Specific tail swelling pattern in hypo-osmotic solution as a predictor of DNA fragmentation status in human spermatozoa

Abstract

ObjectiveThe aim of this study was to investigate DNA fragmentation status in human spermatozoa according to specific tail swelling patterns determined via hypo-osmotic swelling test (HOST).MethodsFrozen semen samples from 21 healthy donors were thawed and prepared by the swim-up technique for use in intracytoplasmic sperm injection. The semen samples were treated for 5 minutes as part of the HOST procedure and then underwent the sperm chromatin dispersion test using a Halosperm kit. DNA fragmentation status (large halo, medium halo, small halo, no halo, or degraded) and the specific tail swelling pattern (“a”–“g”) were assessed at the level of a single spermatozoon. A total of 42,000 spermatozoa were analyzed, and the percentage of spermatozoa without DNA fragmentation (as evidenced by a large or medium halo) was assessed according to the specific tail swelling patterns observed.ResultsThe HOST examinations showed that >93% of spermatozoa across all types displayed no DNA fragmentation. The percentage of spermatozoa without DNA fragmentation was 100% in type “d”, 98.67% in type “g”, and 98.17% in type “f” spermatozoa.ConclusionWe found that the type “d” spermatozoa displayed no DNA fragmentation, but the other types of spermatozoa also displayed very low rates of DNA fragmentation. This result may be associated with the processing of the spermatozoa by density gradient centrifugation and the swim-up technique.

A Case Study on the Efficacy of Hypo-Osmotic Swelling Test and Assisted Hatching in Overcoming Necrozoospermia Challenges

“…The sperm tails in a hypo-osmotic solution might or might not swell during the incubation of semen samples in the solution. The WHO's Fifth Manual states that these conditions are classified as necrozoospermia if the percentage of live cells is less than 58% [ 7 , 8 ].…”

The Author Reply: Effect of Sperm DNA Fragmentation on Embryo Quality in Normal Responder Women in IVF and ICSI

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Curso temporal dos efeitos do infravermelho longo sobre o desempenho dos músculos extensores e flexores do joelho e fertilidade masculina

“…AGARWAL et al, 2017;ALARGKOF et al, 2019;CANKUT et al, 2019;COLACO;SAKKAS, 2018;FAINBERG;KASHANIAN, 2019;KIM et al, 2019;ALVAREZ, 2010). A PBMT ao não afetar a integridade do DNA do esperma e ao mesmo tempo melhorar outros aspectos do sêmen (e.g., motilidade, modulação de Ca 2+ ) favorece o tratamento de quadros de infertilidade masculina, pois os espermatozoides possuem uma capacidade reduzida de reparo(AGARWAL et al, 2017;ELBARDISI et al, 2020;JOHNSON et al, 2015).…”