Specificities of Prostaglandins B1, F1α, and F2α Antigen-Antibody Reactions

Levine, Lawrence; Cernosek, R.M. Gutierrez; Vunakis, Helen Van

doi:10.1016/s0021-9258(19)45913-9

Cited by 247 publications

(4 citation statements)

References 6 publications

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“…Antiserum. Preparation and specificities of the monkey antibodies to 15-keto-PGF2a' and rabbit antibodies to PGF2" have been described (Levine and Gutierrez-Cernosek, 1972 and Levine et al, 1971).…”

Section: Methodsmentioning

confidence: 99%

See 1 more Smart Citation

Distribution of prostaglandin E9-ketoreductase and types I and II 15-hydroxyprostaglandin dehydrogenase in swine kidney medulla and cortex

Sc¹,

Ss²,

Katzen³

et al. 1975

Biochemistry

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Prostaglandin E 9-ketoreductase, NAD+-dependent 15-hydroxyprostaglandin dehydrogenase (type I), and NADP+-dependent 15-hydroxyprostaglandin dehydrogenase (type II) have been partially purified from swine renal medulla and cortex. Eleven times more NAD+-dependent 15-hydroxyprostaglandin dehydrogenase activity was found in the cortex than in the medulla. On the other hand, about twice as much NADP+-dependent dehydrogenase activity was found in the medulla than in the cortex. The prostaglandin 9-ketoreductase activities were equally distributed in the swine kidney cortex and medulla.

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Section: Methodsmentioning

confidence: 99%

“…The precipitate was dissolved in 0.2 ml of 0.1 N NaOH and counted for radioactivity in a modified Bray's solution with a Packard liquid scintillation spectrometer. For analysis of PGF2a, diluted rabbit antiserum and goat-antirabbit-7-globulin were used for radioimmunoassay by the double antibody procedure (Levine et al. 1971).…”

mentioning

confidence: 99%

Distribution of prostaglandin E9-ketoreductase and types I and II 15-hydroxyprostaglandin dehydrogenase in swine kidney medulla and cortex

Sc¹,

Ss²,

Katzen³

et al. 1975

Biochemistry

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“…Concentrations of prostaglandin (PGE 2 ) in the culture media were determined by radio-immunoassay (RIA), utilising dextran-coated charcoal to separate bound from free 3 H-PGE 2 , essentially as described elsewhere (Levine et al, 1971). The commercial antiserum used was cross-reactive with PGE 1 .…”

Section: Prostaglandin Analysismentioning

confidence: 99%

Inhibition of Tendon Cell Proliferation and Matrix Glycosaminoglycan Synthesis by Non-Steroidal Anti-Inflammatory Drugs in vitro

Riley

Cox

Harrall

et al. 2001

Journal of Hand Surgery

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The purpose of this study was to investigate the effects of some commonly used non-steroidal anti-inflammatory drugs (NSAIDs) on human tendon. Explants of human digital flexor and patella tendons were cultured in medium containing pharmacological concentrations of NSAIDs. Cell proliferation was measured by incorporation of 3H-thymidine and glycosaminoglycan synthesis was measured by incorporation of 35S-Sulphate. Diclofenac and aceclofenac had no significant effect either on tendon cell proliferation or glycosaminoglycan synthesis. Indomethacin and naproxen inhibited cell proliferation in patella tendons and inhibited glycosaminoglycan synthesis in both digital flexor and patella tendons. If applicable to the in vivo situation, these NSAIDs should be used with caution in the treatment of pain after tendon injury and surgery

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“…Prostaglandins of the E group were determined by a sensitive radioimmunotechnique described by Levine et al (9) and Gutierrez-Cernosek et al (7) and using a commercially available reagent kit (Clinical Assays, lnc., Cambridge, Mass., USA). Because of the low protein content of the blister fluid and its predilution ( I : 10), deproteinization of the specimen was not necessary.…”

Section: Determi11atio11 Of Pge Activitymentioning

confidence: 99%