for energy and biosynthesis of structural components, its efficient metabolization is fundamental for the metabolic adaptation of the pathogen. We explored and characterized the C. albicans hexose kinase system composed of one hexokinase (CaHxk2) and two glucokinases (CaGlk1 and CaGlk4). Using a set of mutant strains, we found that hexose phosphorylation is mostly assured by CaHxk2, which sustains growth on hexoses. Our data on hexokinase and glucokinase expression point out an absence of cross regulation mechanisms at the transcription level and different regulatory pathways. In the presence of glucose, CaHxk2 migrates in the nucleus and contributes to the glucose repression signaling pathway. In addition, CaHxk2 participates to oxidative, osmotic and cell wall stress responses, while glucokinases are overexpressed under hypoxia. Hexose phosphorylation is a key step necessary for filamentation, that is affected in the hexokinase mutant. Virulence of this mutant is clearly impacted in the Galleria mellonella and macrophage models. Filamentation, glucose phosphorylation and stress response defects of the hexokinase mutant prevent host killing by C. albicans. By contributing to metabolic flexibility, stress answer response and morphogenesis, hexose kinase enzymes play an essential role in the virulence of C. albicans.Author summaryThe pathogenic yeast C. albicans is both a powerful commensal and pathogen of humans that can infect wide range of organs and body sites. To grow in its host and establish an infection, the pathogen must assimilate carbon from these heterogenous environments. C. albicans regulates central carbon metabolism in a niche-specific manner, activating alternatively gluconeogenesis, glyoxylate cycle and the glycolytic metabolism. For yeast and other microorganisms, glucose is the preferred carbon and energy source and its accurate detection and metabolism is essential. However, the glycolytic hexose kinase system has not been investigated yet in C. albicans. In this report, we showed that hexokinase and glucokinases contribute to the fitness and virulence of C. albicans. We revealed the main metabolic role of the hexokinase CaHxk2 which impacts on growth, glucose signalling, morphological transition and virulence. However, glucokinases contribute to the anoxic response and their implication in regulation processes is suggested.

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“…The authors indicated that glutathione reductase can be inhibited by trehalose in a dose-dependent manner. On the other hand, Sanchez-Fresneda et al (33) reported that the stress-induced trehalose accumulation is Hog1-independent in Candida albicans. The extension of lifespan in S. pombe is associated with both calorie restriction and increased resistance to oxidative stress (10)(11)(12).…”

Section: Discussionmentioning

confidence: 99%

Role of Oxidative Stress Response and Trehalose Accumulation in the Longevity of Fission Yeast

Palabıyık

Ghods

2015

Jundishapur J Microbiol

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Background:Glucose is the preferred carbon and energy source in most organisms and plays an active role in the regulation of many biological processes. However, an excess of glucose leads to such undesirable conditions as diabetes and age-related diseases. Since Schizosaccharomyces pombe homologous of many human genes, it offers several advantages for the investigation of the molecular mechanisms underlying human disease and aging studies. We have identified two glucose-repression-resistant mutants (ird5 and ird11) of S. pombe. Objectives: We aimed to investigate the possible relationship between lifespan extension and oxidative stress response induced by exposure to hydrogen peroxide alongside the trehalose accumulation level by using the two S. pombe mutants (i.e. ird5 and ird11), which are repressed by glucose and are resistant to oxidative stress. Materials and Methods:We employed trehalose accumulation measurement and colony-forming unit (CFU) counting using the ird mutants in exponential and stationary phases and compared them to the wild type grown in repressed, de-repressed, and stressed conditions to clarify the possible relationship between glucose signaling, oxidative stress response, and lifespan in S. pombe. Results: The lifespan of the ird5 mutant was significantly longer that of either the ird11 mutant or the wild type cells. Under repressed condition, the trehalose content was increased remarkably on the 3rd day of the study in the ird11 mutant and the wild type. Under de-repressed condition, the level of intracellular trehalose was notably increased on the 3rd day in ird11. Under stressed condition, the trehalose level in ird11 was increased on the 3rd day as a pattern similar to that observed in the wild type. Conclusions: Our results demonstrated no significant correlation between the ird5 lifespan and the trehalose concentration. Likewise, the correlation between lifespan extension, trehalose accumulation, and cellular resistance to hydrogen peroxide was not significant.

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Specific stress-induced storage of trehalose, glycerol and d-arabitol in response to oxidative and osmotic stress in Candida albicans

Cited by 51 publications

References 20 publications

Methylotrophic yeast, lactic acid bacteria and glycerine as additives for sugarcane silage

Methylotrophic yeast, lactic acid bacteria and glycerine as additives for sugarcane silage

Hexokinase and glucokinases are essential for fitness and virulence in the pathogenic yeastCandida albicans

Role of Oxidative Stress Response and Trehalose Accumulation in the Longevity of Fission Yeast

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