Specificity and cross-reactivity of staphylococcal enterotoxin A monoclonal antibodies with enterotoxins B, C1, D, and E

Abstract

The cross-reactivity of monoclonal antibodies produced against staphylococcal enterotoxin A with purified and crude enterotoxins B, C,, D, and E and the specificity of such reactions were evaluated by the indirect enzyme-linked immunosorbent assay and immunoblotting of Western blots (from sodium dodecyl sulfatepolyacrylamide gel electrophoresis) followed by autoradiography. Purified and crude enterotoxins B were also tested with polyclonal antibodies. Specificity of reactivity was demonstrated by immunoblotting of crude enterotoxin A, crude enterotoxin A treated with trypsin, crude enterotoxin E, and also with crude A, B, Cl, and D that were pretreated with Sepharose-4B-linked normal rabbit immunoglobulin G to remove protein A. A band corresponding to each staphylococcal enterotoxin was seen with monoclonal antibodies under all conditions tested and also with crude and purified enterotoxin B with two different (rabbit and goat) polyclonal antisera.

Murine monoclonal antibodies against staphylococcal enterotoxin B: production and characterization

“…1). This fact was previously described by Edwin et al [7] for a mAb reacting with all the five serovars of SE. mAb B13 reacts more strongly with the toxin than with the precursor, while B5 reacts similary with both bands (Fig.…”

“…The mAbs did not react with BSA when used as coating antigen (3% in PB). However, the reactivity noted in ELISA could not always be interpreted as being specific to SE [7,8]. Thus mAbs were also analyzed by immunoblotting to demon- 2b 320 20 480 106 B3 1 640 40 960 ND B4 1 160 20 480 107 B5 2b 160 10240 104 B6 1 160 1 280 ND B7 2b 160 640 107 B8 1 1 280 10 240 103 B9 1 64(1 2 560 ND BI0 1 160 2560 ND BII 1 320 40960 104 B12 2b 40 640 ND BI3 1 120 1280 ND B14 2b 320 1280 strate the specific binding of the mAbs to the SEs.…”

“…There are many reports on the development and use of monoclonal antibodies (mAbs) produced against SEs [2][3][4][5][6][7][8][9][10][11][12][13][14][15][16][17][18]. mAbs to all of the identified enterotoxins have been prepared [2], either specific or cross-reactive.…”

“…Meyer et al [14] prepared a monoclonal antibody which reacts with all of the identified enterotoxins, but it also reacts non-specifically with other proteins [1,19]. Edwin et al [7] produced mAbs which reacted specifically with SEA, SEB, SECt, SED and SEE.…”

Production and characterization of anti-peptide monoclonal antibodies with specificity for staphylococcal enterotoxins A and B

“…Several ELISA methods for detection and quantitation of SE using Mabs have been described and many of them have a high sensitivity (Thompson et al, 1984(Thompson et al, , 1986Lapeyre et al, 1987;Shinagawa et al, 1991). However, because of the high sequence similarities between SEA, SED and SEE and between SEB and SECl-3, some Mabs have exhibited cross-reactivity with several SE (Lapeyre et al, 1987;Edwin et al, 1986;Goyache et al, 1992). This is of no consequence when the detection of SE is desired rather than a specific identification.…”